STANDARD_NAME CHEN_LVAD_SUPPORT_OF_FAILING_HEART_UP SYSTEMATIC_NAME M12300 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/CHEN_LVAD_SUPPORT_OF_FAILING_HEART_UP NAMESPACE AFFY_HG_U133 DESCRIPTION_BRIEF Up-regulated genesin the left ventricle myocardium of patients with heart failure following implantation of LVAD (left ventricular assist device). DESCRIPTION_FULL Chronic unloading of the failing heart with a left ventricular assist device (LVAD) can decrease cardiac mass and myocyte size and has the potential to improve contractile function. To study the effect of chronic ventricular unloading on myocardial gene expression, a microarray (U133A, Affymetrix) profiling gene expression was compared before and after LVAD support in seven patients with idiopathic dilated cardiomyopathy and end-stage heart failure. On average, 1,374 +/- 155 genes were reported as increased and 1,629 +/- 45 as decreased after LVAD support. A total of 130 gene transcripts achieved the strict criteria for upregulation and 49 gene transcripts for downregulation after LVAD support. Upregulated genes included a large proportion of transcription factors, genes related to cell growth/apoptosis/DNA repair, cell structure proteins, metabolism, and cell signaling/communication. LVAD support resulted in downregulation of genes for a group of cytokines. To validate the array data, 10 altered genes were confirmed by real-time RT-PCR. Further study showed that the phosphoinositide-3-kinase-forkhead protein pathway and proteins related to nitric oxide synthesis, including eNOS and dimethylarginine dimethylaminohydrolase isoform 1 (DDAH1, an enzyme regulating endogenous nitric oxide synthase activity), were significantly increased during the cardiac remodeling process. Increased eNOS and DDAH1 expression after LVAD support may contribute to improved endothelial function of the failing hearts. PMID 12824457 GEOID GSE430 AUTHORS Chen Y,Park S,Li Y,Missov E,Hou M,Han X,Hall JL,Miller LW,Bache RJ CONTRIBUTOR John Newman CONTRIBUTOR_ORG University of Washington EXACT_SOURCE Table 3S: Fold Change = arrow up FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS 200648_s_at,200797_s_at,200931_s_at,200940_s_at,201009_s_at,201147_s_at,201148_s_at,201150_s_at,201196_s_at,201235_s_at,201236_s_at,201289_at,201464_x_at,201466_s_at,201502_s_at,201505_at,201531_at,201539_s_at,201616_s_at,201761_at,202007_at,202284_s_at,202628_s_at,202672_s_at,202861_at,202887_s_at,203140_at,203542_s_at,203543_s_at,203574_at,203592_s_at,203645_s_at,203872_at,203951_at,203973_s_at,204121_at,204131_s_at,204132_s_at,204326_x_at,204560_at,204679_at,204979_s_at,205239_at,205357_s_at,205383_s_at,205610_at,205766_at,205883_at,205960_at,206029_at,206243_at,206461_x_at,206698_at,206855_s_at,207513_s_at,207574_s_at,207761_s_at,207876_s_at,208581_x_at,208763_s_at,208791_at,208792_s_at,209094_at,209101_at,209122_at,209147_s_at,209183_s_at,209184_s_at,209185_s_at,209304_x_at,209305_s_at,209834_at,209981_at,210096_at,210275_s_at,210298_x_at,210299_s_at,210655_s_at,210764_s_at,210809_s_at,211456_x_at,211651_s_at,212185_x_at,212239_at,212240_s_at,212249_at,212418_at,212501_at,212538_at,212589_at,212590_at,212665_at,212713_at,212724_at,212742_at,212817_at,212848_s_at,212859_x_at,213139_at,213489_at,213792_s_at,214505_s_at,214721_x_at,214724_at,215001_s_at,215049_x_at,215870_s_at,217165_x_at,217202_s_at,217546_at,217741_s_at,217988_at,218041_x_at,218062_x_at,218428_s_at,218611_at,218618_s_at,218678_at,218697_at,218723_s_at,218880_at,219315_s_at,219343_at,219371_s_at,219728_at,219804_at,219911_s_at,220924_s_at,221232_s_at,36829_at GENE_SYMBOLS GLUL,MCL1,VCL,RERE,TXNIP,TIMP3,TIMP3,TIMP3,AMD1,BTG2,BTG2,CCN1,JUN,JUN,NFKBIA,LAMB1,ZFP36,FHL1,CALD1,MTHFD2,NID1,CDKN1A,SERPINE1,ATF3,PER1,DDIT4,BCL6,KLF9,KLF9,NFIL3,FSTL3,CD163,ACTA1,CNN1,CEBPD,GADD45G,FOXO3,FOXO3,MT1X,FKBP5,KCNK1,SH3BGR,AREG,AGTR1,ZBTB20,MYOM1,TCAP,ZBTB16,PDK4,ANKRD1,TIMP4,MT1H,XK,HYAL2,ZNF189,GADD45B,METTL7A,FLNC,MT1X,TSC22D3,CLU,CLU,DDAH1,CCN2,PLIN2,PLPP1,DEPP1,IRS2,IRS2,GADD45B,GADD45B,CHST3,CSDC2,CYP4B1,ZFAND5,FHL1,FHL1,FOXO3,CCN1,POSTN,MT1H,LAMB1,MT2A,PIK3R1,PIK3R1,PIK3R1,ELF1,CEBPB,DOCK9,RRAS2,RRAS2,TIPARP,MFAP4,RND3,RNF115,DNAJB5,AOPEP,MT1E,SNAI2,MAPRE2,INSR,FHL1,CDC42EP4,DIXDC1,GLUL,CD163,PLA2G5,MT1F,GLUL,MT1M,ZFAND5,CCNB1IP1,SLC38A2,CDC42EP4,REV1,IER5,FNDC3B,NES,NCKIPSD,RGCC,FOSL2,TMEM204,CDC37L1,KLF2,MYOT,SYNPO2L,SLCO4A1,SLC38A2,ANKRD2,PER1 FOUNDER_NAMES