STANDARD_NAME	CASTELLANO_NRAS_TARGETS_DN
SYSTEMATIC_NAME	M12848
COLLECTION	C2:CGP
MSIGDB_URL	https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/CASTELLANO_NRAS_TARGETS_DN
NAMESPACE	AFFY_MG_U74
DESCRIPTION_BRIEF	Genes down-regulated in MEF cells (embryonic fibroblast) isolated from NRAS [GeneID=4893] knockout mice.
DESCRIPTION_FULL	We characterized differential gene expression profiles of fibroblast cell lines harboring single or double-homozygous null mutations in H-ras and N-ras. Whereas the expression level of the individual H-, N- and K-ras genes appeared unaffected by the presence or absence of the other ras loci, significant differences were observed between the expression profiles of cells missing N-ras and/or H-ras. Absence of N-ras produced much stronger effects than absence of H-ras over the profile of the cellular transcriptome. N-ras(-/-) and H-ras(-/-) fibroblasts displayed rather antagonistic expression profiles and the transcriptome of H-ras(-/-) cells was significantly closer to that of wild-type fibroblasts than to that of N-ras(-/-) cells. Classifying all differentially expressed genes into functional categories suggested specific roles for H-Ras and N-Ras. It was particularly striking in N-ras(-/-) cells the upregulation of a remarkable number of immunity-related genes, as well as of several loci involved in apoptosis. Reverse-phase protein array assays demonstrated in the same N-ras(-/-) cells the overexpression and nuclear migration of tyrosine phosphorylated signal transducer and activator of transcription 1 (Stat1) which was concomitant with transcriptional activation mediated by interferon-stimulated response elements. Significantly enhanced numbers of apoptotic cells were also detected in cultures of N-ras(-/-) cells. Our data support the notion that different Ras isoforms play functionally distinct cellular roles and indicate that N-Ras is significantly involved in immune modulation/host defense and apoptotic responses.
PMID	16909116
GEOID	
AUTHORS	Castellano E,De Las Rivas J,Guerrero C,Santos E
CONTRIBUTOR	Leona Saunders
CONTRIBUTOR_ORG	MSigDB Team
EXACT_SOURCE	Table 2: delta(i) < 0
FILTERED_BY_SIMILARITY	
EXTERNAL_NAMES_FOR_SIMILAR_TERMS	
EXTERNAL_DETAILS_URL	
SOURCE_MEMBERS	100153_at,100928_at,102048_at,102399_at,102888_s_at,103467_g_at,103888_at,160925_at,94362_at,95022_at,95150_at,96920_at,98005_at,98007_at,98624_at,98789_at
GENE_SYMBOLS	NCAM1,FBLN2,ANKRD1,RBPMS,LIMK1,ZFTRAF1,RBPMS,NRAS,NRAS,AKAP12,PLEKHO1,HTRA1,PKIA,RPS6KA2,RBM38,CDKN2A
FOUNDER_NAMES