STANDARD_NAME	CHEN_HOXA5_TARGETS_9HR_UP
SYSTEMATIC_NAME	M17621
COLLECTION	C2:CGP
MSIGDB_URL	https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/CHEN_HOXA5_TARGETS_9HR_UP
NAMESPACE	AFFY_HG_U133
DESCRIPTION_BRIEF	Genes up-regulated 9 h after induction of HoxA5 [GeneID=3205] expression in a breast cancer cell line.
DESCRIPTION_FULL	The homeobox gene HOXA5 encodes a transcription factor that has been shown to play important roles in embryogenesis, hematopoiesis, and tumorigenesis. In order to decipher downstream signaling pathways of HOXA5, we utilized oligonucleotide microarray analysis to identify genes that are differentially expressed in HOXA5-induced cells compared with uninduced cells. Comparative analysis of gene expression changes after 9 h of HOXA5 induction in Hs578T breast cancer cells identified 306 genes whose expression was modulated at least 2-fold. Ten of these 306 genes were also up-regulated by at least 2-fold at 6 h post-induction. The expression of all of these 10 genes was confirmed by semiquantitative reverse transcription-PCR. Among these 10 genes, which are most likely to be direct targets of HOXA5, we initiated an investigation into the pleiotrophin gene by first cloning its promoter. Transient transfection assays indicated that HOXA5 can specifically activate the pleiotrophin promoter. Promoter deletion, chromatin immunoprecipitation assay, and gel-shift assays were performed to show that HOXA5 can directly bind to one binding site on the pleiotrophin promoter. These data strongly suggest that microarray analysis can successfully identify many potential direct downstream genes of HOXA5. Further functional analysis of these targets will allow us to better understand the diverse functions of HOXA5 in embryonic development and tumorigenesis.
PMID	15757903
GEOID	GSE2241
AUTHORS	Chen H,Rubin E,Zhang H,Chung S,Jie CC,Garrett E,Biswal S,Sukumar S
CONTRIBUTOR	Arthur Liberzon
CONTRIBUTOR_ORG	MSigDB Team
EXACT_SOURCE	Table 4S
FILTERED_BY_SIMILARITY	
EXTERNAL_NAMES_FOR_SIMILAR_TERMS	
EXTERNAL_DETAILS_URL	
SOURCE_MEMBERS	200797_s_at,200798_x_at,201328_at,201329_s_at,201394_s_at,201395_at,201464_x_at,201465_s_at,201466_s_at,201473_at,201502_s_at,201602_s_at,201603_at,201694_s_at,201773_at,201845_s_at,201878_at,202076_at,202081_at,202146_at,202149_at,202220_at,202251_at,202301_s_at,202302_s_at,202393_s_at,202402_s_at,202569_s_at,202643_s_at,202644_s_at,202653_s_at,202672_s_at,202763_at,202768_at,202859_x_at,203155_at,203291_at,203301_s_at,203438_at,203455_s_at,203574_at,203743_s_at,203889_at,203927_at,204094_s_at,204131_s_at,204194_at,204203_at,204234_s_at,204243_at,204285_s_at,204286_s_at,204299_at,204363_at,204435_at,204472_at,204496_at,204499_at,204645_at,204739_at,204748_at,204937_s_at,205047_s_at,205070_at,205122_at,205123_s_at,205193_at,205205_at,205218_at,205263_at,205266_at,205278_at,205579_at,205580_s_at,205583_s_at,205607_s_at,205664_at,205739_x_at,205803_s_at,205807_s_at,205931_s_at,205964_at,206085_s_at,206175_x_at,206235_at,206240_s_at,206332_s_at,206374_at,206613_s_at,206683_at,207147_at,207304_at,207510_at,207536_s_at,207574_s_at,207678_s_at,207941_s_at,208370_s_at,208408_at,208436_s_at,208708_x_at,208720_s_at,208892_s_at,208961_s_at,208966_x_at,208995_s_at,209061_at,209180_at,209181_s_at,209189_at