STANDARD_NAME CHANGOLKAR_H2AFY_TARGETS_DN SYSTEMATIC_NAME M2290 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/CHANGOLKAR_H2AFY_TARGETS_DN NAMESPACE AFFY_Mouse430 DESCRIPTION_BRIEF Genes down-regulated in liver tissue upon knockout of H2AFY [GeneID=9555]. DESCRIPTION_FULL macroH2A histone variants have been implicated to function in gene silencing by several studies, including ones showing a preferential association of macroH2A on the inactive X chromosome. To examine macroH2A function in vivo, we knocked out macroH2A1. macroH2A1 knockout mice are viable and fertile. A broad screen of liver gene expression showed no evidence of defects in X inactivation but did identify genes that have increased expression levels in macroH2A1 knockouts. macroH2A1-containing nucleosomes are enriched on the coding and/or upstream regions of these genes, suggesting that their increased expression levels are a direct effect of the absence of macroH2A1. The concentrations of macroH2A1 nucleosomes on these genes are low in the livers of newborn mice, and the macroH2A1 knockout had little effect on the expression levels of these genes in newborn liver. Our results indicate that an increase in liver macroH2A1 during the transition from newborn to young-adult status contributes to a decrease in the expression levels of these genes. These genes cluster in the area of lipid metabolism, and we observed metabolic effects in macroH2A1 knockouts. Our results indicate that the function of macroH2A1 histones is not restricted to gene silencing but also involves fine tuning the expression of specific genes. PMID 17242180 GEOID AUTHORS Changolkar LN,Costanzi C,Leu NA,Chen D,McLaughlin KJ,Pehrson JR CONTRIBUTOR Arthur Liberzon CONTRIBUTOR_ORG MSigDB Team EXACT_SOURCE Table 1S: ratio ko/cont < 1 FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS 1416795_at,1417242_at,1417962_s_at,1418388_s_at,1418804_at,1419393_at,1419611_at,1419622_at,1419677_at,1421456_at,1422072_a_at,1423316_at,1423512_at,1423859_a_at,1423884_at,1424133_at,1424572_a_at,1425506_at,1426269_at,1426773_at,1427411_s_at,1428496_at,1431566_at,1434330_at,1434481_at,1435800_a_at,1436346_at,1436766_at,1436767_at,1436881_x_at,1436885_a_at,1436972_at,1437064_at,1437197_at,1437761_at,1437894_at,1441592_at,1442351_a_at,1449465_at,1449861_at,1449870_a_at,1450531_at,1450744_at,1450873_at,1451625_a_at,1452007_at,1455208_at,1455643_s_at,1456607_at,1456887_at,1457362_at,1459488_at,1460228_at GENE_SYMBOLS CRYL1,EIF4A3,GHR,MPHOSPH8,SUCNR1,ABCG5,,UGT2B28,MASP1,P2RY1,GSTM1,TMEM39A,C7orf25,PTGDS,UTP4,TMEM98,MACROH2A1,MYLK,,MFN1,,SECISBP2,LINC00261,TBCEL,MSL1,YBX3,CD109,LUC7L2,,,CHERP,GPR61,AR,SORBS2,LUC7L2,PROX1,,PAXX,RELN,NEK4,ATP6V0A2,,ELL2,GTPBP4,C8G,,PEX19,TSR1,VCPIP1,CMKLR1,,,USF2 FOUNDER_NAMES