STANDARD_NAME	GSE14000_4H_VS_16H_LPS_DC_TRANSLATED_RNA_UP
SYSTEMATIC_NAME	M3351
COLLECTION	C7:IMMUNESIGDB
MSIGDB_URL	https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/GSE14000_4H_VS_16H_LPS_DC_TRANSLATED_RNA_UP
NAMESPACE	HUMAN_GENE_SYMBOL
DESCRIPTION_BRIEF	Genes up-regulated in comparison of polysome bound (translated) mRNA in dendritic cells (DC) at 4 h after LPS (TLR4 agonist) stimulation versus those at 16 h after the stimulation.
DESCRIPTION_FULL	Dendritic cells (DCs) are the sentinels of the mammalian immune system and they undergo a complex maturation process mediated by activation upon pathogen detection. Recent studies described the analysis of activated DCs by transcriptional profiling, but translation regulation was never taken in account. Therefore, the nature of the mRNAs being translated at various stages of DC activation was determined with the help of translational profiling, which is the sucrose gradient fractionation of polysomal-bound mRNAs combined to microarrays analysis. Total and polysomal-bound mRNA populations were compared in immature (0h) and LPS-stimulated (4h and 16h) human monocyte-derived DCs with the help of Affymetrix microarrays. Biostatistical analysis indicated that 296 mRNA molecules are translationally regulated during DC-activation. The most abundant biological process among the regulated mRNAs was protein biosynthesis, indicating the existence of a negative feedback loop regulating translation. Interestingly, a cluster of 17 ribosomal proteins were part of the regulated mRNAs, indicating that translation may be fine-tuned by particular components of the translational machinery. Our observations highlight the importance of translation regulation during the immune response, and may favour the identification of novel gene clusters or protein networks relevant for immunity. Our study also provides information on the possible absence of correlation between gene expression and real protein production in DCs.
PMID	19943945
GEOID	GSE14000
AUTHORS	Ceppi M,Clavarino G,Gatti E,Schmidt EK,de Gassart A,Blankenship D,Ogola G,Banchereau J,Chaussabel D,Pierre P
CONTRIBUTOR	Jernej Godec
CONTRIBUTOR_ORG	Dana-Farber Cancer Institute
EXACT_SOURCE	GSE14000_1535_200_UP
FILTERED_BY_SIMILARITY	
EXTERNAL_NAMES_FOR_SIMILAR_TERMS	
EXTERNAL_DETAILS_URL	
