STANDARD_NAME GSE22103_UNSTIM_VS_LPS_STIM_NEUTROPHIL_UP SYSTEMATIC_NAME M7794 COLLECTION C7:IMMUNESIGDB MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/GSE22103_UNSTIM_VS_LPS_STIM_NEUTROPHIL_UP NAMESPACE HUMAN_GENE_SYMBOL DESCRIPTION_BRIEF Genes up-regulated in neutrophils: untreated versus LPS. DESCRIPTION_FULL Neutrophils play critical roles in modulating the immune response. However, neutrophils have a short circulating half life, are readily stimulated in vitro, and have low levels of cellular mRNA when compared to other blood leukocyte populations. All of these factors have made it difficult to evaluate neutrophils from clinical populations for molecular and functional studies. Here we present a robust methodology for rapidly isolating neutrophils directly from whole blood and develop ‘on- chip’ processing for mRNA and protein isolation for genomics and proteomics. We validate this device with an ex vivo stimulation experiment and demonstrate the ability of the device to discriminate subtle differences in the genomic and proteomic response of peripheral blood neutrophils to direct and indirect stimulation. Lastly, we implement this tool as part of a near patient blood processing system within a multi-center clinical study of the immune response to severe trauma and burn injury and demonstrate that this technique is easy to use by nurses and technical staff yielding excellent quality and sufficient quantity of mRNA for sensitive genomic readout of the host response to injury PMID 20802500 GEOID GSE22103 AUTHORS Kotz KT,Xiao W,Miller-Graziano C,Qian WJ,Russom A,Warner EA,Moldawer LL,De A,Bankey PE,Petritis BO,Camp DG 2nd,Rosenbach AE,Goverman J,Fagan SP,Brownstein BH,Irimia D,Xu W,Wilhelmy J,Mindrinos MN,Smith RD,Davis RW,Tompkins RG,Toner M,Inflammation and the Host Response to Injury Collaborative Research Program CONTRIBUTOR Jernej Godec CONTRIBUTOR_ORG Dana-Farber Cancer Institute EXACT_SOURCE GSE22103_3647_200_UP FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS