STANDARD_NAME CHEBOTAEV_GR_TARGETS_DN SYSTEMATIC_NAME M7876 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/CHEBOTAEV_GR_TARGETS_DN NAMESPACE AFFY_Mouse430 DESCRIPTION_BRIEF Genes down-regulated in follicular epithelial stem cells after transgenic expression of GR [GeneID=2908] under control of the keratin5 (K5) [GeneID=3852] promoter. DESCRIPTION_FULL Glucocorticoids are potent inhibitors of mouse skin tumorigenesis. The glucocorticoid control of cellular functions is mediated via the glucocorticoid receptor (GR), a well-known transcription factor. Recently, we generated transgenic mice overexpressing GR under control of the keratin5 (K5) promoter, and showed that K5.GR animals are resistant to skin carcinogenesis. Follicular epithelial stem cells (SCs), located in the bulge region of the hair follicle, are believed to be one of the target cells for skin carcinogenesis. We found that the number of putative hair follicle SC detected as label-retaining cells was significantly less in the K5.GR transgenics compared to wild type (w.t.) littermates. We also showed that GR overexpression led to a reduction in the clonogenicity of the follicular epithelial SCs. We evaluated the global effect of GR on gene expression in a population of follicular SC-enriched bulge keratinocytes isolated by fluorescence activated cell sorting. We found that GR affected the expression of numerous bulge SC 'signature' genes, genes involved in the maintenance of SC and progenitor cells of non-epidermal origin and proapoptotic genes. Our findings underscore the important role of GR signaling in the homeostasis of follicular epithelial SCs, and suggest that the reduction in their number may underlie the tumor suppressor effect of GR in the skin. PMID 17146443 GEOID AUTHORS Chebotaev D,Yemelyanov A,Zhu L,Lavker RM,Budunova I CONTRIBUTOR Leona Saunders CONTRIBUTOR_ORG MSigDB Team EXACT_SOURCE Table 2S FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS 1415806_at,1415850_at,1415949_at,1415951_at,1416051_at,1416295_a_at,1416473_a_at,1416474_at,1416905_at,1417018_at,1417122_at,1417574_at,1417765_a_at,1417849_at,1417960_at,1418191_at,1418209_a_at,1418796_at,1419056_at,1419080_at,1419490_at,1420537_at,1420859_at,1421041_s_at,1421073_a_at,1421173_at,1421497_at,1421856_at,1421882_a_at,1421883_at,1422528_a_at,1422571_at,1422673_at,1422877_at,1423062_at,1423091_a_at,1423110_at,1423250_a_at,1423258_at,1423497_at,1423597_at,1424208_at,1424531_a_at,1424542_at,1424680_at,1425110_at,1425111_at,1425350_a_at,1425742_a_at,1426288_at,1426399_at,1426872_at,1427228_at,1427308_at,1427482_a_at,1427508_at,1427655_a_at,1428111_at,1428114_at,1428493_at,1429146_at,1429284_at,1429417_at,1429824_at,1430306_a_at,1430583_at,1431398_at,1433481_at,1433577_at,1433596_at,1433647_s_at,1433691_at,1433716_x_at,1433759_at,1433768_at,1433894_at,1433965_at,1434073_at,1434380_at,1434442_at,1434539_at,1434553_at,1434709_at,1434758_at,1434877_at,1434944_at,1435106_at,1435321_at,1435462_at,1435577_at,1435578_s_at,1435649_at,1435705_at,1436082_at,1436442_at,1436838_x_at,1436912_at,1436981_a_at,1437059_at,1437064_at,1437217_at,1437449_at,1437528_x_at,1437811_x_at,1437935_at,1438193_at,1439041_at,1439300_at,1439560_x_at,1439804_at,1440589_at,1440975_at,1441783_at,1441801_at,1441912_x_at,1442077_at,1443033_at,1443281_at,1443827_x_at,1444207_at,1445913_at,1446512_at,1446771_at,1447623_s_at,1447655_x_at,1447791_s_at,1447862_x_at,1448426_at,1448558_a_at,1448600_s_at,1448823_at,1449033_at,1449178_at,1449227_at,1449632_s_at,1449848_at,1450004_at,1450227_at,1450663_at,1450922_a_at,1450923_at,1450981_at,1451835_at,1451978_at,1452114_s_at,1452312_at,1452614_at,1452670_at,1452799_at,1452981_at,1453087_at,1454301_at,1454728_s_at,1454799_at,1454862_at,1455091_at,1455493_at,1455607_at,1455647_at,1455961_at,1456137_at,1456512_at,1456533_at,1456596_at,1456874_at,1456991_at,1457664_x_at,1458114_at,1458268_s_at,1458405_at,1459847_x_at,1460229_at,1460292_a_at,1460337_at,1460606_at GENE_SYMBOLS PLAT,RASA3,CPE,FKBP10,C2,IL2RG,IGDCC4,IGDCC4,GUCA2A,EFEMP2,VAV3,CXCL12,AMY2A,ZNF704,CPEB1,USP18,PFN2,CLEC11A,RTN2,GDNF,TAFA5,KCTD4,PKIA,GSTA1,PTGER4,IRF4,GPHA2,S100A3,ELAVL2,ELAVL2,ZFP36L1,THBS2,PRKD1,PCDHB5,IGFBP3,GPM6B,COL1A2,TGFB2,SYT9,KLHL10,ATP8A1,PTGER4,TCEA3,S100A4,CALHM5,SORCS3,SORCS3,MYEF2,TSC22D1,LRP4,VWA1,FCGBP,PALLD,DAB1,CA8,ARSI,TRDC,SLC38A4,SLC14A1,SIPA1L3,SVIP,MOB3B,CHSY3,C11orf65,ATP6V1C2,NRCAM,,FKBP14,MINAR2,DNAJC6,RHOBTB3,PPP1R3C,GFRA2,DPY19L1,PALLD,JAZF1,ATP8A1,GPRASP2,GBP7,STBD1,LRRN3,TLCD4,NRCAM,CRISPLD2,NPTX1,DMPK,LIMCH1,LIMCH1,PLCXD2,DAB1,DAB1,NEXN,ZSCAN18,,PTPN14,COTL1,CACNB4,,SOX21,AR,ANKRD6,RSAD1,MINAR2,,MEI4,NRXN3,SLC39A10,CHIC1,SMIM22,,SAMD12,,,KCTD4,,HTR1B,,NRCAM,,,,,TUBA8,PRKD1,,GNA14,,SARDH,PLA2G4A,VAV3,CXCL12,TNFRSF11B,PDLIM3,CH25H,FKBP10,GNA14,TSLP,ANKRD6,THBS2,TGFB2,TGFB2,CNN2,SOX21,LOXL1,IGFBP5,,BCL2L15,MYL9,FGGY,CNTN1,,,ATP8A1,GPAT3,PHLDB2,PPP2R3A,SYNE1,RSPO3,AR,MME,NRXN3,PDZRN4,DPY19L1,TMEM255A,FLRT2,,CFB,SAMD12,IGFBP3,,,STAG3,SMARCA1,SH3KBP1,HSD17B13 FOUNDER_NAMES