Human Gene Set: HADDAD_T_LYMPHOCYTE_AND_NK_PROGENITOR_DN


Standard name HADDAD_T_LYMPHOCYTE_AND_NK_PROGENITOR_DN
Systematic name M999
Brief description Genes down-regulated in hematopoietic progenitor cells (HPC) of T lymphocyte and NK (natural killer) lineage.
Full description or abstract The early stages of human lymphopoiesis are poorly characterized. Here, we compared the lymphoid potential of a novel umbilical cord blood CD34(+)CD45RA(hi)CD7(+) hematopoietic progenitor cell (HPC) population with that of CD34(+)CD45RA(hi)Lin(-)CD10(+) HPCs, previously proposed as candidate common lymphoid progenitors. Limiting-dilution and clonal analysis, fetal thymic organ cultures, and culture onto Notch ligand Delta-like-1-expressing OP9 cells, showed that although CD34(+)CD45RA(hi)CD7(+) HPCs could generate cells of the 3 lymphoid lineages, their potential was skewed toward the T/natural killer (T/NK) lineages. In contrast, CD34(+)CD45RA(hi)Lin(-)CD10(+) HPCs predominantly exhibited a B-cell potential. Gene expression profiling with DNA microarrays confirmed that CD34(+)CD45RA(hi)CD7(+) HPCs selectively expressed T-lymphoid and NK lineage-committed genes while retaining expression of genes affiliated to the granulomonocytic lineage, whereas CD34(+)CD45RA(hi)Lin(-)CD10(+) HPCs displayed a typical pro-B-cell transcription profile and essentially lacked genes unrelated to the B lineage. In addition, both populations could be generated in vitro from CD34(+)CD45RA(int)CD7(-) and CD34(+)CD45RA(hi)Lin(-) HPCs with mixed lymphomyeloid potential, from which they emerged independently with different growth/differentiation factor requirements. These findings indicate that CD34(+)CD45RA(hi)CD7(+) and CD34(+)CD45RA(hi)Lin(-)CD10(+) HPCs correspond to multipotent early lymphoid progenitors polarized toward either the T/NK or B lineage, respectively.
Collection C2: Curated
      CGP: Chemical and Genetic Perturbations
Source publication Pubmed 15331438   Authors: Haddad R,Guardiola P,Izac B,Thibault C,Radich J,Delezoide AL,Baillou C,Lemoine FM,Gluckman JC,Pflumio F,Canque B
Exact source Table 2S
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Source species Homo sapiens
Contributed by Kevin Vogelsang (MSigDB Team)
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identifier namespace		 AFFY_HG_U133
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Version history 3.0: Renamed from HADDAD_CD45CD7_PLUS_VS_MINUS_DN


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