STANDARD_NAME GSE2405_HEAT_KILLED_LYSATE_VS_LIVE_A_PHAGOCYTOPHILUM_STIM_NEUTROPHIL_9H_UP SYSTEMATIC_NAME M6198 COLLECTION C7:IMMUNESIGDB MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/GSE2405_HEAT_KILLED_LYSATE_VS_LIVE_A_PHAGOCYTOPHILUM_STIM_NEUTROPHIL_9H_UP NAMESPACE HUMAN_GENE_SYMBOL DESCRIPTION_BRIEF Genes up-regulated in polymorphonuclear leukocytes (9h): treated by heat killed HC60 cell (promyelocytic leukemia) lysate versus A. phagocytophilum infection. DESCRIPTION_FULL Polymorphonuclear leukocytes (PMNs) were obtained from healthy individuals in accordance with protocols approved by the Institutional Review Board for Human Subjects at the University of Minnesota and the National Institute of Allergy and Infectious Diseases. PMNs (107) were combined on ice with live S. aureus (108) or with live or heat-killed A. phagocytophilum (bacteria isolated from 5x106 infected HL60 cells for a ratio of 1 infected HL60 cell: 2 PMNs, ~ 5-20 A. phagocytophilum: PMN) in wells of a 12-well tissue culture plate (pre-coated with 20% autologous normal human serum). Unstimulated control assays received either buffer (for S. aureus comparisons) or clarified HL60 lysate (for A. phagocytophilum comparisons). Plates were centrifuged at 350 x g for 8 min at 4oC to synchronize phagocytosis and incubated at 37 deg. C in a CO2 incubator for the indicated times. At the indicated times, tissue culture medium was aspirated from the plate and PMNs were lysed directly with RLT buffer (Qiagen, Valencia, CA). Purification of PMN RNA and subsequent preparation of labeled cRNA target was performed as described in Methods. Labeling of samples, hybridization of cRNA with HU133A oligonucleotide arrays (Affymetrix, Santa Clara, CA), and scanning were performed according to standard Affymetrix protocols ( http://www.affymetrix.com/pdf/expression_manual.pdf ). Experiments were performed in triplicate, using PMNs from three healthy individuals for each treatment. PMID 15879137 GEOID GSE2405 AUTHORS Borjesson DL,Kobayashi SD,Whitney AR,Voyich JM,Argue CM,Deleo FR CONTRIBUTOR Jernej Godec CONTRIBUTOR_ORG Dana-Farber Cancer Institute EXACT_SOURCE GSE2405_3590_200_UP FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS