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<MSIGDB BUILD_DATE="Jan 29, 2026" VERSION="2026.1.Hs" NAME="export">
    <GENESET STANDARD_NAME="CASTELLANO_NRAS_TARGETS_UP" SYSTEMATIC_NAME="M18168" HISTORICAL_NAMES="" PMID="16909116" AUTHORS="Castellano E,De Las Rivas J,Guerrero C,Santos E" GEOID="" EXACT_SOURCE="Table 2: delta(i) &gt; 0" GENESET_LISTING_URL="" EXTERNAL_DETAILS_URL="" CHIP="AFFY_MG_U74" CONTRIBUTOR="Leona Saunders" CONTRIBUTOR_ORG="MSigDB Team" DESCRIPTION_BRIEF="Genes up-regulated in MEF cells (embryonic fibroblast) isolated from NRAS [GeneID=4893] knockout mice." DESCRIPTION_FULL="We characterized differential gene expression profiles of fibroblast cell lines harboring single or double-homozygous null mutations in H-ras and N-ras. Whereas the expression level of the individual H-, N- and K-ras genes appeared unaffected by the presence or absence of the other ras loci, significant differences were observed between the expression profiles of cells missing N-ras and/or H-ras. Absence of N-ras produced much stronger effects than absence of H-ras over the profile of the cellular transcriptome. N-ras(-/-) and H-ras(-/-) fibroblasts displayed rather antagonistic expression profiles and the transcriptome of H-ras(-/-) cells was significantly closer to that of wild-type fibroblasts than to that of N-ras(-/-) cells. Classifying all differentially expressed genes into functional categories suggested specific roles for H-Ras and N-Ras. It was particularly striking in N-ras(-/-) cells the upregulation of a remarkable number of immunity-related genes, as well as of several loci involved in apoptosis. Reverse-phase protein array assays demonstrated in the same N-ras(-/-) cells the overexpression and nuclear migration of tyrosine phosphorylated signal transducer and activator of transcription 1 (Stat1) which was concomitant with transcriptional activation mediated by interferon-stimulated response elements. Significantly enhanced numbers of apoptotic cells were also detected in cultures of N-ras(-/-) cells. Our data support the notion that different Ras isoforms play functionally distinct cellular roles and indicate that N-Ras is significantly involved in immune modulation/host defense and apoptotic responses." TAGS="" MEMBERS="100013_at,100065_r_at,100127_at,100471_at,100484_at,100584_at,100717_at,100927_at,100981_at,101099_at,101465_at,101587_at,101593_at,101853_f_at,101886_f_at,102053_at,102373_at,102791_at,102906_at,103035_at,103241_at,103244_at,103443_at,103446_at,103483_at,103723_at,103963_f_at,104093_at,104165_at,104237_at,104361_at,104419_at,104519_at,104597_at,104735_at,160127_at,160564_at,160775_at,160799_at,160989_r_at,161030_at,161173_f_at,161511_f_at,92581_at,92607_at,92718_at,92731_at,92930_at,93088_at,93321_at,93466_at,93536_at,93714_f_at,93860_i_at,93861_f_at,93866_s_at,93871_at,93940_at,94021_at,94350_f_at,94516_f_at,94774_at,94835_f_at,95024_at,95480_at,95523_at,96033_at,96123_at,96763_at,96764_at,96801_at,97374_at,97386_at,97409