SOURCE_MEMBERS	AAK1,ABAT,ABCC4,ABCG2,ABHD2,ABL2,ACE,ACTN1,AGL,AHCY,ALDH6A1,ARHGAP24,ARHGAP26,ARHGDIB,ASAP1,ASPHD2,ATF5,ATM,ATP11B,ATP13A3,ATP1B1,ATP8A1,BLVRB,BLZF1,BPNT1,BRWD3,BTG2,BTK,C10orf11,C11orf51,C14orf139,C16orf7,C1orf162,C3orf1,C6orf108,CATSPER2,CBX7,CD93,CDC42EP3,CECR6,CELA2A,CLDN1,CLEC10A,CLEC4G,CLN3,CMTM3,CTNND1,CXCR1,CYB5R1,CYFIP1,CYP1B1,DNASE1L1,DNASE1L3,DOCK2,DTX4,EIF2C2,EIF3K,EMB,EPAS1,ERMP1,ERRFI1,ESYT1,EVL,EXOSC5,F13A1,FAM168B,FAM198B,FBP1,FXYD5,GALM,GAS7,GCLC,GLIPR1,GMFG,GPD1L,GRAMD3,GRHL1,GSN,GSTT1,HADHA,HADHB,HOMER1,HSD17B10,HTT,HUWE1,ICAM3,IL17RA,IL1R2,IL1RAP,ITGAM,ITGB5,JAZF1,KRT17,LAMC1,LMNA,LOC100133660,LOC100287182,LOC285628,LOC400590,LOC401074,MAP1S,MAP2K3,MAP4K1,MBNL1,MEGF9,MESDC1,MGMT,MGST2,MIR101-1,MKNK1,MS4A6A,MSRA,MYO1E,NAGA,NCF2,NCOA7,NDUFAF2,NDUFB6,NFKBIZ,NLN,NLRP1,NRP1,NXN,P2RY6,PARVG,PCYT1A,PEAK1,PECAM1,PEMT,PGK2,PKIB,PLA2G4A,PLAT,PLEKHO2,PLS3,PLXDC2,PON2,PPARD,PPT1,PRKD3,PRKX,PS1TP4,PTGS1,PTPLB,PTX3,PYCARD,PYROXD2,QPRT,RAB7B,RARA,RFWD2,RGS10,RIN3,RNASE6,RPL24,RPL27,RPS4Y1,RPS9,RXRA,S100A4,SCARB1,SDF2L1,SGMS2,SH3GL1,SKAP2,SLC2A3,SLC38A6,SLC47A1,SNX29,SPCS3,SRSF6,STX12,TACC1,TAPT1,TARBP1,TLE3,TM6SF1,TMC7,TMEM194A,TMEM45B,TNC,TPMT,TRAF3,TRPS1,TSC1,TSPAN32,TTC3,TTC39B,UBA52,UBASH3B,UGGT1,UQCRB,USP9X,WWC3,YIF1B,ZC3H12C,ZC3HAV1,ZFP106,ZNF501,ZNF710
GENE_SYMBOLS	AAK1,ABAT,ABCC4,ABCG2,ABHD2,ABL2,ACE,ACTN1,AGL,AHCY,ALDH6A1,ARHGAP24,ARHGAP26,ARHGDIB,ASAP1,ASPHD2,ATF5,ATM,ATP11B,ATP13A3,ATP1B1,ATP8A1,BLVRB,BLZF1,BPNT1,BRWD3,BTG2,BTK,LRMDA,ANAPC15,SYNE3,VPS9D1,C1orf162,TIMMDC1,DNPH1,CATSPER2,CBX7,CD93,CDC42EP3,TMEM121B,CELA2A,CLDN1,CLEC10A,CLEC4G,CLN3,CMTM3,CTNND1,CXCR1,CYB5R1,CYFIP1,CYP1B1,DNASE1L1,DNASE1L3,DOCK2,DTX4,AGO2,EIF3K,EMB,EPAS1,ERMP1,ERRFI1,ESYT1,EVL,EXOSC5,F13A1,FAM168B,GASK1B,FBP1,FXYD5,GALM,GAS7,GCLC,GLIPR1,GMFG,GPD1L,GRAMD2B,GRHL1,GSN,GSTT1,HADHA,HADHB,HOMER1,HSD17B10,HTT,HUWE1,ICAM3,IL17RA,IL1R2,IL1RAP,ITGAM,ITGB5,JAZF1,KRT17,LAMC1,LMNA,,,MIR3142HG,,LINC00960,MAP1S,MAP2K3,MAP4K1,MBNL1,MEGF9,TLNRD1,MGMT,MGST2,MIR101-1,MKNK1,MS4A6A,MSRA,MYO1E,NAGA,NCF2,NCOA7,NDUFAF2,NDUFB6,NFKBIZ,NLN,NLRP1,NRP1,NXN,P2RY6,PARVG,PCYT1A,PEAK1,PECAM1,PEMT,PGK2,PKIB,PLA2G4A,PLAT,PLEKHO2,PLS3,PLXDC2,PON2,PPARD,PPT1,PRKD3,PRKX,,PTGS1,HACD2,PTX3,PYCARD,PYROXD2,QPRT,RAB7B,RARA,COP1,RGS10,RIN3,RNASE6,RPL24,RPL27,RPS4Y1,RPS9,RXRA,S100A4,SCARB1,SDF2L1,SGMS2,SH3GL1,SKAP2,SLC2A3,SLC38A6,SLC47A1,SNX29,SPCS3,SRSF6,STX12,TACC1,TAPT1,TARBP1,TLE3,TM6SF1,TMC7,NEMP1,TMEM45B,TNC,TPMT,TRAF3,TRPS1,TSC1,TSPAN32,TTC3,TTC39B,UBA52,UBASH3B,UGGT1,UQCRB,USP9X,WWC3,YIF1B,ZC3H12C,ZC3HAV1,ZNF106,ZNF501,ZNF710
FOUNDER_NAMES