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<MSIGDB BUILD_DATE="Jan 29, 2026" VERSION="2026.1.Hs" NAME="export">
    <GENESET STANDARD_NAME="CASTELLANO_NRAS_TARGETS_DN" SYSTEMATIC_NAME="M12848" HISTORICAL_NAMES="" PMID="16909116" AUTHORS="Castellano E,De Las Rivas J,Guerrero C,Santos E" GEOID="" EXACT_SOURCE="Table 2: delta(i) &lt; 0" GENESET_LISTING_URL="" EXTERNAL_DETAILS_URL="" CHIP="AFFY_MG_U74" CONTRIBUTOR="Leona Saunders" CONTRIBUTOR_ORG="MSigDB Team" DESCRIPTION_BRIEF="Genes down-regulated in MEF cells (embryonic fibroblast) isolated from NRAS [GeneID=4893] knockout mice." DESCRIPTION_FULL="We characterized differential gene expression profiles of fibroblast cell lines harboring single or double-homozygous null mutations in H-ras and N-ras. Whereas the expression level of the individual H-, N- and K-ras genes appeared unaffected by the presence or absence of the other ras loci, significant differences were observed between the expression profiles of cells missing N-ras and/or H-ras. Absence of N-ras produced much stronger effects than absence of H-ras over the profile of the cellular transcriptome. N-ras(-/-) and H-ras(-/-) fibroblasts displayed rather antagonistic expression profiles and the transcriptome of H-ras(-/-) cells was significantly closer to that of wild-type fibroblasts than to that of N-ras(-/-) cells. Classifying all differentially expressed genes into functional categories suggested specific roles for H-Ras and N-Ras. It was particularly striking in N-ras(-/-) cells the upregulation of a remarkable number of immunity-related genes, as well as of several loci involved in apoptosis. Reverse-phase protein array assays demonstrated in the same N-ras(-/-) cells the overexpression and nuclear migration of tyrosine phosphorylated signal transducer and activator of transcription 1 (Stat1) which was concomitant with transcriptional activation mediated by interferon-stimulated response elements. Significantly enhanced numbers of apoptotic cells were also detected in cultures of N-ras(-/-) cells. Our data support the notion that different Ras isoforms play functionally distinct cellular roles and indicate that N-Ras is significantly involved in immune modulation/host defense and apoptotic responses." TAGS="" MEMBERS="100153_at,100928_at,102048_at,102399_at,102888_s_at,103467_g_at,103888_at,160925_at,94362_at,95022_at,95150_at,96920_at,98005_at,98007_at,98624_at,98789_at" MEMBERS_SYMBOLIZED="AKAP12,ANKRD1,CDKN2A,FBLN2,HTRA1,LIMK1,NCAM1,NRAS,PKIA,PLEKHO1,RBM38,RBPMS,RPS6KA2,ZFTRAF1" MEMBERS_EZID="1029,11030,2199,27063,3984,4684,4893,50626,51177,55544,5569,5654,6196,9590" MEMBERS_MAPPING="100153_at,NCAM1,4684|100928_at,FBLN2,2199|102048_at,ANKRD1,27063|102399_at,RBPMS,11030|102888_s_at,LIMK1,3984|103467_g_at,ZFTRAF1,50626|103888_at,RBPMS,11030|160925_at,NRAS,4893|94362_at,NRAS,4893|95022_at,AKAP12,9590|95150_at,PLEKHO1,51177|96920_at,HTRA1,5654|98005_at,PKIA,5569|98007_at,RPS6KA2,6196|98624_at,RBM38,55544|98789_at,CDKN2A,1029" FILTERED_BY_SIMILARITY="" FOUNDER_NAMES="" REFINEMENT_DATASETS="" VALIDATION_DATASETS="" CATEGORY_CODE="C2" ORGANISM="Mus musculus" SUB_CATEGORY_CODE="CGP"/>
</MSIGDB>
