STANDARD_NAME	DUNNE_TARGETS_OF_AML1_MTG8_FUSION_DN
SYSTEMATIC_NAME	M6469
COLLECTION	C2:CGP
MSIGDB_URL	https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/DUNNE_TARGETS_OF_AML1_MTG8_FUSION_DN
NAMESPACE	HUMAN_GENE_SYMBOL
DESCRIPTION_BRIEF	Genes down-regulated in Kasumi-1 cells (acute myeloid leukaemia (AML) with the t(8;21) translocation) after knockdown of the AML1 MTG8 fusion [GeneID=861;862] by RNAi.
DESCRIPTION_FULL	The chromosomal translocation t(8;21) is associated with 10-15% of all cases of acute myeloid leukaemia (AML). The resultant fusion protein AML1/MTG8 interferes with haematopoietic gene expression and is an important regulator of leukaemogenesis. We studied the effects of small interfering RNA (siRNA)-mediated AML1/MTG8 depletion on global gene expression in t(8;21)-positive leukaemic cell lines and in primary AML blasts using cDNA arrays, oligonucleotide arrays and real-time reverse transcription-polymerase chain reaction (RT-PCR). Suppression of AML1/MTG8 results in the increased expression of genes associated with myeloid differentiation, such as AZU1, BPI, CTSG, LYZ and RNASE2 as well as of antiproliferative genes such as IGFBP7, MS4A3 and SLA both in blasts and in cell lines. Furthermore, expression levels of several genes affiliated with drug resistance or indicative of poor prognosis AML (BAALC, CD34, PRG2, TSPAN7) are affected by AML1/MTG8 depletion. In conclusion, siRNA-mediated suppression of AML1/MTG8 cause very similar changes in gene expression pattern in t(8;21)-positive cell lines and in primary AML blasts. Furthermore, the results suggest that the specific targeting of AML1/MTG8 function may be a promising approach for complementing existing treatment strategies.
PMID	16652140
GEOID	GSE2049
AUTHORS	Dunne J,Cullmann C,Ritter M,Soria NM,Drescher B,Debernardi S,Skoulakis S,Hartmann O,Krause M,Krauter J,Neubauer A,Young BD,Heidenreich O
CONTRIBUTOR	Leona Saunders
CONTRIBUTOR_ORG	MSigDB Team
EXACT_SOURCE	Table 1-2: siAGF1/siAGF6 < 1
FILTERED_BY_SIMILARITY	
EXTERNAL_NAMES_FOR_SIMILAR_TERMS	
EXTERNAL_DETAILS_URL	
SOURCE_MEMBERS	ADAM12,BAALC,CD34,CKB,DEPDC6,DUSP6,ECM1,EDIL3,ELK3,FOSL2,ID1,IFI16,MEF2C,MTSS1,RANBP2,RUNX1T1,SAMSN1,SLC2A3,TSPAN7
GENE_SYMBOLS	ADAM12,BAALC,CD34,CKB,DEPTOR,DUSP6,ECM1,EDIL3,ELK3,FOSL2,ID1,IFI16,MEF2C,MTSS1,RANBP2,RUNX1T1,SAMSN1,SLC2A3,TSPAN7
FOUNDER_NAMES