Standard name |
MULLIGAN_NTF3_SIGNALING_VIA_INSR_AND_IGF1R_UP |
Systematic name |
M1593 |
Brief description |
Genes similarly up-regulated in 3T3-L1 cells (fibroblasts able to differentiate to adipocytes) upon stimulation of INSR or IGFR1 by NTF3 [GeneID=3643;3480;4908]. |
Full description or abstract |
Insulin and insulin-like growth factor-1 (IGF-1) act through highly homologous receptors that engage similar intracellular signaling pathways, yet these hormones serve largely distinct physiological roles in the control of metabolism and growth, respectively. In an attempt to uncover the molecular mechanisms underlying their divergent functions, we compared insulin receptor (IR) and IGF-1 receptor (IGF-1R) regulation of gene expression by microarray analysis, using 3T3-L1 cells expressing either TrkC/IR or TrkC/IGF-1R chimeric receptors to ensure the highly selective activation of each receptor tyrosine kinase. Following stimulation of the chimeric receptors for 4 h, we detected 11 genes to be differentially regulated, of which 10 were up-regulated to a greater extent by the IGF-1R. These included genes involved in adhesion, transcription, transport, and proliferation. The expression of mRNA encoding heparin-binding epidermal growth factor-like growth factor (HB-EGF), a potent mitogen, was markedly increased by IGF-1R but not IR activation. This effect was dependent on MAPK, but not phosphatidylinositol 3-kinase, and did not require an autocrine loop through the epidermal growth factor receptor. HB-EGF mitogenic activity was detectable in the medium of 3T3-L1 preadipocytes expressing activated IGF-1R but not IR, indicating that the transcriptional response is accompanied by a parallel increase in mature HB-EGF protein. The differential abilities of the IR and IGF-1R tyrosine kinases to stimulate the synthesis and release of a growth factor may provide, at least in part, an explanation for the greater role of the IGF-1R in the control of cellular proliferation. |
Collection |
C2: curated gene sets CGP: chemical and genetic perturbations |
Source publication |
Pubmed 12213819 Authors: Mulligan C,Rochford J,Denyer G,Stephens R,Yeo G,Freeman T,Siddle K,O'Rahilly S |
Exact source |
Table 1A |
Related gene sets |
(show 1 additional gene sets from the source publication)
(show 6 gene sets from the same authors)
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External links |
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Filtered by similarity  |
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Organism |
Mus musculus |
Contributed by |
John Newman (University of Washington) |
Source platform |
MOUSE_SEQ_ACCESSION |
Dataset references |
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format: grp | gmt | xml | json | TSV metadata |
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Compendia expression profiles  |
GTEx compendium
Human tissue compendium (Novartis)
Global Cancer Map (Broad Institute)
NCI-60 cell lines (National Cancer Institute)
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these 22 genes
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Gene families  |
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22 genes by gene family
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Show members |
(show 23 members mapped to 22 genes)
Original Member |
NCBI (Entrez) Gene Id |
Gene Symbol |
Gene Description |
AA615831 |
3308 |
HSPA4 |
heat shock protein family A (Hsp70) memb... |
AB011030 |
64388 |
GREM2 |
"gremlin 2, DAN family BMP antagonist [S... |
AI117157 |
6480 |
ST6GAL1 |
"ST6 beta-galactoside alpha-2,6-sialyltr... |
AI785289 |
26354 |
GNL3 |
G protein nucleolar 3 [Source:HGNC Symbo... |
AI836083 |
9188 |
DDX21 |
DExD-box helicase 21 [Source:HGNC Symbol... |
AI838915 |
55052 |
MRPL20 |
mitochondrial ribosomal protein L20 [Sou... |
AI843466 |
27230 |
SERP1 |
stress associated endoplasmic reticulum ... |
AI845934 |
10969 |
EBNA1BP2 |
EBNA1 binding protein 2 [Source:HGNC Sym... |
AI846023 |
10123 |
ARL4C |
ADP ribosylation factor like GTPase 4C [... |
AI852608 |
10171 |
RCL1 |
RNA terminal phosphate cyclase like 1 [S... |
AI852985 |
27346 |
TMEM97 |
transmembrane protein 97 [Source:HGNC Sy... |
AI853476 |
79084 |
WDR77 |
WD repeat domain 77 [Source:HGNC Symbol;... |
AW120925 |
29925 |
GMPPB |
GDP-mannose pyrophosphorylase B [Source:... |
AW121447 |
10528 |
NOP56 |
NOP56 ribonucleoprotein [Source:HGNC Sym... |
AW123694 |
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AW123801 |
23603 |
CORO1C |
coronin 1C [Source:HGNC Symbol;Acc:HGNC:... |
D28941 |
6484 |
ST3GAL4 |
"ST3 beta-galactoside alpha-2,3-sialyltr... |
D84096 |
9100 |
USP10 |
ubiquitin specific peptidase 10 [Source:... |
M19681 |
6347 |
CCL2 |
C-C motif chemokine ligand 2 [Source:HGN... |
M35970 |
4830 |
NME1 |
NME/NM23 nucleoside diphosphate kinase 1... |
U88064 |
646 |
BNC1 |
basonuclin 1 [Source:HGNC Symbol;Acc:HGN... |
X98511 |
6427 |
SRSF2 |
serine and arginine rich splicing factor... |
Z31362 |
140809 |
SRXN1 |
sulfiredoxin 1 [Source:HGNC Symbol;Acc:H... |
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Version history |
3.1: First introduced
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