STANDARD_NAME	FOURNIER_ACINAR_DEVELOPMENT_LATE_DN
SYSTEMATIC_NAME	M13202
COLLECTION	C2:CGP
MSIGDB_URL	https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/FOURNIER_ACINAR_DEVELOPMENT_LATE_DN
NAMESPACE	AFFY_HG_U133
DESCRIPTION_BRIEF	Genes down-regulated late in HMEC cells (mammary epithelium) during acinar development in vitro.
DESCRIPTION_FULL	Nonmalignant human mammary epithelial cells (HMEC) seeded in laminin-rich extracellular matrix (lrECM) form polarized acini and, in doing so, transit from a disorganized proliferating state to an organized growth-arrested state. We hypothesized that the gene expression pattern of organized and growth-arrested HMECs would share similarities with breast tumors with good prognoses. Using Affymetrix HG-U133A microarrays, we analyzed the expression of 22,283 gene transcripts in 184 (finite life span) and HMT3522 S1 (immortal nonmalignant) HMECs on successive days after seeding in a lrECM assay. Both HMECs underwent growth arrest in G0-G1 and differentiated into polarized acini between days 5 and 7. We identified gene expression changes with the same temporal pattern in both lines and examined the expression of these genes in a previously published panel of microarray data for 295 breast cancer samples. We show that genes that are significantly lower in the organized, growth-arrested HMEC than in their proliferating counterparts can be used to classify breast cancer patients into poor and good prognosis groups with high accuracy. This study represents a novel unsupervised approach to identifying breast cancer markers that may be of use clinically.
PMID	16849555
GEOID	GSE8096
AUTHORS	Fournier MV,Martin KJ,Kenny PA,Xhaja K,Bosch I,Yaswen P,Bissell MJ
CONTRIBUTOR	Arthur Liberzon
CONTRIBUTOR_ORG	MSigDB Team
EXACT_SOURCE	Table 1S: dl
FILTERED_BY_SIMILARITY	
EXTERNAL_NAMES_FOR_SIMILAR_TERMS	
EXTERNAL_DETAILS_URL	
SOURCE_MEMBERS	200790_at,200801_x_at,201714_at,202580_x_at,203499_at,203856_at,204014_at,204033_at,204092_s_at,204170_s_at,205014_at,206467_x_at,208637_x_at,209714_s_at,209773_s_at,212190_at,214805_at,218349_s_at,218456_at,218542_at,218663_at,219918_s_at
GENE_SYMBOLS	ODC1,ACTB,TUBG1,FOXM1,EPHA2,VRK1,DUSP4,TRIP13,AURKA,CKS2,FGFBP1,TNFRSF6B,ACTN1,CDKN3,RRM2,SERPINE2,EIF4A1,ZWILCH,CAPRIN2,CEP55,NCAPG,ASPM
FOUNDER_NAMES