STANDARD_NAME LIANG_HEMATOPOIESIS_STEM_CELL_NUMBER_LARGE_VS_TINY_DN SYSTEMATIC_NAME M870 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/LIANG_HEMATOPOIESIS_STEM_CELL_NUMBER_LARGE_VS_TINY_DN NAMESPACE AFFY_MG_U74 DESCRIPTION_BRIEF Genes down-regulated in LSK cells (bone marrow) as a function of a QTL for the size of hematopoietic stem cell (HSC) population: comparison of congenic B.D. chr3 (BD, large HSC size) vs parental B6 strain (tiny HSC size). DESCRIPTION_FULL We mapped quantitative trait loci that accounted for the variation in hematopoietic stem cell (HSC) numbers between young adult C57BL/6 (B6) and DBA/2 (D2) mice. In reciprocal chromosome 3 congenic mice, introgressed D2 alleles increased HSC numbers owing to enhanced proliferation and self-renewal and reduced apoptosis, whereas B6 alleles had the opposite effects. Using oligonucleotide arrays, real-time PCR and protein blots, we identified latexin (Lxn), a gene whose differential transcription and expression was associated with the allelic differences. Expression was inversely correlated with the number of HSCs; therefore, ectopic expression of Lxn using a retroviral vector decreased stem cell population size. We identified clusters of SNPs upstream of the Lxn transcriptional start site, at least two of which are associated with potential binding sites for transcription factors regulating stem cells. Thus, promoter polymorphisms between the B6 and D2 alleles may affect Lxn gene expression and consequently influence the population size of hematopoietic stem cells. PMID 17220891 GEOID AUTHORS Liang Y,Jansen M,Aronow B,Geiger H,Van Zant G CONTRIBUTOR Leona Saunders CONTRIBUTOR_ORG MSigDB Team EXACT_SOURCE Table 3S FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS 100391_at,100429_at,100443_at,100708_at,100901_at,101077_at,101507_at,101529_g_at,102011_at,102260_at,103066_at,103257_at,103688_at,104141_at,104337_f_at,104572_at,160526_s_at,160685_at,160738_at,160854_at,160921_at,161055_r_at,161287_f_at,92185_at,93134_at,93498_s_at,94010_g_at,94224_s_at,94297_at,94419_at,94476_at,94753_at,94778_at,95052_at,95077_at,96065_at,96232_at,96806_at,97318_at,97565_r_at,98388_at,98500_at,98600_at,98807_at,98892_at,98928_at,99138_at GENE_SYMBOLS MAPK8,PPOX,BCAT2,H3-3A,HCFC1,SLC4A1,SCNM1,TCEA1,CEP89,GFI1B,CMPK2,TLCD4,SOWAHA,DESI1,PKP2,TUT7,CREM,,POLRMT,MAP3K7,ACSS1,SPRYD7,,ARL4C,NPTX1,APLP2,ELOC,IFI16,FKBP5,SLC19A1,TAF1D,GNA15,ALDH1A1,C1QTNF12,RABGGTB,LXN,CUL2,LPIN2,DTD1,,,IL1RL1,S100A11,GNAT2,LPIN1,PTPRCAP,RCC1 FOUNDER_NAMES