STANDARD_NAME	BASAKI_YBX1_TARGETS_DN
SYSTEMATIC_NAME	M14877
COLLECTION	C2:CGP
MSIGDB_URL	https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/BASAKI_YBX1_TARGETS_DN
NAMESPACE	AFFY_HG_U133
DESCRIPTION_BRIEF	Genes down-regulated in SKOC-3 cells (ovarian cancer) after YB-1 (YBX1) [GeneID=4904] knockdown by RNAi.
DESCRIPTION_FULL	Y-box-binding protein 1 (YB-1), which is a member of the DNA-binding protein family containing a cold-shock domain, has pleiotropic functions in response to various environmental stimuli. As we previously showed that YB-1 is a global marker of multidrug resistance in ovarian cancer and other tumor types. To identify YB-1-regulated genes in ovarian cancers, we investigated the expression profile of YB-1 small-interfering RNA (siRNA)-transfected ovarian cancer cells using a high-density oligonucleotide array. YB-1 knockdown by siRNA upregulated 344 genes, including MDR1, thymidylate synthetase, S100 calcium binding protein and cyclin B, and downregulated 534 genes, including CXCR4, N-myc downstream regulated gene 1, E-cadherin and phospholipase C. Exogenous serum addition stimulated YB-1 translocation from the cytoplasm to the nucleus, and treatment with Akt inhibitors as well as Akt siRNA and integrin-linked kinase (ILK) siRNA specifically blocked YB-1 nuclear localization. Inhibition of Akt activation downregulated CXCR4 and upregulated MDR1 (ABCB1) gene expression. Administration of Akt inhibitor resulted in decrease in nuclear YB-1-positive cancer cells in a xenograft animal model. Akt activation thus regulates the nuclear translocation of YB-1, affecting the expression of drug-resistance genes and other genes associated with the malignant characteristics in ovarian cancer cells. Therefore, the Akt pathway could be a novel target of disrupting the nuclear translocation of YB-1 that has important implications for further development of therapeutic strategy against ovarian cancers.
PMID	17072343
GEOID	
AUTHORS	Basaki Y,Hosoi F,Oda Y,Fotovati A,Maruyama Y,Oie S,Ono M,Izumi H,Kohno K,Sakai K,Shimoyama T,Nishio K,Kuwano M
CONTRIBUTOR	Arthur Liberzon
CONTRIBUTOR_ORG	MSigDB Team
EXACT_SOURCE	Table 1S
FILTERED_BY_SIMILARITY	
EXTERNAL_NAMES_FOR_SIMILAR_TERMS	
EXTERNAL_DETAILS_URL	
SOURCE_MEMBERS	1552364_s_at,1552365_at,1552378_s_at,1552715_a_at,1552790_a_at,1552978_a_at,1553994_at,1553995_a_at,1554479_a_at,1555125_at,1555526_a_at,1555929_s_at,1555950_a_at,1556035_s_at,1556051_a_at,1557383_a_at,1558080_s_at,1558365_at,1558747_at,1559477_s_at,1559535_s_at,1560486_at,1560589_a_at,1561939_at,1562283_at,1564158_a_at,1564248_at,200632_s_at,200644_at,200732_s_at,200832_s_at,200884_at,200904_at,200920_s_at,200921_s_at,200962_at,201070_x_at,201131_s_at,201169_s_at,201170_s_at,201185_at,201294_s_at,201310_s_at,201427_s_at,201432_at,201473_at,201505_