STANDARD_NAME GENTILE_UV_RESPONSE_CLUSTER_D4 SYSTEMATIC_NAME M15468 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/GENTILE_UV_RESPONSE_CLUSTER_D4 NAMESPACE HUMAN_SEQ_ACCESSION DESCRIPTION_BRIEF Cluster d4: genes progressively down-regulated in WS1 cells (fibroblast) through 12 h after irradiation with high dose UV-C. DESCRIPTION_FULL DNA damage caused by UV radiation initiates cellular recovery mechanisms, which involve activation of DNA damage response pathways, cell cycle arrest and apoptosis. To assess cellular transcriptional responses to UVC-induced DNA damage we compared time course responses of human skin fibroblasts to low and high doses of UVC radiation known to induce a transient cellular replicative arrest or apoptosis, respectively. UVC radiation elicited >3-fold changes in 460 out of 12,000 transcripts and 89% of these represented downregulated transcripts. Only 5% of the regulated genes were common to both low and high doses of radiation. Cells inflicted with a low dose of UVC exhibited transcription profiles demonstrating transient regulation followed by recovery, whereas the responses were persistent after the high dose. A detailed clustering analysis and functional classification of the targets implied regulation of biologically divergent responses and suggested involvement of transcriptional and translational machinery, inflammatory, anti-proliferative and anti-angiogenic responses. The data support the notion that UVC radiation induces prominent, dose-dependent downregulation of transcription. However, the data strongly suggest that transcriptional repression is also target gene selective. Furthermore, the results demonstrate that dose-dependent induction of cell cycle arrest and apoptosis by UVC radiation are transcriptionally highly distinct responses. PMID 12907719 GEOID GSE713 AUTHORS Gentile M,Latonen L,Laiho M CONTRIBUTOR John Newman CONTRIBUTOR_ORG University of Washington EXACT_SOURCE Table 2S: Cluster d4 FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS AA442799,AA630312,AB004066,AB007930,AB011004,AB014458,AB017365,AB018319,AB023207,AB028976,AF000982,AF007142,AF011468,AF022385,AF038009,AF050110,AF054186,AF070570,AF090988,AI655015,AJ131245,AJ223321,AJ238764,AL049471,AL049933,AL050268,AL080113,AL096858,D38551,D43951,D78514,D79993,D79994,D80012,L20852,M23115,M25753,M57763,M68891,M92843,U23946,U28386,U37352,U53445,U76247,U89278,U90268,W27541,X62534,X78947,X80692,X92518,Y08614,Z22535,Z24724 GENE_SYMBOLS THUMPD1,SMURF2,BHLHE40,POGZ,UAP1,USP1,FZD7,UFL1,CHSY1,SAMD4A,DDX3X,DICER1,AURKA,PDCD10,TPST1,KLF10,EEF1E1,RAPGEF2,SNRNP40,DUSP7,SEC24B,ZBTB18,GNE,ARID5B,GNAI1,RAB1A,DDX17,SPEN,RAD21,PUM1,UBE2G1,CLINT1,KANK1,USP10,SLC20A2,ATP2A2,CCNB1,ARF6,GATA2,ZFP36,RBM5,KPNA2,PPP2R5C,FILIP1L,SIAH1,PHC2,KRIT1,,HMGB2,CCN2,MAPK6,HMGA2,XPO1,BMPR1A,ATP13A3 FOUNDER_NAMES