STANDARD_NAME EPPERT_HSC_R SYSTEMATIC_NAME M19231 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/EPPERT_HSC_R NAMESPACE AFFY_HG_U133 DESCRIPTION_BRIEF Genes up-regulated in human hematopoietic stem cell (HSC) enriched populations compared to committed progenitors and mature cells. DESCRIPTION_FULL Xenograft studies indicate that some solid tumors and leukemias are organized as cellular hierarchies sustained by cancer stem cells (CSCs). Despite the promise of the CSC model, its relevance in humans remains uncertain. Here we show that acute myeloid leukemia (AML) follows a CSC model on the basis of sorting multiple populations from each of 16 primary human AML samples and identifying which contain leukemia stem cells (LSCs) using a sensitive xenograft assay. Analysis of gene expression from all functionally validated populations yielded an LSC-specific signature. Similarly, a hematopoietic stem cell (HSC) gene signature was established. Bioinformatic analysis identified a core transcriptional program shared by LSCs and HSCs, revealing the molecular machinery underlying stemness properties. Both stem cell programs were highly significant independent predictors of patient survival and were found in existing prognostic signatures. Thus, determinants of stemness influence the clinical outcome of AML, establishing that LSCs are clinically relevant and not artifacts of xenotransplantation. PMID 21873988 GEOID GSE30377 AUTHORS Eppert K,Takenaka K,Lechman ER,Waldron L,Nilsson B,van Galen P,Metzeler KH,Poeppl A,Ling V,Beyene J,Canty AJ,Danska JS,Bohlander SK,Buske C,Minden MD,Golub TR,Jurisica I,Ebert BL,Dick JE CONTRIBUTOR Kolja Eppert CONTRIBUTOR_ORG McGill University EXACT_SOURCE Supplemental Table 8 FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS 200033_at,200672_x_at,200962_at,201466_s_at,201625_s_at,201695_s_at,201889_at,201917_s_at,201952_at,202551_s_at,202724_s_at,202822_at,202842_s_at,202932_at,203139_at,203372_s_at,203394_s_at,203875_at,204069_at,204304_s_at,204753_s_at,204754_at,204755_x_at,204917_s_at,205376_at,205453_at,205501_at,205984_at,206099_at,206310_at,206385_s_at,206478_at,206674_at,206683_at,207563_s_at,207564_x_at,208523_x_at,208527_x_at,208707_at,208820_at,208891_at,208892_s_at,208988_at,209020_at,209146_at,209487_at,209560_s_at,209676_at,209728_at,209907_s_at,209911_x_at,209993_at,209994_s_at,210664_s_at,210665_at,210942_s_at,211597_s_at,212071_s_at,212176_at,212179_at,212314_at,212488_at,212750_at,212764_at,212958_x_at,213056_at,213094_at,213355_at,213510_x_at,213541_s_at,213714_at,213750_at,213844_at,214327_x_at,214349_at,215388_s_at,215779_s_at,217975_at,218280_x_at,218332_at,218379_at,218627_at,218723_s_at,218764_at,218772_x_at,218899_s_at,218901_at,218966_at,218971_s_at,219054_at,219497_s_at,219559_at,219648_at,220122_at,220416_at,221458_at,221773_at,221833_at,221841_s_at,221942_s_at,222067_x_at,222164_at,222315_at,222735_at,222815_at,225629_s_at,226206_at,226420_at,226545_at,226547_at,226985_at,228465_at,228570_at,228857_at,228904_at,228915_at,229287_at,229344_x_at,230389_at,230698_at,230788_at,232098_at,232231_at,234994_at,235048_at,235199_at,235252_at,235490_at,235826_at,236193_at,238041_at,238488_at,238633_at,238974_at,239328_at,239451_at,239835_at,240165_at,241756_at,243010_at,243092_at,243835_at,244110_at,244447_at,244519_at,41577_at,60084_at GENE_SYMBOLS DDX5,SPTBN1,RPL31,JUN,INSIG1,PNP,FAM3C,SLC25A36,ALCAM,CRIM1,FOXO1,LPP,DNAJB9,YES1,DAPK1,SOCS2,HES1,SMARCA1,MEIS1,PROM1,HLF,HLF,HLF,MLLT3,INPP4B,HOXB2,PDE10A,CRHBP,PRKCH,SPINK2,ANK3,FAM30A,FLT3,ZNF165,OGT,OGT,H2BC10,H2BC6,EIF5,PTK2,DUSP6,DUSP6,KDM2A,OSER1,MSMO1,RBPMS,DLK1,TFPI,HLA-DRB4,ITSN2,H2BC5,ABCB1,ABCB1,TFPI,TFPI,ST3GAL6,HOPX,SPTBN1,PNISR,PNISR,SEL1L3,COL5A1,PPP1R16B,ZEB1,PAM,FRMD4B,ADGRG6,ST3GAL6,FAM106A,ERG,CACNB2,RSL1D1,HOXA5,TPT1,ENSG00000275616,CFH,H2BC8,TCEAL9,H2AC18,BEX1,RBM7,DRAM1,RGCC,PRKCH,TMEM38B,BAALC,PLSCR4,MYO5C,WDR91,NPR3,BCL11A,SLC17A9,MREG,MCTP1,ATP8B4,HTR1F,ELK3,LONP2,KLF4,GUCY1A1,H2BC5,RPS20P22,,TMEM38B,RLIM,ZBTB4,MAFK,MECOM,CD109,KAT6A,FGD5,,ABTB3,GNL1,HOXB3,DACH1,PCNX1,RIMKLB,FNBP1,CALN1,GCNT2,DST,RUNX2,TMEM200A,FAM169A,RNF125,KSR1,TMEM107,,,TCF12,IPO11,EPC1,C2orf69,RCSD1,HSP90B1,KBTBD8,,SMARCA2,MSI2,PAN3-AS1,ZDHHC21,KMT2A,,ASXL1,PPP1R16B,CYLD FOUNDER_NAMES