STANDARD_NAME AMUNDSON_GAMMA_RADIATION_RESPONSE SYSTEMATIC_NAME M14767 COLLECTION ARCHIVED:C2_CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/AMUNDSON_GAMMA_RADIATION_RESPONSE NAMESPACE UniGene_ID DESCRIPTION_BRIEF Genes down-regulated across the entire panel of NCI-60 cell lines in response to gamma radiation. DESCRIPTION_FULL The 60 cell lines of the National Cancer Institute Anticancer Drug Screen (NCI-60) constitute the most extensively characterized in vitro cancer cell model. They have been tested for sensitivity to more than 100,000 potential chemotherapy agents and have been profiled extensively at the DNA, RNA, protein, functional, and pharmacologic levels. We have used the NCI-60 cell lines and three additional lines to develop a database of responses of cancer cells to ionizing radiation. We compared clonogenic survival, apoptosis, and gene expression response by microarray. Although several studies have profiled relative basal gene expression in the NCI-60, this is the first comparison of large-scale gene expression changes in response to genotoxic stress. Twenty-two genes were differentially regulated in cells with low survival after 2-Gy gamma-rays; 14 genes identified lines more sensitive to 8 Gy. Unlike reported basal gene expression patterns, changes in expression in response to radiation showed little tissue-of-origin effect, except for differentiating the lymphoblastoid cell lines from other cell types. Basal expression patterns, however, discriminated well between radiosensitive and more resistant lines, possibly being more informative than radiation response signatures. The most striking patterns in the radiation data were a set of genes up-regulated preferentially in the p53 wild-type lines and a set of cell cycle regulatory genes down-regulated across the entire NCI-60 panel. The response of those genes to gamma-rays seems to be unaffected by the myriad of genetic differences across this diverse cell set; it represents the most penetrant gene expression response to ionizing radiation yet observed. PMID 18199535 GEOID GSE7505 AUTHORS Amundson SA,Do KT,Vinikoor LC,Lee RA,Koch-Paiz CA,Ahn J,Reimers M,Chen Y,Scudiero DA,Weinstein JN,Trent JM,Bittner ML,Meltzer PS,Fornace AJ Jr CONTRIBUTOR Jessica Robertson CONTRIBUTOR_ORG MSigDB Team EXACT_SOURCE Table 3S: yellow FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS Hs.104019,Hs.110757,Hs.153479,Hs.153704,Hs.153752,Hs.1594,Hs.169840,Hs.170076,Hs.194698,Hs.1973,Hs.23960,Hs.249441,Hs.250822,Hs.270845,Hs.276925,Hs.301613,Hs.334562,Hs.350966,Hs.35101,Hs.424414,Hs.435166,Hs.436912,Hs.442658,Hs.469649,Hs.477879,Hs.494347,Hs.497684,Hs.497741,Hs.525324,Hs.567385,Hs.58974,Hs.591697,Hs.592049,Hs.631699,Hs.652463,Hs.655708,Hs.656,Hs.69360,Hs.75066,Hs.75573,Hs.83758,Hs.93002 GENE_SYMBOLS ,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, FOUNDER_NAMES