Systematic name M2601
Brief description Genes up-regulated in the human skin cells at 4 h after exprosure to 5 Gy dose of ionizing radiation.
Full description or abstract Although skin is usually exposed during human exposures to ionizing radiation, there have been no thorough examinations of the transcriptional response of skin fibroblasts and keratinocytes to radiation. The transcriptional response of quiescent primary fibroblasts and keratinocytes exposed to from 10 cGy to 5 Gy and collected 4 h after treatment was examined. RNA was isolated and examined by microarray analysis for changes in the levels of gene expression. Exposure to ionizing radiation altered the expression of 279 genes across both cell types. Changes in RNA expression could be arranged into three main categories: (1) changes in keratinocytes but not in fibroblasts, (2) changes in fibroblasts but not in keratinocytes, and (3) changes in both. All of these changes were primarily of p53 target genes. Similar radiation-induced changes were induced in immortalized fibroblasts or keratinocytes. In separate experiments, protein was collected and analyzed by Western blotting for expression of proteins observed in microarray experiments to be overexpressed at the mRNA level. Both Q-PCR and Western blot analysis experiments validated these transcription changes. Our results are consistent with changes in the expression of p53 target genes as indicating the magnitude of cell responses to ionizing radiation.
Collection C2: Curated
      CGP: Chemical and Genetic Perturbations
Source publication Pubmed 19580510   Authors: Warters RL,Packard AT,Kramer GF,Gaffney DK,Moos PJ
Exact source Suppl. Data File: sheet 'SAM identified genes'
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Source species Homo sapiens
Contributed by Itai Pashtan (Dana-Farber Cancer Institute)
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Version history 3.1: First introduced

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