at,201641_at,201694_s_at,201695_s_at,201829_at,201834_at,201917_s_at,201918_at,202022_at,202081_at,202214_s_at,202331_at,202364_at,202411_at,202464_s_at,202481_at,202554_s_at,202619_s_at,202688_at,202731_at,202833_s_at,202887_s_at,202905_x_at,202912_at,202934_at,203021_at,203060_s_at,203080_s_at,203282_at,203293_s_at,203348_s_at,203355_s_at,203395_s_at,203397_s_at,203642_s_at,203661_s_at,203662_s_at,203688_at,203716_s_at,203762_s_at,203780_at,203791_at,203799_at,203887_s_at,203911_at,203917_at,203939_at,203962_s_at,203963_at,204011_at,204069_at,204158_s_at,204225_at,204259_at,204475_at,204508_s_at,204532_x_at,204595_s_at,204596_s_at,204597_x_at,204613_at,204697_s_at,204698_at,204742_s_at,204820_s_at,204821_at,204981_at,204985_s_at,205198_s_at,205221_at,205225_at,205343_at,205489_at,205583_s_at,205593_s_at,205670_at,205787_x_at,205799_s_at,205880_at,205952_at,206058_at,206204_at,206332_s_at,206662_at,206667_s_at,206668_s_at,206831_s_at,206893_at,207016_s_at,207076_s_at,207186_s_at,207414_s_at,207480_s_at,207528_s_at,207543_s_at,207808_s_at,208073_x_at,208498_s_at,208596_s_at,208661_s_at,208662_s_at,208663_s_at,208763_s_at,208797_s_at,208859_s_at,208891_at,208933_s_at,208998_at,209108_at,209114_at,209189_at,209373_at,209453_at,209457_at,209655_s_at,209782_s_at,209822_s_at,209846_s_at,209875_s_at,209935_at,209937_at,210029_at,210111_s_at,210130_s_at,210172_at,210377_at,210425_x_at,210513_s_at,210592_s_at,210664_s_at,210735_s_at,210761_s_at,210858_x_at,211000_s_at,211074_at,211458_s_at,211470_s_at,211527_x_at,211685_s_at,211922_s_at,211998_at,212007_at,212171_x_at,212179_at,212249_at,212268_at,212343_at,212417_at,212425_at,212442_s_at,212451_at,212492_s_at,212496_s_at,212510_at,212531_at,212543_at,212558_at,212593_s_at,212774_at,212805_at,212820_at,212831_at,212899_at,212907_at,213015_at,213156_at,213288_at,213353_at,213355_at,213391_at,213557_at,213572_s_at,213624_at,213649_at,213664_at,213686_at,213693_s_at,213750_at,213816_s_at,213939_s_at,213988_s_at,213997_at,214022_s_at,214218_s_at,214290_s_at,214305_s_at,214308_s_at,214329_x_at,214723_x_at,214748_at,214806_at,215046_at,215086_at,215813_s_at,215867_x_at,216375_s_at,217047_s_at,217287_s_at,217478_s_at,217591_at,217707_x_at,217783_s_at,217862_at,217914_at,217984_at,217997_at,217999_s_at,218035_s_at,218164_at,218166_s_at,218280_x_at,218285_s_at,218303_x_at,218309_at,218613_at,218614_at,218807_at,218840_s_at,218858_at,218924_s_at,219155_at,219250_s_at,219271_at,219468_s_at,219469_at,219580_s_at,219717_at,219876_s_at,219891_at,220147_s_at,220468_at,220937_s_at,220940_at,220942_x_at,221024_s_at,221088_s_at,221523_s_at,221524_s_at,221551_x_at,221577_x_at,221689_s_at,221702_s_at,221728_x_at,221953_s_at,221985_at,221986_s_at,222111_at,222125_s_at,222557_at,222585_x_at,222688_at,222771_s_at,222787_s_at,222798_at,222853