,209211_at,209295_at,209304_x_at,209305_s_at,209451_at,209457_at,209523_at,209545_s_at,209674_at,209824_s_at,209967_s_at,210346_s_at,210512_s_at,210538_s_at,210592_s_at,211038_s_at,211297_s_at,211458_s_at,211506_s_at,212232_at,212474_at,212501_at,212557_at,212569_at,212615_at,212665_at,212684_at,212689_s_at,212721_at,212749_s_at,212781_at,212783_at,212893_at,212900_at,212902_at,212971_at,212997_s_at,213000_at,213097_s_at,213137_s_at,213139_at,213239_at,213281_at,213331_s_at,213341_at,213387_at,213396_s_at,213410_at,213452_at,213483_at,213704_at,213743_at,213872_at,213916_at,213934_s_at,213988_s_at,214016_s_at,214056_at,214061_at,214415_at,214438_at,214683_s_at,214741_at,214751_at,215009_s_at,215043_s_at,215111_s_at,215359_x_at,215493_x_at,216060_s_at,217127_at,217403_s_at,217742_s_at,217779_s_at,217792_at,217828_at,217836_s_at,217873_at,217893_s_at,218023_s_at,218040_at,218192_at,218242_s_at,218303_x_at,218319_at,218371_s_at,218378_s_at,218478_s_at,218518_at,218520_at,218649_x_at,218683_at,218721_s_at,218761_at,218819_at,218880_at,218889_at,218929_at,218940_at,219078_at,219128_at,219228_at,219248_at,219270_at,219286_s_at,219311_at,219394_at,219446_at,219477_s_at,219495_s_at,219540_at,219608_s_at,219676_at,219682_s_at,219793_at,219802_at,219848_s_at,219854_at,219947_at,220046_s_at,220121_at,220205_at,220235_s_at,220346_at,220408_x_at,220444_at,220606_s_at,220746_s_at,220933_s_at,220939_s_at,220941_s_at,221193_s_at,221201_s_at,221213_s_at,221230_s_at,221257_x_at,221258_s_at,221580_s_at,221763_at,221774_x_at,221803_s_at,221842_s_at,221918_at,222028_at,222128_at,222163_s_at,222182_s_at,222233_s_at,222237_s_at,222239_s_at,36711_at,52285_f_at
GENE_SYMBOLS	MCL1,MCL1,ETS2,ETS2,RBM5,RBM5,JUN,JUN,JUN,JUNB,NFKBIA,PPP1R12A,PPP1R12A,EGR1,ADNP,RYBP,ARIH1,BIRC2,IER2,IFRD1,NEDD9,KHDC4,PRPF3,RSRC2,RSRC2,KLF10,CARS1,MARK3,TNFAIP3,TNFAIP3,MARCHF7,ATF3,CASP3,FOSB,CXCL8,SETDB1,CNOT4,DMTF1,STC2,SAT1,NFIL3,TDG,SCG5,NFKBIE,TSC22D2,FOXO3,BACH1,CEBPG,ZNF195,RLF,PMAIP1,PMAIP1,SRSF10,F3,NUP58,GEM,STRN3,,CCNT2,CENPC,PTGS2,ZNF274,ASNS,ING3,TMEFF1,TMEFF1,MAFF,RELB,POLR3F,BCL10,LIF,GAD1,HRH1,HRH1,ALG13,SCYL3,KIN,ZNF107,TRPC1,TUFT1,CREB5,ZNF426,CTH,ZNF222,LIG4,ZNF136,IFI16,DUSP8,TAF1A,ZNF165,DLX2,ZNF45,BDKRB1,TNFRSF9,GADD45B,SOX30,RBM39,RCAN1,,IRF7,EIF5,RBM39,DUSP6,KLF6,IFI16,PPIG,NCOA3,RABGGTB,RABGGTB,FOS,KLF5,TNFRSF10B,GADD45B,GADD45B,TANK,DUSP5,TAF2,RIPK2,CRY1,BMAL1,CREM,CLK4,VEGFA,BIRC3,SAT1,CROCCP2,CDK7,GABARAPL1,CXCL8,FNBP4,AVL9,CEBPB,ZNF451,SMCHD1,CHD9,TIPARP,ZNF3,KDM3A,SREK1,RCHY1,RBBP6,RBBP6,ZZZ3,SEC24A,SEC24A,CARS1,TLK2,MORC3,DNAJC2,PTPN2,SNAI2,PIBF1,,NEK1,FEM1C,ATAD2B,AKAP10,EDRF1,ZNF184,PPWD1,RABGGTB,CCNT2,,ZNF20,ZNF23,SAT1,SFPQ,,TBC1D31,PLG,HLX,CLK1,ZNF131,ZNF468,THAP9-AS1,GUSBP3,TSC22D1,ZNF44,BTN2A1,DAAM1,CTH,ZNF227,WAC,PNRC2,SNX5,SLTM,YY1AP1,CAB39,AKIRIN1,FAM53C,PRPF38B,IP6K2,KMT5B,KRCC1,PELI1,PSPC1,PRKRIP1,ZCCHC8,FAM13B,TBK1,NEMF,PTBP2,ODR4,RNF111,INTS6,FOSL2,NOC3L,CDKN2AIP,VCPKMT,GPATCH2,C2orf42,ZNF331,THUMPD2,CHAC1,RBM15,CEP76,PGS1,RIC8B,THSD1,ZNF180,ZNF267,FBXO38,ZSCAN16,TBX3,SNX16,PYROXD1,ZNF432,ZNF14,CLEC4A,CCNL1,LINS1,TPTE,LRIF1,MTHFD2L,SUPT20H,ZNF557,ADPRM,UIMC1,TUT7,DPP8,C21orf91,ZCCHC10,ZNF155,ZNF280D,ARID4B,FBXO38,KIF18A,TAF1D,JMJD1C,SUPT20H,NRBF2,ZNF131,CDK17,ZNF45,CACNB2-AS1,AFG2B,CNOT2,DCLRE1C,ZNF112,INTS6,MAFF,CEP76
FOUNDER_NAMES