STANDARD_NAME LEE_INTRATHYMIC_T_PROGENITOR SYSTEMATIC_NAME M8213 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/LEE_INTRATHYMIC_T_PROGENITOR NAMESPACE AFFY_HG_U133 DESCRIPTION_BRIEF Genes enriched in the intrathymic T progenitor (ITTP) cells compared to all other T lymphocyte differentiation stages. DESCRIPTION_FULL To develop a comprehensive catalogue of phenotypic and functional parameters of human CD4(+) T cell differentiation stages, we have performed microarray gene expression profiling on subpopulations of human thymocytes and circulating naive CD4(+) T cells, including CD3(-)CD4(+)CD8(-) intrathymic T progenitor cells, CD3(int)CD4(+)CD8(+) 'double positive' thymocytes, CD3(high)CD4(+)CD8(-) 'single positive' thymocytes, CD3(+)CD4(+)CD8(-) CD45RA(+)CD62L(+) naive T cells from cord blood and CD3(+)CD4(+)CD8(-) CD45RA(+)CD62L(+) naive T cells from adult blood. These subpopulations were sort-purified to >98% purity and their expressed RNAs were analyzed on Affymetrix Human Genome U133 arrays. Comparison of gene expression signals between these subpopulations and with early passage fetal thymic stromal cultures identify: (i) transcripts that are preferentially expressed in human CD4(+) T cell subpopulations and not in thymic stromal cells; (ii) major shifts in gene expression as progenitor T cells mature into progeny; (iii) preferential expression of transcripts at the progenitor cell stage with plausible relevance to the regulation of expansion and differentiation of these cells; and (iv) preferential expression of potential markers of recent thymic emigrants in naive-phenotype CD4(+) T cells from cord blood. Further evaluation of these findings may lead to a better definition of human thymopoiesis as well as to improved approaches to monitor and to augment the function of this important organ of T cell production. PMID 15210650 GEOID AUTHORS Lee MS,Hanspers K,Barker CS,Korn AP,McCune JM CONTRIBUTOR Arthur Liberzon CONTRIBUTOR_ORG MSigDB Team EXACT_SOURCE Table 3-3S: SOM C2: ITTP vs other FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS 201328_at,202016_at,202252_at,203817_at,204730_at,205674_x_at,207426_s_at,207434_s_at,209550_at,209905_at,212070_at,212589_at,212592_at,214651_s_at,219036_at,220146_at,221942_s_at,222288_at,222303_at,224150_s_at,224847_at,228816_at,229530_at,235371_at,235597_s_at,235733_at,241074_at,244764_at GENE_SYMBOLS ETS2,MEST,RAB13,GUCY1B1,RIMS3,FXYD2,TNFSF4,FXYD2,NDN,HOXA9,ADGRG1,RRAS2,JCHAIN,HOXA9,CEP70,TLR7,GUCY1A1,,,CEP70,CDK6,ATP6AP1L,GUCY1A1,GXYLT2,RGPD1,GXYLT2,,HIVEP3 FOUNDER_NAMES