ADA,ADAM12,AGFG2,AKAP2,ALDH7A1,ANGPTL2,ANKRD50,ANXA2,AQP11,ARPP21,ASF1B,ASPM,ASRGL1,AURKA,BANK1,BID,BUB1B,C10orf27,C11orf74,C12orf75,C15orf23,C15orf42,C3,CAMKK1,CCNB2,CD83,CD8A,CD93,CDC20,CDC25C,CDC45,CDC6,CDCA5,CDCA8,CDK2AP1,CDKN2C,CENPE,CENPF,CERK,CHCHD6,CKAP2,CKAP2L,CKS2,CLIC4,CLK3,COL4A1,CRIP2,CSDA,CSF1R,CSTB,CTSH,CTSL2,CXCL12,CXCR4,CYB5A,DAB2IP,DEPDC1B,DGKE,DHRS3,DIRAS2,DMRT2,DNAJC6,DNTT,DSTN,DUT,DYNLT1,E2F7,ENDOU,EPB41L3,ETS2,EXO1,FABP5,FAM57A,FBXL12,FCER1G,FEN1,FGR,FKBP5,FOXN1,GABARAPL1,GFI1,GLCCI1,GMPR,GPR56,GTF2H4,HEMGN,HEY1,HLA-DQA1,HLA-DRB1,HMGB2,HMGB3,HMMR,HPGD,HS6ST1,IFNGR1,IGHM,IGKC,IGSF8,IL18,IL7,INMT,ISG20,KIAA0101,KIF11,KIF18B,KIF20A,KIF2C,KPNA2,KRT18,LDHB,LIG1,LIG4,LONRF1,LPCAT2,LRP1,LY75,MAD2L1,MAGED1,MED13,MIER1,MKI67,MMP2,MNS1,MORC1,MPP1,MR1,MREG,MXD3,NCK2,NFATC3,NIM1,NPNT,NTN1,NUF2,OCIAD2,ODZ4,OSBPL1A,PAPSS2,PBK,PKP1,PLEKHB1,PLTP,PMM1,POU6F1,PPPDE2,PRC1,PRSS16,PSAP,PSMB11,PTPN13,RAG1,RAG2,RAPGEF3,RCBTB2,RMND5A,RPA3,RPS27L,RRM2,SAP30,SCN2B,SFRP1,SGOL1,SGPL1,SH2D1A,SKA1,SLC6A19,SLC6A8,SMARCC2,SMARCD2,SNAP91,SPATS2,SPC24,SPC25,SPO11,SSBP2,STMN1,STOM,SYK,SYT13,SYTL3,TCF4,TCF7,TF,TMEM107,TMEM140,TMEM151B,TMEM51,TNC,TP53INP2,TSC22D1,TUBA1B,TUBE1,TXN,WDR91,WEE1,WHSC1,WNT4,XRCC6,ZNF503,ZNF704 GENE_SYMBOLS ADA,ADAM12,AGFG2,,ALDH7A1,ANGPTL2,ANKRD50,ANXA2,AQP11,ARPP21,ASF1B,ASPM,ASRGL1,AURKA,BANK1,BID,BUB1B,TBATA,IFTAP,C12orf75,KNSTRN,TICRR,C3,CAMKK1,CCNB2,CD83,CD8A,CD93,CDC20,CDC25C,CDC45,CDC6,CDCA5,CDCA8,CDK2AP1,CDKN2C,CENPE,CENPF,CERK,CHCHD6,CKAP2,CKAP2L,CKS2,CLIC4,CLK3,COL4A1,CRIP2,YBX3,CSF1R,CSTB,CTSH,CTSV,CXCL12,CXCR4,CYB5A,DAB2IP,DEPDC1B,DGKE,DHRS3,DIRAS2,DMRT2,DNAJC6,DNTT,DSTN,DUT,DYNLT1,E2F7,ENDOU,EPB41L3,ETS2,EXO1,FABP5,TLCD3A,FBXL12,FCER1G,FEN1,FGR,FKBP5,FOXN1,GABARAPL1,GFI1,GLCCI1,GMPR,ADGRG1,GTF2H4,HEMGN,HEY1,HLA-DQA1,HLA-DRB1,HMGB2,HMGB3,HMMR,HPGD,HS6ST1,IFNGR1,IGHM,IGKC,IGSF8,IL18,IL7,INMT,ISG20,PCLAF,KIF11,KIF18B,KIF20A,KIF2C,KPNA2,KRT18,LDHB,LIG1,LIG4,LONRF1,LPCAT2,LRP1,LY75,MAD2L1,MAGED1,MED13,MIER1,MKI67,MMP2,MNS1,MORC1,MPP1,MR1,MREG,MXD3,NCK2,NFATC3,NIM1K,NPNT,NTN1,NUF2,OCIAD2,TENM4,OSBPL1A,PAPSS2,PBK,PKP1,PLEKHB1,PLTP,PMM1,POU6F1,DESI1,PRC1,PRSS16,PSAP,PSMB11,PTPN13,RAG1,RAG2,RAPGEF3,RCBTB2,RMND5A,RPA3,RPS27L,RRM2,SAP30,SCN2B,SFRP1,SGO1,SGPL1,SH2D1A,SKA1,SLC6A19,SLC6A8,SMARCC2,SMARCD2,SNAP91,SPATS2,SPC24,SPC25,SPO11,SSBP2,STMN1,STOM,SYK,SYT13,SYTL3,TCF4,TCF7,TF,TMEM107,TMEM140,TMEM151B,TMEM51,TNC,TP53INP2,TSC22D1,TUBA1B,TUBE1,TXN,WDR91,WEE1,NSD2,WNT4,XRCC6,ZNF503,ZNF704 FOUNDER_NAMES