Gene Set: CHIARADONNA_NEOPLASTIC_TRANSFORMATION_CDC25_UP

Standard name CHIARADONNA_NEOPLASTIC_TRANSFORMATION_CDC25_UP
Systematic name M12828
Brief description Genes up-regulated in reverted NIH3T3 cells (fibroblasts transformed by activated KRAS [GeneID=3845] which then reverted to normal cells upon stable over-expression of a dominant negative form of CDC25 [GeneID=5923]) vs normal fibroblasts.
Full description or abstract Mutational activation of ras genes is required for the onset and maintenance of different malignancies. Here we show, using a combination of molecular physiology, nutritional perturbations and transcriptional profiling, that full penetrance of phenotypes related to oncogenic Ras activation, including the shift of carbon metabolism towards fermentation and upregulation of key cell cycle regulators, is dependent upon glucose availability. These responses are induced by Ras activation, being specifically reverted by downregulation of the Ras pathway obtained through the expression of a dominant-negative Ras-specific guanine nucleotide exchange protein. Our data allow to link directly to ras activation the alteration in energy metabolism of cancer cells, their fragility towards glucose shortage and ensuing apoptotic death.
Collection C2: curated gene sets
      CGP: chemical and genetic perturbations
Source publication Pubmed 16607279   Authors: Chiaradonna F,Sacco E,Manzoni R,Giorgio M,Vanoni M,Alberghina L
Exact source Table 1S: DN-wt Up regulated genes
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Organism Mus musculus
Contributed by Lauren Kazmierski (MSigDB Team)
Source platform AFFY_MG_U74
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