STANDARD_NAME	GSE19198_CTRL_VS_IL21_TREATED_TCELL_6H_UP
SYSTEMATIC_NAME	M7221
COLLECTION	C7:IMMUNESIGDB
MSIGDB_URL	https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/GSE19198_CTRL_VS_IL21_TREATED_TCELL_6H_UP
NAMESPACE	HUMAN_GENE_SYMBOL
DESCRIPTION_BRIEF	Genes up-regulated in T cells: control (0h) versus IL21 [GeneID=59067] treatment for 6h.
DESCRIPTION_FULL	Interleukin-21 (IL-21) is a pleiotropic cytokine that induces expression of transcription factor BLIMP1 (encoded by Prdm1), which regulates plasma cell differentiation and T cell homeostasis. We identified an IL-21 response element downstream of Prdm1 that binds the transcription factors STAT3 and IRF4, which are required for optimal Prdm1 expression. Genome-wide ChIP-Seq mapping of STAT3- and IRF4-binding sites showed that most regions with IL-21-induced STAT3 binding also bound IRF4 in vivo, and furthermore, revealed that the noncanonical TTCnnnTAA GAS motif critical in Prdm1 was broadly used for STAT3 binding. Comparing genome-wide expression array data to binding sites revealed that most IL-21-regulated genes were associated with combined STAT3-IRF4 sites rather than pure STAT3 sites. Correspondingly, ChIP-Seq analysis of Irf4_/_ T cells showed greatly diminished STAT3 binding after IL-21 treatment, and Irf4_/_ mice showed impaired IL- 21-induced Tfh cell differentiation in vivo. These results reveal broad cooperative gene regulation by STAT3 and IRF4.
PMID	20064451
GEOID	GSE19198
AUTHORS	Kwon H,Thierry-Mieg D,Thierry-Mieg J,Kim HP,Oh J,Tunyaplin C,Carotta S,Donovan CE,Goldman ML,Tailor P,Ozato K,Levy DE,Nutt SL,Calame K,Leonard WJ
CONTRIBUTOR	Jernej Godec
CONTRIBUTOR_ORG	Dana-Farber Cancer Institute
EXACT_SOURCE	GSE19198_2365_200_UP
FILTERED_BY_SIMILARITY	
EXTERNAL_NAMES_FOR_SIMILAR_TERMS	
EXTERNAL_DETAILS_URL	
SOURCE_MEMBERS	ADAT2,ADCK3,AFF1,AHCY,AIF1,AMICA1,ANKS1A,ARAP2,ARHGAP4,ARL2,ATM,ATP5B,ATP5D,ATP8B4,BCAR3,BIRC3,BLVRA,BLVRB,BTF3L4,BZW2,C14orf159,C17orf90,C17orf96,C3orf21,C6orf108,C9orf72,CAPNS1,CBR3,CCDC85B,CCL13,CCL23,CCT3,CD209,CD33,CD48,CDC123,CDK6,CHST15,CLIP2,CMAHP,CORO1A,CSF2RB,CUL9,CXCL2,CYC1,CYSLTR1,CYTH4,DAGLA,DAP,DARS,DCLRE1B,DCTPP1,DHRS9,DTD1,EHD1,EHD4,EMB,EMILIN2,ENTPD6,EXT1,FAR2,FCER2,FCGR2B,FIGNL1,FIS1,FLI1,FNBP1,FRMD4B,GALK1,GATM,GGTA1P,GIMAP5,GINS1,GMFG,GNAI2,GPI,HCST,HLX,HMHA1,HN1L,HRH1,HSPA6,IDH3G,IFI16,IFITM2,IFITM3,IL10RA,IL18BP,IL2RG,IMP4,INPP5D,IPO4,ITSN1,JUP,KIAA0889,LILRB2,LRRC25,LRRC33,MAP3K1,MARCH1,MCM6,METRNL,MGRN1,MIF,MLLT6,MOBKL1B,MOBKL2C,MPEG1,MPP1,MRPL15,MRPL24,MYC,NAIP,NAMPT,NCF4,NDUFV1,NECAP2,NHP2L1,NME1,NOB1,NRP2,NUCKS1,OAS2,OBFC2A,OGDH,OXCT1,P2RY12,P2RY13,P2RY14,PAPSS2,PARL,PDCD4,PDLIM2,PFKM,PFKP,PHTF2,PIGX,PIK3R6,PLEK,POLD2,PPARGC1B,PPP2R4,PRDX4,PRKACB,PRMT1,PRSS23,QTRT1,RAB33A,RAN,RBPJ,RCSD1,RNF125,RNF128,RNF144B,RUVBL1,SCARB1,SCFD2,SECTM1,SELPLG,SETD7,SFXN4,SIGLEC1,SLC16A7,SLC25A12,SLC25A5,SLC46A3,SLCO3A1,SLITRK4,SNAP23,SNX6,SRI,SRM,ST3GAL5,SUCLG1,SULF2,SYNE1,TBC1D14,TBC1D2B,TBCB,TCF4,THOC1,TMEM173,TMOD2,TNFAIP2,TNFRSF1A,TNIP1,TRAF3,TRAP1,TRERF1,TRIM32,TSPAN4,TTYH2,TUBA1A,TUBB,TXN2,UBAC1,UROS,VARS,WASF2,XPO6
GENE_SYMBOLS	ADAT2,COQ8A,AFF1,AHCY,AIF1,JAML,ANKS1A,ARAP2,ARHGAP4,ARL2,ATM,ATP5F1B,ATP5F1D,ATP8B4,BCAR3,BIRC3,BLVRA,BLVRB,BTF3L4,BZW2,DGLUCY,OXLD1,EPOP,XXYLT1,DNPH1,C9orf72,CAPNS1,CBR3,CCDC85B,CCL13,CCL23,CCT3,CD209,CD33,CD48,CDC123,CDK6,CHST15,CLIP2,CMAHP,CORO1A,CSF2RB,CUL9,CXCL2,CYC1,CYSLTR1,CYTH4,DAGLA,DAP,DARS1,DCLRE1B,DCTPP1,DHRS9,DTD1,EHD1,EHD4,EMB,EMILIN2,ENTPD6,EXT1,FAR2,FCER2,FCGR2B,FIGNL1,FIS1,FLI1,FNBP1,FRMD4B,GALK1,GATM,GGTA1,GIMAP5,GINS1,GMFG,GNAI2,GPI,HCST,HLX,ARHGAP45,JPT2,HRH1,HSPA6,IDH3G,IFI16,IFITM2,IFITM3,IL10RA,IL18BP,IL2RG,IMP4,INPP5D,IPO4,ITSN1,JUP,MTCL2,LILRB2,LRRC25,NRROS,MAP3K1,MARCHF1,MCM6,METRNL,MGRN1,MIF,MLLT6,MOB1A,MOB3C,MPEG1,MPP1,MRPL15,MRPL24,MYC,NAIP,NAMPT,NCF4,NDUFV1,NECAP2,SNU13,NME1,NOB1,NRP2,NUCKS1,OAS2,NABP1,OGDH,OXCT1,P2RY12,P2RY13,P2RY14,PAPSS2,PARL,PDCD4,PDLIM2,PFKM,PFKP,PHTF2,PIGX,PIK3R6,PLEK,POLD2,PPARGC1B,PTPA,PRDX4,PRKACB,PRMT1,PRSS23,QTRT1,RAB33A,RAN,RBPJ,RCSD1,RNF125,RNF128,RNF144B,RUVBL1,SCARB1,SCFD2,SECTM1,SELPLG,SETD7,SFXN4,SIGLEC1,SLC16A7,SLC25A12,SLC25A5,SLC46A3,SLCO3A1,SLITRK4,SNAP23,SNX6,SRI,SRM,ST3GAL5,SUCLG1,SULF2,SYNE1,TBC1D14,TBC1D2B,TBCB,TCF4,THOC1,STING1,TMOD2,TNFAIP2,TNFRSF1A,TNIP1,TRAF3,TRAP1,TRERF1,TRIM32,TSPAN4,TTYH2,TUBA1A,TUBB,TXN2,UBAC1,UROS,VARS1,WASF2,XPO6
FOUNDER_NAMES