ABHD2,ABI3,ADAM8,AEN,AKAP1,AMY2A,AMZ2,ANAPC4,ANKRD39,ANXA5,AQR,ARCN1,ARFGAP3,ARPC5L,ASB2,ATF1,BAG5,BBS5,BCL2A1,BCL3,BDP1,BEND3,BET1L,BRWD1,C14orf118,C1orf38,C7orf25,C8orf33,C9orf23,C9orf3,CAMK2N2,CCDC71,CCDC90B,CD40,CDC34,CDK13,CEBPA,CLK3,CRK,CRTC2,CTU1,DCP1A,DDIT3,DDX51,DEF6,DNAJC30,EIF1B,ELAC2,ERCC1,ERRFI1,EXOSC3,FAIM,FAM105B,FAM122A,FAM129B,FANCF,FAS,FBXL14,FNBP1,FOXJ3,GFER,GNA13,GRIPAP1,GTPBP4,HAPLN3,HBS1L,HIVEP1,HIVEP3,HS6ST1,ICAM1,IFNAR1,IL4R,IL7R,IMP3,INTS6,IPO7,IRF2BPL,IRF4,ITGA5,ITPKC,IWS1,JUNB,KCNN4,KIAA0247,KIF2A,KLF16,KLHL26,KLHL9,LFNG,LRFN4,LSM12,MAGOHB,MAP2K4,MAPKAPK3,MARK4,MAX,MBLAC1,MFHAS1,MFSD5,MINA,MLLT6,MMADHC,MMP12,MORF4L1,MRPL17,MRPL22,MRPS18B,NAA16,NDNL2,NECAP1,NELF,NFE2L2,NFIC,NINJ1,NIP7,NOL10,NPLOC4,NR1H2,NRF1,NSRP1,NUB1,NUP35,OPTN,OSGIN1,OTUD4,OVCA2,PDGFB,PHLDA1,PHRF1,PIGY,PIK3R5,PIM3,PIP5K1C,PPP1R15B,PPP1R16B,PPP2CA,PRDM2,PRMT6,PRR13,PSMG3,PTPN9,PTX3,RABGGTB,RARA,RASGEF1B,RDH13,RELA,RGP1,RGS1,RHOC,RHOG,RNASEH1,RNF11,RPS19BP1,SDHAF1,SEC22A,SECISBP2,SEMA4D,SENP3,SETD5,SF3A2,SH3PXD2B,SKIL,SLC7A1,SLMO2,SMCR8,SOCS5,SPIN4,ST6GALNAC4,TAF1A,THUMPD1,TIMM10,TM7SF4,TNFAIP2,TP53RK,TRAF3,TRIB1,TRMT61A,TUBA1A,TUBB6,UBE2I,UBOX5,UBXN4,USP42,VASP,WDR1,WDR75,YEATS4,ZBTB39,ZDHHC18,ZEB1,ZFP36L1,ZFP64,ZFYVE20,ZNF192,ZNF280C,ZNF282,ZNF295,ZNF639,ZNF841 GENE_SYMBOLS ABHD2,ABI3,ADAM8,AEN,AKAP1,AMY2A,AMZ2,ANAPC4,ANKRD39,ANXA5,AQR,ARCN1,ARFGAP3,ARPC5L,ASB2,ATF1,BAG5,BBS5,BCL2A1,BCL3,BDP1,BEND3,BET1L,BRWD1,GPATCH2L,THEMIS2,C7orf25,C8orf33,RPP25L,AOPEP,CAMK2N2,CCDC71,CCDC90B,CD40,CDC34,CDK13,CEBPA,CLK3,CRK,CRTC2,CTU1,DCP1A,DDIT3,DDX51,DEF6,DNAJC30,EIF1B,ELAC2,ERCC1,ERRFI1,EXOSC3,FAIM,OTULIN,PABIR1,NIBAN2,FANCF,FAS,FBXL14,FNBP1,FOXJ3,GFER,GNA13,GRIPAP1,GTPBP4,HAPLN3,HBS1L,HIVEP1,HIVEP3,HS6ST1,ICAM1,IFNAR1,IL4R,IL7R,IMP3,INTS6,IPO7,IRF2BPL,IRF4,ITGA5,ITPKC,IWS1,JUNB,KCNN4,SUSD6,KIF2A,KLF16,KLHL26,KLHL9,LFNG,LRFN4,LSM12,MAGOHB,MAP2K4,MAPKAPK3,MARK4,MAX,MBLAC1,MFHAS1,MFSD5,RIOX2,MLLT6,MMADHC,MMP12,MORF4L1,MRPL17,MRPL22,MRPS18B,NAA16,NSMCE3,NECAP1,NSMF,NFE2L2,NFIC,NINJ1,NIP7,NOL10,NPLOC4,NR1H2,NRF1,NSRP1,NUB1,NUP35,OPTN,OSGIN1,OTUD4,OVCA2,PDGFB,PHLDA1,PHRF1,PIGY,PIK3R5,PIM3,PIP5K1C,PPP1R15B,PPP1R16B,PPP2CA,PRDM2,PRMT6,PRR13,PSMG3,PTPN9,PTX3,RABGGTB,RARA,RASGEF1B,RDH13,RELA,RGP1,RGS1,RHOC,RHOG,RNASEH1,RNF11,RPS19BP1,SDHAF1,SEC22A,SECISBP2,SEMA4D,SENP3,SETD5,SF3A2,SH3PXD2B,SKIL,SLC7A1,PRELID3B,SMCR8,SOCS5,SPIN4,ST6GALNAC4,TAF1A,THUMPD1,TIMM10,DCSTAMP,TNFAIP2,TP53RK,TRAF3,TRIB1,TRMT61A,TUBA1A,TUBB6,UBE2I,UBOX5,UBXN4,USP42,VASP,WDR1,WDR75,YEATS4,ZBTB39,ZDHHC18,ZEB1,ZFP36L1,ZFP64,RBSN,ZKSCAN8,ZNF280C,ZNF282,ZBTB21,ZNF639,ZNF841 FOUNDER_NAMES