STANDARD_NAME GRADE_METASTASIS_DN SYSTEMATIC_NAME M13597 COLLECTION ARCHIVED:C2_CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/GRADE_METASTASIS_DN NAMESPACE UniGene_ID DESCRIPTION_BRIEF Down-regulated genes in colon carcinoma tumors with lymph node metastases. DESCRIPTION_FULL To characterize patterns of global transcriptional deregulation in primary colon carcinomas, we did gene expression profiling of 73 tumors [Unio Internationale Contra Cancrum stage II (n = 33) and stage III (n = 40)] using oligonucleotide microarrays. For 30 of the tumors, expression profiles were compared with those from matched normal mucosa samples. We identified a set of 1,950 genes with highly significant deregulation between tumors and mucosa samples (P < 1e-7). A significant proportion of these genes mapped to chromosome 20 (P = 0.01). Seventeen genes had a >5-fold average expression difference between normal colon mucosa and carcinomas, including up-regulation of MYC and of HMGA1, a putative oncogene. Furthermore, we identified 68 genes that were significantly differentially expressed between lymph node-negative and lymph node-positive tumors (P < 0.001), the functional annotation of which revealed a preponderance of genes that play a role in cellular immune response and surveillance. The microarray-derived gene expression levels of 20 deregulated genes were validated using quantitative real-time reverse transcription-PCR in >40 tumor and normal mucosa samples with good concordance between the techniques. Finally, we established a relationship between specific genomic imbalances, which were mapped for 32 of the analyzed colon tumors by comparative genomic hybridization, and alterations of global transcriptional activity. Previously, we had conducted a similar analysis of primary rectal carcinomas. The systematic comparison of colon and rectal carcinomas revealed a significant overlap of genomic imbalances and transcriptional deregulation, including activation of the Wnt/beta-catenin signaling cascade, suggesting similar pathogenic pathways. PMID 17210682 GEOID AUTHORS Grade M,Hörmann P,Becker S,Hummon AB,Wangsa D,Varma S,Simon R,Liersch T,Becker H,Difilippantonio MJ,Ghadimi BM,Ried T CONTRIBUTOR Jessica Robertson CONTRIBUTOR_ORG MSigDB Team EXACT_SOURCE Table 2C FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS Hs.119192,Hs.119251,Hs.119591,Hs.12341,Hs.131431,Hs.144941,Hs.157351,Hs.172772,Hs.176615,Hs.194385,Hs.197320,Hs.20521,Hs.211594,Hs.239499,Hs.292575,Hs.301961,Hs.367842,Hs.368149,Hs.371282,Hs.414565,Hs.415846,Hs.432121,Hs.432453,Hs.433291,Hs.434937,Hs.443831,Hs.446260,Hs.446427,Hs.467192,Hs.47062,Hs.490394,Hs.5120,Hs.515472,Hs.515876,Hs.518123,Hs.520974,Hs.521056,Hs.524216,Hs.530096,Hs.530479,Hs.624,Hs.75348,Hs.77578,Hs.78466,Hs.89545 GENE_SYMBOLS ,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,,, FOUNDER_NAMES