STANDARD_NAME GINESTIER_BREAST_CANCER_20Q13_AMPLIFICATION_DN SYSTEMATIC_NAME M19988 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/GINESTIER_BREAST_CANCER_20Q13_AMPLIFICATION_DN NAMESPACE AFFY_HG_U133 DESCRIPTION_BRIEF Genes down-regulated in metastatic breast cancer tumors having type 2 amplification in the 20q13 region; involves MYBL2, STK6 and ZNF217 [GeneID=4605;6790;7764] DESCRIPTION_FULL PURPOSE: Amplification of chromosomal region 20q13 occurs in breast cancer but remains poorly characterized. EXPERIMENTAL DESIGN: To establish the frequency of 20q13 amplification and select the amplified cases to be studied, we used fluorescence in situ hybridization of bacterial artificial chromosome probes for three 20q13 loci (MYBL2, STK6, ZNF217) on sections of tissue microarrays containing 466 primary carcinoma samples. We used Affymetryx whole-genome DNA microarrays to establish the gene expression profiles of 20q13-amplified tumors and quantitative reverse transcription-PCR to validate the results. RESULTS: We found 36 (8%) 20q13-amplified samples. They were distributed in two types: type 1 tumors showed ZNF217 amplification only, whereas type 2 tumors showed amplification at two or three loci. Examination of the histoclinical features of the amplified tumors showed two strikingly opposite data. First, type 1 tumors were more frequently lymph node-negative tumors but were paradoxically associated with a poor prognosis. Second, type 2 tumors were more frequently lymph node-positive tumors but were paradoxically associated with a good prognosis. Type 1 and type 2 showed different gene expression profiles. No 20q13 gene could be associated with type 1 amplification, whereas several 20q13 genes were overexpressed in type 2 tumors. CONCLUSIONS: Our results suggest that amplified tumors of types 1 and 2 are two distinct entities resulting from two different mechanisms and associated to different prognosis. PMID 16899599 GEOID AUTHORS Ginestier C,Cervera N,Finetti P,Esteyries S,Esterni B,Adélaïde J,Xerri L,Viens P,Jacquemier J,Charafe-Jauffret E,Chaffanet M,Birnbaum D,Bertucci F CONTRIBUTOR Leona Saunders CONTRIBUTOR_ORG MSigDB Team EXACT_SOURCE Table 5S FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS 1555411_a_at,1555960_at,1556090_at,1556597_a_at,1560089_at,200041_s_at,200754_x_at,201166_s_at,201188_s_at,201189_s_at,201545_s_at,201555_at,201578_at,201585_s_at,201664_at,201837_s_at,201945_at,202005_at,202140_s_at,202153_s_at,202155_s_at,202220_at,202253_s_at,202326_at,202385_s_at,202474_s_at,202510_s_at,202560_s_at,202633_at,202642_s_at,202880_s_at,203091_at,203094_at,203183_s_at,203205_at,203254_s_at,203379_at,203520_s_at,203521_s_at,203569_s_at,203694_s_at,203802_x_at,203881_s_at,203944_x_at,203977_at,204158_s_at,204166_at,204275_at,204522_at,204658_at,204978_at,205393_s_at,205642_at,205658_s_at,205839_s_at,207127_s_at,207196_s_at,207394_at,207556_s_at,207939_x_at,208044_s_at,208132_x_at,208149_x_at,208159_x_at,208313_s_at,208625_s_at,208686_s_at,208829_at,209214_s_at,209236_at,209430_at,209680_s_at,209689_at,209907_s_at,210208_x_at,210252_s_at,210410_s_