_at,223038_s_at,223044_at,223058_at,223134_at,223178_s_at,223217_s_at,223218_s_at,223235_s_at,223698_at,223754_at,223796_at,223839_s_at,224345_x_at,224558_s_at,224559_at,224569_s_at,224572_s_at,224588_at,224590_at,224646_x_at,224676_at,224694_at,224726_at,224755_at,224767_at,224772_at,224817_at,224876_at,224917_at,225008_at,225173_at,225237_s_at,225239_at,225240_s_at,225327_at,225391_at,225496_s_at,225517_at,225546_at,225553_at,225613_at,225731_at,225811_at,225814_at,225817_at,225842_at,225910_at,225912_at,226039_at,226090_x_at,226121_at,226134_s_at,226157_at,226158_at,226160_at,226247_at,226258_at,226312_at,226347_at,226348_at,226419_s_at,226438_at,226452_at,226512_at,226529_at,226533_at,226550_at,226552_at,226559_at,226587_at,226671_at,226728_at,226779_at,226843_s_at,226863_at,227045_at,227062_at,227082_at,227152_at,227190_at,227223_at,227226_at,227227_at,227277_at,227404_s_at,227410_at,227467_at,227525_at,227533_at,227611_at,227620_at,227671_at,227677_at,227708_at,227748_at,227776_at,228006_at,228157_at,228195_at,228483_s_at,228486_at,228494_at,228696_at,228726_at,228739_at,228927_at,228959_at,228971_at,229163_at,229269_x_at,229308_at,229342_at,229378_at,229614_at,229625_at,229775_s_at,229942_at,229943_at,230085_at,230333_at,230403_at,230435_at,230494_at,230573_at,230609_at,230624_at,230630_at,230746_s_at,230872_s_at,230918_at,231007_at,231235_at,231251_at,231332_at,231399_at,231697_s_at,231804_at,231960_at,232008_s_at,232080_at,232182_at,232794_at,232914_s_at,233169_at,233337_s_at,233388_at,233461_x_at,233543_s_at,233677_at,233914_s_at,234725_s_at,234989_at,235019_at,235051_at,235058_at,235067_at,235125_x_at,235369_at,235381_at,235433_at,235470_at,235473_at,235687_at,235709_at,235716_at,235846_at,236098_at,236254_at,236356_at,236600_at,236655_at,236773_at,236778_at,236907_at,237741_at,237953_at,238077_at,238206_at,238320_at,238363_at,238448_at,238563_at,238581_at,238649_at,238651_at,238681_at,238751_at,238846_at,238884_at,239050_s_at,239586_at,239784_at,239798_at,239808_at,240062_at,240175_at,240282_at,240304_s_at,240331_at,240991_at,241607_at,241704_x_at,241722_x_at,241827_at,241843_at,242052_at,242134_at,242343_x_at,242518_at,242750_at,242853_at,242919_at,242969_at,243134_at,243252_at,243264_s_at,243386_at,243462_s_at,243509_at,243751_at,243768_at,243802_at,244008_at,244065_at,244181_at,244447_at,36553_at,36711_at,38241_at,40569_at,43511_s_at,44783_s_at,49111_at
