STANDARD_NAME BOUDOUKHA_BOUND_BY_IGF2BP2 SYSTEMATIC_NAME M2525 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/BOUDOUKHA_BOUND_BY_IGF2BP2 NAMESPACE MOUSE_SEQ_ACCESSION DESCRIPTION_BRIEF Transcripts bound to IGF2BP2 [GeneID=10644] complexes and differentially regulated in myoblasts with IGF2BP2 [GeneID=10644] knockdown by RNAi. DESCRIPTION_FULL Insulin-like growth factor 2 (IGF-2) mRNA-binding proteins (IMPs) are a family of posttranscriptional regulatory factors with well-understood roles in embryonic development and cancer but with poorly characterized functions in normal adult cells and tissues. We now show that IMP-2, the most ubiquitously expressed member of the family, is abundant in human and mouse adult skeletal myoblasts, where it is indispensable for cell motility and for stabilization of microtubules. To explore the functions of IMP-2, we analyzed the transcripts that were differentially regulated in IMP-2-depleted myoblasts and bound to IMP-2 in normal myoblasts. Among them were the mRNAs of PINCH-2, an important mediator of cell adhesion and motility, and MURF-3, a microtubule-stabilizing protein. By gain- and loss-of-function assays and gel shift experiments, we show that IMP-2 regulates the expression of PINCH-2 and MURF-3 proteins via direct binding to their mRNAs. Upregulation of PINCH-2 in IMP-2-depleted myoblasts is the key event responsible for their decreased motility. Our data reveal how the posttranscriptional regulation of gene expression by IMP-2 contributes to the control of adhesion structures and stable microtubules and demonstrate an important function for IMP-2 in cellular motility. PMID 20956565 GEOID AUTHORS Boudoukha S,Cuvellier S,Polesskaya A CONTRIBUTOR Arthur Liberzon CONTRIBUTOR_ORG MSigDB Team EXACT_SOURCE Table 1S FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS AK009928,AK010113,AK013984,AK017383,AK021143,BC022589,BC026977,BC027311,BG063913,BQ031149,BQ175265,NM_002835,NM_007475,NM_007504,NM_007631,NM_007632,NM_007749,NM_007750,NM_007778,NM_007945,NM_007990,NM_008445,NM_008549,NM_008556,NM_008681,NM_009079,NM_009091,NM_009094,NM_009143,NM_009255,NM_009517,NM_009701,NM_009729,NM_009831,NM_009964,NM_009986,NM_010133,NM_010178,NM_010216,NM_010239,NM_010329,NM_010450,NM_010478,NM_010760,NM_010839,NM_011313,NM_011619,NM_011639,NM_011692,NM_011865,NM_011877,NM_013525,NM_013608,NM_013647,NM_013750,NM_013813,NM_013898,NM_016721,NM_016753,NM_016794,NM_016800,NM_018730,NM_018829,NM_019443,NM_019484,NM_019571,NM_019682,NM_019780,NM_019912,NM_019922,NM_019976,NM_020582,NM_021447,NM_021467,NM_021515,NM_023123,NM_023166,NM_023422,NM_023732,NM_024171,NM_024277,NM_025278,NM_025287,NM_025318,NM_025362,NM_025520,NM_025589,NM_025897,NM_025933,NM_025946,NM_025963,NM_025966,NM_025983,NM_026123,NM_026160,NM_026249,NM_027215,NM_053090,NM_080848,NM_133655,NM_133672,NM_133685,NM_133697,NM_133748,NM_133806,NM_133815,NM_133928,NM_133948,NM_134007,NM_134014,NM_134076,NM_134095,NM_138721,NM_139061,NM_144862,NM_145486,NM_172151 GENE_SYMBOLS KLF9,,ABRACL,TNKS2,,ISCA2,NAPG,,,,GID8,PTPN12,RPLP0,ATP2A1,CCND1,CCND3,COX7C,COX8A,CSF1,EPS8,FAU,KIF3C,MAN2A1,PEA15,NDRG1,RPL22,RPS15,RPS4X,SDF2,SERPINE2,ZMAT3,AQP5,ATP6V0C,CCNG1,CRYAB,CUX1,EN1,SRSF10,VEGFD,FTH1,PDPN,HOXA11,HSPA1B,,CMC4,S100A6,TNNT2,TRIP6,VBP1,PCBP1,PTPN21,GAS5,NACA,RPS16,PHLDA3,EPB41L3,TIMM8A,IQGAP1,LXN,VAMP8,VTI1B,RPL36,AP3M1,NDUFA1,ALYREF,TSPAN5,DYNLL1,VPS29,UBE2D2,CRTAP,PSRC1,ATP5MF,TRIM54,TNNI1,AK1,,FAM89B,H2BC10,ABCB6,SEC61B,RPS27A,GNG12,SPOP,EMC6,DNAJC30,LSM5,,RRP8,HIGD2A,ROMO1,RPS10,C6orf226,ATP5F1E,UNC50,MAP1LC3B2,,TMEM147,HYCC1,WDR5,CD81,VPS26A,RAB31,SMIM14,INSIG2,UAP1,LBR,CHCHD4,PSIP1,CISD1,XPO1,ABHD4,DESI1,LSM10,VPS54,LIMS2,MARCHF2,ZDHHC8 FOUNDER_NAMES