STANDARD_NAME SCHLINGEMANN_SKIN_CARCINOGENESIS_TPA_DN SYSTEMATIC_NAME M1623 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/SCHLINGEMANN_SKIN_CARCINOGENESIS_TPA_DN NAMESPACE MOUSE_SEQ_ACCESSION DESCRIPTION_BRIEF Down-regulated in murine dorsal skin cells at 6 h after treatment with the phorbol ester carcinogen TPA [PubChem=4792]. DESCRIPTION_FULL Malignant transformation of mouse skin by chemical carcinogens and tumour promoters, such as the phorbol ester 12-O-tetradecanoylphorbol-13-acetate (TPA), is a multistage process that leads to squamous cell carcinoma (SCC) formation. In an effort to identify tumour-associated genes, we studied the influence of short-term TPA-treatment on the gene expression profile of murine skin. A comprehensive microarray with some 5,000 murine gene specific cDNA fragments was established and hybridised with pooled RNA derived from control and TPA-treated dorsal skin samples. Of these genes, 54 were up- and 35 were down-regulated upon TPA application. Additionally, we performed suppression subtractive hybridisation (SSH) with respective RNA pools to generate and analyse a cDNA library enriched for TPA-inducible genes. Expression data of selected genes were confirmed by quantitative real-time PCR and Northern blot analysis. Comparison of microarray and SSH data revealed that 26% of up-regulated genes identified by expression profiling matched with those present in the SSH library. Besides numerous known genes, we identified a large set of unknown cDNAs that represent previously unrecognised TPA-regulated genes in murine skin with potential function in tumour promotion. Additionally, some TPA-induced genes, such as Sprr1A, Saa3, JunB, Il4ralpha, Gp38, RalGDS and Slpi exhibit high basal level in advanced stages of skin carcinogenesis, suggesting that at least a subgroup of the identified TPA-regulated genes may contribute to tumour progression and metastasis. PMID 12640676 GEOID AUTHORS Schlingemann J,Hess J,Wrobel G,Breitenbach U,Gebhardt C,Steinlein P,Kramer H,Fürstenberger G,Hahn M,Angel P,Lichter P CONTRIBUTOR John Newman CONTRIBUTOR_ORG University of Washington EXACT_SOURCE Table 4 FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS AB013398,AB030203,AB041555,AF053943,AF171086,AF345994,AF426314,AJ223855,AK002326,AK004732,AK007427,AK008086,AU017498,AV280875,BC014853,D16195,K02237,M19729,M20683,M36084,NM_009381,NM_010455,NM_016874,NM_021791,NM_027940,NM_030886,NM_053178,U08020,U14556,X61600,Z22660 GENE_SYMBOLS CCL27,ITM2B,RSRP1,AEBP1,CCL21,COL1A2,SH3GLB2,TCAP,GMPR,MXRA8,CCDC28B,GPT,,TMEM255A,PLEKHA5,GRN,,CD247,,,THRSP,HOXA7,DEAF1,DOC2GP,,ANKRD17,ACSBG1,COL1A1,ZBTB17,ENO3,APOC1 FOUNDER_NAMES