STANDARD_NAME CASTELLANO_NRAS_TARGETS_UP SYSTEMATIC_NAME M18168 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/CASTELLANO_NRAS_TARGETS_UP NAMESPACE AFFY_MG_U74 DESCRIPTION_BRIEF Genes up-regulated in MEF cells (embryonic fibroblast) isolated from NRAS [GeneID=4893] knockout mice. DESCRIPTION_FULL We characterized differential gene expression profiles of fibroblast cell lines harboring single or double-homozygous null mutations in H-ras and N-ras. Whereas the expression level of the individual H-, N- and K-ras genes appeared unaffected by the presence or absence of the other ras loci, significant differences were observed between the expression profiles of cells missing N-ras and/or H-ras. Absence of N-ras produced much stronger effects than absence of H-ras over the profile of the cellular transcriptome. N-ras(-/-) and H-ras(-/-) fibroblasts displayed rather antagonistic expression profiles and the transcriptome of H-ras(-/-) cells was significantly closer to that of wild-type fibroblasts than to that of N-ras(-/-) cells. Classifying all differentially expressed genes into functional categories suggested specific roles for H-Ras and N-Ras. It was particularly striking in N-ras(-/-) cells the upregulation of a remarkable number of immunity-related genes, as well as of several loci involved in apoptosis. Reverse-phase protein array assays demonstrated in the same N-ras(-/-) cells the overexpression and nuclear migration of tyrosine phosphorylated signal transducer and activator of transcription 1 (Stat1) which was concomitant with transcriptional activation mediated by interferon-stimulated response elements. Significantly enhanced numbers of apoptotic cells were also detected in cultures of N-ras(-/-) cells. Our data support the notion that different Ras isoforms play functionally distinct cellular roles and indicate that N-Ras is significantly involved in immune modulation/host defense and apoptotic responses. PMID 16909116 GEOID AUTHORS Castellano E,De Las Rivas J,Guerrero C,Santos E CONTRIBUTOR Leona Saunders CONTRIBUTOR_ORG MSigDB Team EXACT_SOURCE Table 2: delta(i) > 0 FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS 100013_at,100065_r_at,100127_at,100471_at,100484_at,100584_at,100717_at,100927_at,100981_at,101099_at,101465_at,101587_at,101593_at,101853_f_at,101886_f_at,102053_at,102373_at,102791_at,102906_at,103035_at,103241_at,103244_at,103443_at,103446_at,103483_at,103723_at,103963_f_at,104093_at,104165_at,104237_at,104361_at,104419_at,104519_at,104597_at,104735_at,160127_at,160564_at,160775_at,160799_at,160989_r_at,161030_at,161173_f_at,161511_f_at,92581_at,92607_at,92718_at,92731_at,92930_at,93088_at,93321_at,93466_at,93536_at,93714_f_at,93860_i_at,93861_f_at,93866_s_at,93871_at,93940_at,94021_at,94350_f_at,94516_f_at,94774_at,94835_f_at,95024_at,95480_at,95523_at,96033_at,96123_at,96763_at,96764_at,96801_at,97374_at,97386_at,97409_at,97487_at,97529_at,97825_at,97979_at,98008_at,98056_at,98438_f_at,98465_f_at,98466_r_at,98633_at,98822_at,99366_at,99580_s_at,99675_at GENE_SYMBOLS IFI35,GJA1,CRABP2,P3H4,MMP13,ANXA4,,PLTP,IFIT1B,NSG1,STAT1,EPHX1,CRIP2,CFH,HLA-B,PLSCR1,ENPEP,PSMB8,,TAP1,ADCY2,CLEC11A,CRYBG1,IFIH1,ERCC5,IL13RA1,,LSP1,VNN1,PCBD2,ZNF32,FNDC3A,ITIH2,GBP2,KCTD12,CCNG1,LCN2,TULP4,,NPR3,SCX,,,ACADM,MEST,IFI27L2,PTX3,DLX5,B2M,IFI16,EXOC4,BAX,,,,MGP,IL1RN,PON3,TRIM11,NQO1,PENK,,TUBB2A,USP18,RAMP2,,SDC1,LBP,SARDH,,AK1,,ZNF703,IRGM,SERPINE2,ANXA8L1,PERP,PPP1R7,CX3CL1,PHLDA3,,IFI16,IFI16,TMEM43,ISG15,SLC66A3,UGT1A10,YIF1A FOUNDER_NAMES