STANDARD_NAME FOURNIER_ACINAR_DEVELOPMENT_EARLY_DN SYSTEMATIC_NAME M14136 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/FOURNIER_ACINAR_DEVELOPMENT_EARLY_DN NAMESPACE AFFY_HG_U133 DESCRIPTION_BRIEF Genes down-regulated early in HMEC cells (mammary epithelium) during acinar development in vitro. DESCRIPTION_FULL Nonmalignant human mammary epithelial cells (HMEC) seeded in laminin-rich extracellular matrix (lrECM) form polarized acini and, in doing so, transit from a disorganized proliferating state to an organized growth-arrested state. We hypothesized that the gene expression pattern of organized and growth-arrested HMECs would share similarities with breast tumors with good prognoses. Using Affymetrix HG-U133A microarrays, we analyzed the expression of 22,283 gene transcripts in 184 (finite life span) and HMT3522 S1 (immortal nonmalignant) HMECs on successive days after seeding in a lrECM assay. Both HMECs underwent growth arrest in G0-G1 and differentiated into polarized acini between days 5 and 7. We identified gene expression changes with the same temporal pattern in both lines and examined the expression of these genes in a previously published panel of microarray data for 295 breast cancer samples. We show that genes that are significantly lower in the organized, growth-arrested HMEC than in their proliferating counterparts can be used to classify breast cancer patients into poor and good prognosis groups with high accuracy. This study represents a novel unsupervised approach to identifying breast cancer markers that may be of use clinically. PMID 16849555 GEOID GSE8096 AUTHORS Fournier MV,Martin KJ,Kenny PA,Xhaja K,Bosch I,Yaswen P,Bissell MJ CONTRIBUTOR Arthur Liberzon CONTRIBUTOR_ORG MSigDB Team EXACT_SOURCE Table 1S: de FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS 201109_s_at,201920_at,208433_s_at,212197_x_at,217997_at,222108_at GENE_SYMBOLS THBS1,SLC20A1,LRP8,MPRIP,NAP1L1,AMIGO2 FOUNDER_NAMES