STANDARD_NAME ALCALA_APOPTOSIS SYSTEMATIC_NAME M16169 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/ALCALA_APOPTOSIS NAMESPACE HUMAN_SEQ_ACCESSION DESCRIPTION_BRIEF Genes able to induce cell death in an expression cDNA library screen. DESCRIPTION_FULL Functional annotation of complex genomes requires the development of novel experimental platforms with increased capacity. Here, we describe a high-throughput system designed to identify cDNAs whose overexpression induces morphologically distinct cell death modalities. The methodology incorporates two robotized steps, and relies on coexpression of library clones with GFP to reveal the morphological features presented by the dying cells. By using this system we screened 135 000 cDNA clones and obtained 90 independent molecules. Interestingly, three death categories were identified, namely; apoptotic, vacuolated and autophagic. Among the pro-apoptotic clones, we found four members of the mitochondrial carrier family: the phosphate and adenine nucleotide (type 3) transporters, and the mitochondrial carrier homologs (MTCHs) 1 and 2. Expression of these molecules induced cytochrome c release and caspase-9-dependent death. One of them, the phosphate carrier, was able to interact with members of the permeability transition pore complex ANT1 and VDAC1, and its binding to ANT1 was stabilized in the presence of apoptotic activators. Depletion of this carrier by siRNA delayed cytochrome c mobilization and apoptosis. These results attribute a previously undescribed apoptotic function to the phosphate carrier and, more generally, suggest that a common property of various mitochondrial transporters was exploited during evolution to regulate apoptosis. PMID 17621274 GEOID AUTHORS Alcalá S,Klee M,Fernández J,Fleischer A,Pimentel-Muiños FX CONTRIBUTOR Jessica Robertson CONTRIBUTOR_ORG MSigDB Team EXACT_SOURCE Table 1 FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS AY247836,BC029439,NM_000175,NM_000182,NM_000251,NM_000516,NM_001001586,NM_001002,NM_001190,NM_001225,NM_001402,NM_001636,NM_001664,NM_001686,NM_001778,NM_001861,NM_002005,NM_002046,NM_002116,NM_002462,NM_002627,NM_002635,NM_002872,NM_002881,NM_003001,NM_003374,NM_003400,NM_003754,NM_003978,NM_004034,NM_004131,NM_004221,NM_004228,NM_004310,NM_004331,NM_004355,NM_004604,NM_004819,NM_004960,NM_004965,NM_005015,NM_005333,NM_005698,NM_005760,NM_005926,NM_006054,NM_006137,NM_006335,NM_006407,NM_006476,NM_006597,NM_006639,NM_006755,NM_006871,NM_006912,NM_007104,NM_012073,NM_012123,NM_014298,NM_014341,NM_014342,NM_014376,NM_014607,NM_014765,NM_014855,NM_015135,NM_015143,NM_015214,NM_015665,NM_016002,NM_017490,NM_017811,NM_020194,NM_020946,NM_022136,NM_022821,NM_024056,NM_025176,NM_025228,NM_031301,NM_032837,NM_033292,NM_138761,NM_139354,NM_152272,NM_152872,NM_198437,NM_198517,XM_930748,XM_941317 GENE_SYMBOLS ,TMEM35B,GPI,HADHA,MSH2,GNAS,ATP1A1,RPLP0,BCAT2,CASP4,EEF1A1,SLC25A6,RHOA,ATP5F1B,CD48,COX4I1,FES,GAPDH,HLA-A,MX1,PFKP,SLC25A3,RAC2,RALB,SDHC,VDAC1,XPO1,EIF3F,PSTPIP1,ANXA7,GZMB,IL32,CYTH2,RHOH,BNIP3L,CD74,STX4,SYMPK,FUS,HMGN1,OXA1L,HCCS,SCAMP3,CEBPZ,MFAP1,RTN3,CD7,TIMM17A,ARL6IP5,ATP5MG,HSPA8,CYSLTR1,TALDO1,RIPK3,RIT1,RPL10A,CCT5,MTO1,QPRT,MTCH1,MTCH2,CYFIP2,UBXN4,TOMM20,AP5Z1,NUP205,METAP1,DDHD2,AAAS,SCCPDH,MARK2,UBE2R2,MFF,DENND1A,SAMSN1,ELOVL1,TMEM106C,NINL,TRAF3IP3,APH1B,FAM104A,CASP1,BAX,MATK,CHMP7,FAS,AURKA,TBC1D10C,, FOUNDER_NAMES