Human Gene Set: MACLACHLAN_BRCA1_TARGETS_UP


Standard name MACLACHLAN_BRCA1_TARGETS_UP
Systematic name M17772
Brief description Genes up-regulated in SW480 cells (colon cancer with mutated p53 [GeneID=7157]) upon expression of BRCA1 [GeneID=672] off an adenovirus vector.
Full description or abstract The breast and ovarian cancer susceptibility gene product BRCA1 has been reported to be expressed in a cell cycle-dependent manner; possess transcriptional activity; associate with several proteins, including the p53 tumor suppressor; and play an integral role in certain types of DNA repair. We show here that ectopic expression of BRCA1 using an adenovirus vector (Ad-BRCA1) leads to dephosphorylation of the retinoblastoma protein accompanied by a decrease in cyclin-dependent kinase activity. Flow cytometric analysis on Ad-BRCA1-infected cells revealed a G(1) or G(2) phase accumulation. High density cDNA array screening of colon, lung, and breast cancer cells identified several genes affected by BRCA1 expression in a p53-independent manner, including DNA damage response genes and genes involved in cell cycle control. Notable changes included induction of the GADD45 and GADD153 genes and a reduction in cyclin B1 expression. Therefore, BRCA1 has the potential to modulate the expression of genes and function of proteins involved in cell cycle control and DNA damage response pathways.
Collection C2: Curated
      CGP: Chemical and Genetic Perturbations
Source publication Pubmed 10644742   Authors: MacLachlan TK,Somasundaram K,Sgagias M,Shifman Y,Muschel RJ,Cowan KH,El-Deiry WS
Exact source Table 1: Induced genes
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Source species Homo sapiens
Contributed by John Newman (University of Washington)
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identifier namespace		 HUMAN_GENE_SYMBOL
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Version history 3.0: Renamed from BRCA1_SW480_UP

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