STANDARD_NAME BROWNE_HCMV_INFECTION_10HR_DN SYSTEMATIC_NAME M8416 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/BROWNE_HCMV_INFECTION_10HR_DN NAMESPACE AFFY_HG_U95 DESCRIPTION_BRIEF Genes down-regulated in primary fibroblast cell culture after infection with HCMV (AD169 strain) at 10 h time point that were not down-regulated at the previous time point, 8 h. DESCRIPTION_FULL The effect of human cytomegalovirus (HCMV) infection on cellular mRNA accumulation was analyzed by gene chip technology. During a 48-h time course after infection of human diploid fibroblasts, 1,425 cellular mRNAs were found to be up-regulated or down-regulated by threefold or greater in at least two consecutive time points. Several classes of genes were prominently affected, including interferon response genes, cell cycle regulators, apoptosis regulators, inflammatory pathway genes, and immune regulators. The number of mRNAs that were up-regulated or down-regulated were roughly equal over the complete time course. However, for the first 8 h after infection, the number of up-regulated mRNAs was significantly less than the number of down-regulated mRNAs. By analyzing the mRNA expression profile of cells infected in the presence of cycloheximide, it was found that a minimum of 25 mRNAs were modulated by HCMV in the absence of protein synthesis. These included mRNAs encoded by a small number of interferon-responsive genes, as well as beta interferon itself. Cellular mRNA levels in cytomegalovirus-infected cells were compared to the levels in cells infected with UV-inactivated virus. The inactivated virus caused the up-regulation of a much greater number of mRNAs, many of which encoded proteins with antiviral roles, such as interferon-responsive genes and proinflammatory cytokines. These data argue that one or more newly synthesized viral gene products block the induction of antiviral pathways that are triggered by HCMV binding and entry. PMID 11711622 GEOID GSE675 AUTHORS Browne EP,Wing B,Coleman D,Shenk T CONTRIBUTOR John Newman CONTRIBUTOR_ORG University of Washington EXACT_SOURCE Table 1S: 10hpi <= -3 & Diff Call [10 hpi] = D, MD FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS 1088_at,1288_s_at,1530_g_at,201_s_at,31669_s_at,31672_g_at,31886_at,31910_at,32025_at,32118_at,32262_at,32627_at,32639_at,33010_at,33220_at,33378_at,34015_at,34706_at,34908_at,35510_at,35655_at,35773_i_at,36324_at,36525_at,36550_at,36690_at,36849_at,37034_at,37156_at,37303_at,37538_at,37558_at,37746_r_at,37762_at,38013_at,38333_at,38667_at,39096_at,39218_at,39391_at,39421_at,39771_at,40030_at,40083_at,40202_at,40516_at,40726_at,40730_at,41049_at,41086_at,41172_at,41250_at,41363_at,41574_at,41665_at,41706_at,514_at,851_s_at,854_at,872_i_at,943_at,AFFX-TrpnX-5_at GENE_SYMBOLS BDNF,EEF1A1,FRY,B2M,HOXA11,RBMS1,NT5E,,TCF7L2,RAD17,METTL13,RANBP6,NUP42,SLC5A4,ZSCAN26,NIPBL,PTGFR,MGA,TTC30A,SCN8A,ANKRD28,NDUFB7,ADORA2B,FBXL2,RIN2,NR3C1,ARHGAP29,ANP32A,ETV1,PARP4,ENSG00000291006,IGF2BP3,DENND2B,EMP1,MTUS1,OGG1,GLOD4,SON,ZNF23,STAMBP,RUNX1,RHOBTB1,PRKY,SETX,KLF9,AHR,KIF11,PNOC,IRS1,RGS20,RDH11,AIMP2,GEMIN2,PNN,PCF11,AMACR,CBLB,IRS1,BLK,IRS1,RUNX1, FOUNDER_NAMES