at,211012_s_at,211256_x_at,211270_x_at,211282_x_at,211982_x_at,211996_s_at,212022_s_at,212036_s_at,212081_x_at,212193_s_at,212201_at,212232_at,212235_at,212733_at,212757_s_at,212856_at,213313_at,213318_s_at,213359_at,213378_s_at,213460_x_at,213534_s_at,213596_at,213670_x_at,213690_s_at,213773_x_at,213842_x_at,214048_at,214100_x_at,214201_x_at,214438_at,214780_s_at,214882_s_at,215184_at,215364_s_at,215493_x_at,215780_s_at,215985_at,216036_x_at,216237_s_at,216905_s_at,216945_x_at,217297_s_at,218076_s_at,218157_x_at,218371_s_at,218397_at,218420_s_at,218443_s_at,218587_s_at,218600_at,218733_at,218781_at,219058_x_at,219178_at,219284_at,219378_at,219394_at,219423_x_at,219470_x_at,219639_x_at,219974_x_at,219988_s_at,220046_s_at,220349_s_at,220589_s_at,220617_s_at,220896_at,221050_s_at,221525_at,221755_at,221774_x_at,221816_s_at,221819_at,221857_s_at,221878_at,221879_at,222280_at,222592_s_at,222612_at,223056_s_at,223088_x_at,223162_s_at,223365_at,223450_s_at,223921_s_at,224326_s_at,224521_s_at,224627_at,224673_at,224884_at,225057_at,225077_at,225157_at,225451_at,225586_at,225738_at,225819_at,226301_at,226389_s_at,226823_at,226877_at,227025_at,227146_at,227170_at,227463_at,227502_at,227591_at,227668_at,227793_at,227930_at,228023_x_at,228192_at,228277_at,228387_at,228515_at,228828_at,228847_at,228902_at,229086_at,229120_s_at,229433_at,229445_at,229548_at,229631_at,229795_at,229872_s_at,229914_at,229926_at,230526_at,231777_at,232667_at,232683_s_at,235217_at,235441_at,235535_x_at,236247_at,236309_x_at,236314_at,239422_at,239973_at,240286_at,241354_at,241825_at,241991_at,242611_at,243259_at,244241_x_at,244271_at,37586_at,38398_at,47608_at,64408_s_at GENE_SYMBOLS CCNL1,HINT1,,,SLC25A25-AS1,DDX39B,SRSF2,PUM1,ITPR3,ITPR3,PABPN1,MCM3,PODXL,SFPQ,SMC4,SUPT7L,FURIN,ST14,CLK3,NUP62,NUP214,KHDC4,DNM2,EHMT2,TCOF1,HCFC1,TNFAIP2,CHTOP,TOPBP1,TRRAP,CYTH1,FUBP1,MAD2L1BP,SMARCD1,KDM4A,TLN1,RPS6KA1,ZNF318,ZNF318,OFD1,DHX16,NSUN5,DMD,BTN2A1,TAFAZZIN,TCIRG1,SBNO2,CAPN15,DXO,TRA2A,CLASRP,CHEK1,CNTRL,SNAPC4,TSPOAP1,HNRNPH3,TNIP1,,DGKZ,RNPS1,PPARD,PRRC2A,DDX11,DDX11,SF1,EIF4G1,BRD2,TAPBP,EWSR1,SLC23A2,BTAF1,KIFC1,CCDC93,ITSN2,BAG6,MADD,MSH5,PML,BTN2A1,PTBP1,TNFRSF25,XPO6,NPIPB3,MKI67,PNN,PRRC2A,LARP1,ANKLE2,FNBP4,PLXND1,RUBCN,CAMK2G,GRAMD4,RABGAP1,BAG6,HNRNPD,DDX11,NSUN5P1,PASK,CASP4,NSUN5P1,PTOV1-AS2,NSUN5,NSUN5,MBD4,NSUN5,PRRC2A,HLX,MYO9B,SRSF2,DAPK2,SZT2,BTN2A1,SET,POLR1HASP,WDTC1,MCM5,ST14,PASK,MYO9B,ARHGAP17,CDC42SE1,PSPC1,,PROSER1,DAZAP1,POGLUT1,LIMD2,MSL2,SMC6,TINAGL1,QTRT2,HSPBAP1,NAA16,PGS1,TNFRSF25,CCNJ,PARP6,ECHDC1,RNF220,CCNL1,ENGASE,ITFG2,ZNF532,FBXL18,GTPBP2,ZMIZ2,EHBP1L1,SUPT20H,PHF11,RAB35,TJAP1,C2orf68,CALML4,TMEM147-AS1,ACSL5,PSPC1,XPO5,ECHDC1,DENND11,DHX37,COG3,GBA2,PCGF6,CCDC77,GBA2,LENG8,AKAP13,SLC15A4,CHD2,MLXIP,GRIPAP1,DPH7,RAPGEF1,TBRG1,SLC18B1,RAPGEF1,PHACTR4,,PPHLN1,QSOX2,ZNF316,ACE,DENND11,SH3BP5-AS1,TEPSIN,MIRLET7D,AGO4,AMY2B,UQCC2,FBXL19,LINC01355,POLR1A,TSPOAP1-AS1,EXOC3,NUP214,ZNF436-AS1,CDC42SE1,RBM26,CYBA,,DNHD1,MON2,,,MIR3682,TMC6,CSNK2B,,PARP6,LINC01004,,,NSUN4,ZMIZ2,EPM2AIP1,GPC2,SNHG26,OFD1,ASMTL-AS1,TYW5,,DOCK7,ATXN7,,,ZNF142,MADD,TJAP1,CALML4 FOUNDER_NAMES