STANDARD_NAME DHIMAN_PBMC_ATTENUVAX_AGE_15_25YO_SUBQ_7_OR_14DY_DN SYSTEMATIC_NAME M40891 COLLECTION C7:VAX MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/DHIMAN_PBMC_ATTENUVAX_AGE_15_25YO_SUBQ_7_OR_14DY_DN NAMESPACE HUMAN_SEQ_ACCESSION DESCRIPTION_BRIEF Genes down-regulated in peripheral blood mononuclear cell 7d or 14d vs 0d in adults (15-25) after exposure to Attenuvax , time point 7 or 14D , administered subcutaneous DESCRIPTION_FULL Cellular immunity to measles vaccination is not fully understood at the effector response and gene expression levels. We enrolled 15 healthy individuals (15-25 years old) previously vaccinated with two doses of measles-mumps-rubella-II vaccine to characterize their cellular immunity. We detected a spectrum of lymphoproliferative response (median stimulation indices of 3.4), low precursor frequencies of interferon-gamma (median 0.11%) and interleukin-4 (median 0.05%) by Elispot, and cosecretion of Th1 and Th2 cytokines after measles virus stimulation. Further, global gene expression was examined in five subjects from this cohort after vaccination with an additional dose of measles vaccine (Attenuax, Merck) to identify the genes involved in measles immunity. Linear mixed effect models were used to identify genes significantly up or downregulated in vivo between baseline and Days 7 and 14 after measles vaccination. Measles vaccination induced upregulation of a set of 80 genes, which play a role in measles immunity, signal transduction, apoptosis, cell proliferation, and metabolic pathways. Among the 34 genes that were downregulated, only interferon-alpha is known to have a direct role in measles immunity. This study suggests that measles vaccination leads to activation of multiple cellular mechanisms that can override the immunosuppressant effects of the measles virus and induce immunity. PMID 16571413 GEOID AUTHORS Dhiman N,Ovsyannikova IG,Oberg AL,Grill DE,Jacobson RM,Poland GA CONTRIBUTOR HIPC SIGNATURES CONTRIBUTOR_ORG NIAID/HIPC SIGNATURES EXACT_SOURCE Table 4 FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS AF045941,AF069517,AL080058,CBS,D21089,D86981,D87717,J00210,L35546,L42452,M11433,M23234,M34309,M37190,M73239,OPCML,S43855,U26266,U97669 GENE_SYMBOLS SCEL,RBM6,MOXD1,CBS,XPC,APPBP2,ARHGAP11A,IFNA1,GCLM,PDK3,RBP1,ABCB4,ERBB3,RIN2,HGF,OPCML,RCVRN,DHPS,NOTCH3 FOUNDER_NAMES