_at,97487_at,97529_at,97825_at,97979_at,98008_at,98056_at,98438_f_at,98465_f_at,98466_r_at,98633_at,98822_at,99366_at,99580_s_at,99675_at" MEMBERS_SYMBOLIZED="ACADM,ADCY2,AK1,ANXA4,ANXA8L1,B2M,BAX,CCNG1,CFH,CLEC11A,CRABP2,CRIP2,CRYBG1,CX3CL1,DLX5,ENPEP,EPHX1,ERCC5,EXOC4,FNDC3A,GBP2,GJA1,HLA-B,IFI27L2,IFI35,IFIH1,IFIT1B,IL13RA1,IL1RN,IRGM,ISG15,ITIH2,KCTD12,LBP,LCN2,LSP1,MEST,MGP,MMP13,MNDA,NPR3,NQO1,NSG1,P3H4,PCBD2,PENK,PERP,PHLDA3,PLSCR1,PLTP,PON3,PPP1R7,PSMB8,PTX3,RAMP2,SARDH,SCX,SDC1,SERPINE2,SLC66A3,STAT1,TAP1,TMEM43,TRIM11,TUBB2A,TULP4,UGT1A10,USP18,VNN1,YIF1A,ZNF32,ZNF703" MEMBERS_EZID="10266,10609,108,10897,11274,115207,130814,1382,1397,1728,1749,1757,202,2028,203,2052,2073,22862,23612,2634,2697,27065,307,3075,3106,34,3430,345611,3557,3597,3698,3929,3934,4046,4232,4256,4322,4332,439996,4883,5179,5270,5359,5360,5446,54575,5510,567,5696,56995,5806,581,60412,6320,6376,6382,64065,64135,642658,6772,6890,7280,728113,7580,79188,80139,81559,83982,84105,8876,900,9636" MEMBERS_MAPPING="100013_at,IFI35,3430|100065_r_at,GJA1,2697|100127_at,CRABP2,1382|100471_at,P3H4,10609|100484_at,MMP13,4322|100584_at,ANXA4,307|100717_at,null,null|100927_at,PLTP,5360|100981_at,IFIT1B,439996|101099_at,NSG1,27065|101465_at,STAT1,6772|101587_at,EPHX1,2052|101593_at,CRIP2,1397|101853_f_at,CFH,3075|101886_f_at,HLA-B,3106|102053_at,PLSCR1,5359|102373_at,ENPEP,2028|102791_at,PSMB8,5696|102906_at,null,null|103035_at,TAP1,6890|103241_at,ADCY2,108|103244_at,CLEC11A,6320|103443_at,CRYBG1,202|103446_at,IFIH1,64135|103483_at,ERCC5,2073|103723_at,IL13RA1,3597|103963_f_at,null,null|104093_at,LSP1,4046|104165_at,VNN1,8876|104237_at,PCBD2,84105|104361_at,ZNF32,7580|104419_at,FNDC3A,22862|104519_at,ITIH2,3698|104597_at,GBP2,2634|104735_at,KCTD12,115207|160127_at,CCNG1,900|160564_at,LCN2,3934|160775_at,TULP4,56995|160799_at,null,null|160989_r_at,NPR3,4883|161030_at,SCX,642658|161173_f_at,null,null|161511_f_at,null,null|92581_at,ACADM,34|92607_at,MEST,4232|92718_at,IFI27L2,83982|92731_at,PTX3,5806|92930_at,DLX5,1749|93088_at,B2M,567|93321_at,null,null|93466_at,EXOC4,60412|93536_at,BAX,581|93714_f_at,null,null|93860_i_at,null,null|93861_f_at,null,null|93866_s_at,MGP,4256|93871_at,IL1RN,3557|93940_at,PON3,5446|94021_at,TRIM11,81559|94350_f_at,NQO1,1728|94516_f_at,PENK,5179|94774_at,null,null|94835_f_at,TUBB2A,7280|95024_at,USP18,11274|95480_at,RAMP2,10266|95523_at,null,null|96033_at,SDC1,6382|96123_at,LBP,3929|96763_at,SARDH,1757|96764_at,null,null|96801_at,AK1,203|97374_at,null,null|97386_at,ZNF703,80139|97409_at,IRGM,345611|97487_at,SERPINE2,5270|97529_at,ANXA8L1,728113|97825_at,PERP,64065|97979_at,PPP1R7,5510|98008_at,CX3CL1,6376|98056_at,PHLDA3,23612|98438_f_at,HLA-B,3106|98465_f_at,MNDA,4332|98466_r_at,MNDA,4332|98633_at,TMEM43,79188|98822_at,ISG15,9636|99366_at,SLC66A3,130814|99580_s_at,UGT1A10,54575|99675_at,YIF1A,10897" FILTERED_BY_SIMILARITY="" FOUNDER_NAMES="" REFINEMENT_DATASETS="" VALIDATION_DATASETS="" CATEGORY_CODE="C2" ORGANISM="Mus musculus" SUB_CATEGORY_CODE="CGP"/>
</MSIGDB>
