Human Gene Set: LENAOUR_DENDRITIC_CELL_MATURATION_UP


Standard name LENAOUR_DENDRITIC_CELL_MATURATION_UP
Systematic name M4562
Brief description Genes up-regulated during in vitro maturation of CD14+ [GeneID=929] monocytes (day 0) into immature (day 7) and mature dendritic cells (day 14).
Full description or abstract Dendritic cells (DCs) are antigen-presenting cells that play a major role in initiating primary immune responses. We have utilized two independent approaches, DNA microarrays and proteomics, to analyze the expression profile of human CD14(+) blood monocytes and their derived DCs. Analysis of gene expression changes at the RNA level using oligonucleotide microarrays complementary to 6300 human genes showed that approximately 40% of the genes were expressed in DCs. A total of 255 genes (4%) were found to be regulated during DC differentiation or maturation. Most of these genes were not previously associated with DCs and included genes encoding secreted proteins as well as genes involved in cell adhesion, signaling, and lipid metabolism. Protein analysis of the same cell populations was done using two-dimensional gel electrophoresis. A total of 900 distinct protein spots were included, and 4% of them exhibited quantitative changes during DC differentiation and maturation. Differentially expressed proteins were identified by mass spectrometry and found to represent proteins with Ca(2+) binding, fatty acid binding, or chaperone activities as well as proteins involved in cell motility. In addition, proteomic analysis provided an assessment of post-translational modifications. The chaperone protein, calreticulin, was found to undergo cleavage, yielding a novel form. The combined oligonucleotide microarray and proteomic approaches have uncovered novel genes associated with DC differentiation and maturation and has allowed analysis of post-translational modifications of specific proteins as part of these processes.
Collection C2: Curated
      CGP: Chemical and Genetic Perturbations
Source publication Pubmed 11279020   Authors: Le Naour F,Hohenkirk L,Grolleau A,Misek DE,Lescure P,Geiger JD,Hanash S,Beretta L
Exact source Table 1
Related gene sets (show 1 additional gene sets from the source publication)

(show 13 gene sets from any of these authors)
External links
Filtered by similarity ?
Source species Homo sapiens
Contributed by Kevin Vogelsang (MSigDB Team)
Source platform or
identifier namespace		 AFFY_HuGene
Dataset references  
Download gene set format: grp | gmt | xml | json | TSV metadata
Compute overlaps ? (show collections to investigate for overlap with this gene set)
Compendia expression profiles ? NG-CHM interactive heatmaps
(Please note that clustering takes a few seconds)
GTEx compendium
Human tissue compendium (Novartis)
Global Cancer Map (Broad Institute)
NCI-60 cell lines (National Cancer Institute)

Legacy heatmaps (PNG)
GTEx compendium
Human tissue compendium (Novartis)
Global Cancer Map (Broad Institute)
NCI-60 cell lines (National Cancer Institute)
Advanced query Further investigate these 116 genes
Gene families ? Categorize these 116 genes by gene family
Show members (show 119 source identifiers mapped to 116 genes)
Version history 3.0: Renamed from HOHENKIRK_MONOCYTE_DEND_UP

See MSigDB license terms here. Please note that certain gene sets have special access terms.