Human Gene Set: BRACHAT_RESPONSE_TO_CAMPTOTHECIN_DN

For the Mouse gene set with the same name, see BRACHAT_RESPONSE_TO_CAMPTOTHECIN_DN

Standard name BRACHAT_RESPONSE_TO_CAMPTOTHECIN_DN
Systematic name M1625
Brief description Genes specifically down-regulated in FL5.12 cells (pro-B lymphocyte) by camptothecin [PubChem=2538].
Full description or abstract DNA microarrays are powerful tools for the analysis of gene expression on a genomic scale. The importance of individual regulatory events for the process under study can however not be deduced unequivocally without additional experiments. We devised a strategy to identify central regulators of cancer drug responses by combining the results of microarray experiments with efficient methods for phenotypic testing of candidate genes. We exposed murine FL5.12 pro-B cells to cisplatin, camptothecin, methotrexate or paclitaxel, respectively and analysed the patterns of gene expression with cDNA microarrays. Drug-specific regulatory events as well as intersections between different apoptotic pathways, including previously studied responses to staurosporine and interleukin-3 (IL-3) deprivation, were identified. Genes shared by at least three pathways were chosen for further analysis. Ectopic expression of three such genes, TEAP, GP49B, and Lipin1 was found to have an anti-proliferative effect on pro-B cells. Interestingly, we identified hemoglobin alpha as a strong pro-apoptotic regulator. While hemoglobin-expressing cells were growing normally in the presence of IL-3, they displayed accelerated apoptosis with similar kinetics as Bax overexpressing cells upon IL-3 removal. The pro-apoptotic effect of hemoglobin was suppressed by Bcl-2 and was characterized by enhanced stimulation of caspase activity.
Collection C2: Curated
      CGP: Chemical and Genetic Perturbations
Source publication Pubmed 12447701   Authors: Brachat A,Pierrat B,Xynos A,Brecht K,Simonen M,Brüngger A,Heim J
Exact source Table 1 & 2: Camptothecin
Related gene sets (show 4 additional gene sets from the source publication)
External links
Filtered by similarity ?
Source species Mus musculus
Contributed by John Newman (University of Washington)
Source platform or
identifier namespace
MOUSE_SEQ_ACCESSION
Dataset references  
Download gene set format: grp | gmt | xml | json | TSV metadata
Compute overlaps ? (show collections to investigate for overlap with this gene set)
Compendia expression profiles ? NG-CHM interactive heatmaps
(Please note that clustering takes a few seconds)
GTEx compendium
Human tissue compendium (Novartis)
Global Cancer Map (Broad Institute)
NCI-60 cell lines (National Cancer Institute)

Legacy heatmaps (PNG)
GTEx compendium
Human tissue compendium (Novartis)
Global Cancer Map (Broad Institute)
NCI-60 cell lines (National Cancer Institute)
Advanced query Further investigate these 42 genes
Gene families ? Categorize these 42 genes by gene family
Show members (show 50 source identifiers mapped to 42 genes)
Version history 3.1: First introduced

See MSigDB license terms here. Please note that certain gene sets have special access terms.