GENE_SYMBOLS	MSI2,SCIN,RDH10,RXFP1,SEC62,SCAMP1,NT5E,NT5E,CARD8,PAXBP1,SEPTIN6,CNTNAP3,CD55,ZNF207,BICD1,,DNAJC3,PGK1,SMCHD1,MEIS1,MRNIP-DT,FNDC7,LINC02683,DYNC2H1,,VAC14-AS1,,NDRG1,MARCKSL1,PTP4A1,SCD,CKB,HLA-E,BTG1,BTG1,RPL31,SF3B1,CDH1,BHLHE40,BHLHE40,HTRA1,WSB1,NREP,SELENOP,CAT,JUNB,LAMB1,BST2,EGR1,PNP,NET1,PRKAB1,SLC25A36,SLC25A36,ALDOC,IER2,CUL4B,BCKDHA,MXI1,IFI27,PFKFB3,DHRS3,GSTM3,PLOD2,TNFSF10,PDCD4,SERPINA1,DDIT4,NBN,ADM,HK2,SLPI,PAPSS2,BAZ2B,GBE1,LMAN1,ETV5,PSD3,HES1,GALNT3,COBLL1,TMOD1,TMOD1,PKD2,DPP4,DYNC2LI1,MPZL2,DMXL1,CD302,THBD,RAP1GAP,CXADR,NT5E,NEBL,CA12,SPRY2,MEIS1,TCIRG1,HDAC4,MMP7,MMP1,CA12,UGT1A10,STC1,STC1,STC1,PLCG2,CHGA,ISG20,PDS5B,BTN3A3,BTN3A3,SLC67A1,TRAPPC6A,ATP7A,HGD,ESR1,SULT1C2,CRYM,ALG13,PDE9A,GAL3ST1,ZC3H11A,SLC3A1,PRKD1,KCNK3,SLC6A12,GRB14,IFI16,GLRX,SCAMP1,SCAMP1,ARSD,SALL1,ALDH1A2,ASS1,BPTF,PCSK6,MEIS2,SLC7A11,P4HA1,PROS1,TTC3,AMY1A,UGT1A10,TTC3,TTC3,TTC3,TSC22D3,GOLGA8A,ATRX,DUSP6,LGALS8,UCP2,TSPAN6,TSPAN1,FOS,MALL,SLC9A1,DUSP5,TMEM47,DBP,VLDLR,BTN3A2,SPP1,ATP2C1,TM4SF4,IDO1,KLHDC10,TM7SF2,SF1,ACSM3,GOLGA8A,VEGFA,SAT1,TFPI,CA12,GRB7,ATM,IL6ST,,GABARAPL1,SULT1C2,VEGFA,NCALD,CAT,H3-3B,UBXN4,VEGFA,PNISR,PIK3R1,SERPINB1,YIPF6,SCAMP1,SCAMP1,CERS6,SECISBP2L,KDM4B,KDM4B,GPD1L,LCN2,CRYBG1,SPRY1,PDCD4,ZBTB18,PRUNE2,DMXL2,MEGF9,CDK19,SLC30A1,BBX,ZBTB20,MBOAT2,ABCA5,ST3GAL6,DPY19L4,CDK12,SERPINB1,SMPDL3A,SRSF7,SLC1A1,VPS13A,MUC1,RSL1D1,MET,RUFY3,SAT1,ENTREP2,IFITM1,XIST,H2AC18,SF3B1,HGD,TNFSF10,ANKRD36B,N4BP2L2,BICD1,KANSL1L,,PTGS1,CA12,ETV5,FAM13A,TRPC6,HLA-DMA,SKIL,,YPEL5,PIAS1,TPCN1,RNASET2,NAP1L1,NAP1L1,RBM47,SPATA20,RSF1,H2AC18,BDH2,KRCC1,CAMK2N1,PSD3,RESF1,VAV3,NADSYN1,DEPTOR,CTBS,PITPNC1,FLRT3,GALNT14,CUEDC1,DYNC2H1,TMC5,DCAF16,GOLGA2P5,PGPEP1,SINHCAF,ARL14,ST6GALNAC4,,FAM162A,SLC2A10,PPP1R9A,RRAGD,RRAGD,ST6GALNAC4,GDF15,PIGP,TM2D3,XIST,MMP24OS,KLHL24,KLHL24,MINDY2,P4HTM,STMN3,KRCC1,ACER3,MYEF2,TMEM106B,PTER,FLRT3,SINHCAF,SLC40A1,FAM107B,BBX,NT5DC1,NFKBIZ,NFKBIZ,SMOC2,SLC25A36,C2orf88,CNTNAP3,,FAM162A,MALAT1,MALAT1,IRF2BP2,IRF2BP2,XIST,XIST,H19,TMED4,ANTXR1,MIB1,TM9SF3,RPL37,NAV1,SH3PXD2A,C5orf24,VMP1,ASPH,ARHGAP18,MSI2,NEAT1,MSI2,ATOSA,MRFAP1L2,SYTL2,ZNF770,EEF2K,CNIH1,MAST4,ANKRD50,C11orf58,XRN1,CGNL1,NAP1L1,HELZ,TP53INP1,MGAT4A,RABL3,DHRS13,MSI2,TFDP2,KLHL24,H6PD,PLEKHA1,AMN1,RICTOR,,FUT11,,SNTB1,PDK1,ZMYM2,TMEM106B,HINT3,SLC9A7,IER5L,IER5L,SNHG14,LAMP2,SLC27A1,LMBRD2,TENT4B,FAM110C,ZNF614,NEAT1,ZBTB20,RESF1,TMEM37,RBM39,MRAP2,ARL17B,MTDH,,FAM43A,RDH10,GLCCI1,RALGPS2,TARS3,SLC44A1,XIST,JAK3,EEF1A1,RBMXL1,ACER3,PTEN,ZNF207,C2orf88,PGK1,SLC44A1,PPP1R9A,SLC45A3,SERPINB1,CYS1,ZNF397,PDK3,SLC25A3,,SSBP4,ANKRD29,NCBP3,STOX1,ZNF320,GBP5,,BNC2,TRIM13,PDK3,,RFX3,FAM228B,SLC20A1,SGK2,CLINT1,SLC25A27,AK4,,TTLL3,GALK2,,NKTR,WIPF2,,RAB3IP,,RXFP1,BRWD1,BBX,HECW2,FRG1HP,,SYTL2,ZNF350,SEZ6L2,,ZNF226,ABRAXAS1,,SBF2,SEMA4B,NEAT1,CPM,CCDC50,GPN2,MKLN1,MIGA1,DORIP1,RSF1,APOOL,LSM8,MED6,ZNF626,GAS2L3,,FSBP,ERLN,VPS13B,NDUFS1,SPART,TPD52,,,PABPC1,SLC25A36,,KCTD6,RXFP1,NEAT1,,MRPL19,,GBP5,PITPNC1,,GDPD1,SORBS2,TNFRSF11A,,CRIPT,FAM83A,,PDK1,,FAM3C,KCNK5,WDR1,TMC5,,,CRYZL2P,ZNF320,MCL1,ZNF615,EIF5,,,,,MMAA,,ZNF253,ZNF780B,,FRRS1,C8orf44,CASZ1,,,CHD2,RNU6-1016P,DNAH12,PARP8,CNTNAP3,,,ASMTL,MAFF,BTN3A3,MZF1,ARRB1,HEY1,ARRB1
FOUNDER_NAMES