STANDARD_NAME	GRAHAM_CML_QUIESCENT_VS_NORMAL_DIVIDING_DN
SYSTEMATIC_NAME	M13254
COLLECTION	C2:CGP
MSIGDB_URL	https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/GRAHAM_CML_QUIESCENT_VS_NORMAL_DIVIDING_DN
NAMESPACE	AFFY_HG_U133
DESCRIPTION_BRIEF	Genes down-regulated in quescent CD34+ [GeneID=8842] cells isolated from peripheral blood of CML (chronic myeloblastic leukemia) patients compared to the dividing cells from normal donors.
DESCRIPTION_FULL	Quiescent and dividing hemopoietic stem cells (HSC) display marked differences in their ability to move between the peripheral circulation and the bone marrow. Specifically, long-term engraftment potential predominantly resides in the quiescent HSC subfraction, and G-CSF mobilization results in the preferential accumulation of quiescent HSC in the periphery. In contrast, stem cells from chronic myeloid leukemia (CML) patients display a constitutive presence in the circulation. To understand the molecular basis for this, we have used microarray technology to analyze the transcriptional differences between dividing and quiescent, normal, and CML-derived CD34+ cells. Our data show a remarkable transcriptional similarity between normal and CML dividing cells, suggesting that the effects of BCR-ABL on the CD34+ cell transcriptome are more limited than previously thought. In addition, we show that quiescent CML cells are more similar to their dividing counterparts than quiescent normal cells are to theirs. We also show these transcriptional differences to be reflected in the altered proliferative activity of normal and CML CD34+ cells. Of the most interest is that the major class of genes that is more abundant in the quiescent cells compared with the dividing cells encodes members of the chemokine family. We propose a role for chemokines expressed by quiescent HSC in the orchestration of CD34+ cell mobilization. Disclosure of potential conflicts of interest is found at the end of this article.
PMID	17717066
GEOID	
AUTHORS	Graham SM,Vass JK,Holyoake TL,Graham GJ
CONTRIBUTOR	Arthur Liberzon
CONTRIBUTOR_ORG	MSigDB Team
EXACT_SOURCE	Table 4S: Ratio NDvCG > 3
FILTERED_BY_SIMILARITY	
EXTERNAL_NAMES_FOR_SIMILAR_TERMS	
EXTERNAL_DETAILS_URL	
SOURCE_MEMBERS	201215_at,203949_at,204304_s_at,206310_at,209480_at,209728_at,210164_at,212560_at,212592_at,212671_s_at,212998_x_at,212999_x_at,213831_at,215051_x_at,219288_at
GENE_SYMBOLS	PLS3,MPO,PROM1,SPINK2,HLA-DQB1,HLA-DRB4,GZMB,SORL1,JCHAIN,HLA-DQA1,HLA-DQB1,HLA-DQB1,HLA-DQA1,AIF1,CEP15
FOUNDER_NAMES