STANDARD_NAME	GSE36888_UNTREATED_VS_IL2_TREATED_STAT5_AB_KNOCKIN_TCELL_6H_UP
SYSTEMATIC_NAME	M8737
COLLECTION	C7:IMMUNESIGDB
MSIGDB_URL	https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/GSE36888_UNTREATED_VS_IL2_TREATED_STAT5_AB_KNOCKIN_TCELL_6H_UP
NAMESPACE	HUMAN_GENE_SYMBOL
DESCRIPTION_BRIEF	Genes up-regulated in STAT5 double knock-in T cells: control versus IL2 [GeneID=3558] stimulation for 6h.
DESCRIPTION_FULL	Cytokine-activated STAT proteins dimerize and bind to high-affinity motifs, and N-terminal domain-mediated oligomerization of dimers allows tetramer formation and binding to low-affinity tandem motifs, but the functions of dimers versus tetramers are unknown. We generated Stat5a and Stat5b double knock-in (DKI) N-domain mutant mice that form dimers but not tetramers, identified cytokine-regulated genes whose expression required STAT5 tetramers, and defined consensus motifs for dimers versus tetramers. Whereas Stat5- deficient mice exhibited perinatal lethality, DKI mice were viable, indicating that STAT5 dimers were sufficient for survival. Nevertheless, STAT5 DKI mice had fewer CD4+CD25+ T cells, NK cells, and CD8+ T cells, with impaired cytokine-induced proliferation and homeostatic proliferation of CD8+ T cells. DKI CD8+ T cell proliferation following viral infection was diminished and DKI Treg cells did not efficiently control colitis. Thus, tetramerization of STAT5 is dispensable for survival but is critical for cytokine responses and normal immune function.
PMID	22520852
GEOID	GSE36888
AUTHORS	Lin JX,Li P,Liu D,Jin HT,He J,Ata Ur Rasheed M,Rochman Y,Wang L,Cui K,Liu C,Kelsall BL,Ahmed R,Leonard WJ
CONTRIBUTOR	Jernej Godec
CONTRIBUTOR_ORG	Dana-Farber Cancer Institute
EXACT_SOURCE	GSE36888_2356_200_UP
FILTERED_BY_SIMILARITY	
EXTERNAL_NAMES_FOR_SIMILAR_TERMS	
EXTERNAL_DETAILS_URL	
SOURCE_MEMBERS	AKAP9,ANKRD13D,ANO10,APH1B,ARHGAP19,ASCL1,ASPHD2,ATP7B,BEST1,BST1,BTBD10,C12orf61,C1orf115,C20orf160,C2CD3,C3orf70,C6orf211,C8orf60,CA13,CAB39,CASP5,CCL19,CD14,CD209,CD47,CDC42EP5,CEP135,CHCHD7,CISH,CLEC4G,CLIC1,CORO1A,CPD,CRLF3,CYP3A5,DDIT4,DKFZP434I0714,DMRT1,EDEM2,ELL,EMILIN2,EMR3,ESRRG,ETV3,FAM126A,FAM49A,FAM73B,FAM84A,FCGR2A,FCGR2B,FCGR2C,FCRLB,FIGNL1,FLJ34208,FPR1,FPR2,FSD1L,GK5,GLCE,GLDC,GNG2,GPR83,GRB2,GSTA4,GUSBP3,HAS1,HCK,HRH1,HS3ST3B1,HTR7,IER3,IER5,IFT27,IFT43,IL1B,IL6ST,INSIG2,IQUB,ISX,ITGB8,JAM2,KIAA1211,KSR1,LAT2,LHFPL1,LILRA1,LILRA5,LILRA6,LILRB1,LILRB3,LIMK2,LOC100288939,LOC150381,LOC284630,LOC284837,LOC285628,LOC285957,LOC286135,LOC401312,LOC541472,LOC648987,LOC729178,LRRC25,LRRC4,LYN,MAF,MAGEA1,MAP3K4,MAP3K5,MB21D2,MBOAT2,MED30,MEGF9,MLLT4,MS4A7,MXD1,MYO1G,N4BP1,NAGS,NAMPT,NCRNA00085,NEFH,NEUROD1,NFE2L1,NFKBIZ,NOD2,OLIG2,P2RY2,P2RY6,PCGF3,PFKFB3,PIK3AP1,PLAC8,PLAGL2,PLK2,PLSCR1,PMP22,PREX1,PRKCD,PRKD3,PTGES,PTPRF,RAB27A,RAB31,RARA,RPGR,RYBP,S100A12,S100A8,S100A9,SAMD4B,SAMSN1,SASH1,SEMA4C,SEPT10,SEPT6,SERPINB9,SESN2,SH3PXD2B,SHOC2,SLC16A10,SLC2A10,SLC41A2,SLC4A8,SLC7A7,SMPDL3A,SOCS1,SOCS2,SOCS3,SOD2,SOS1,SPTLC2,SUSD1,SYT17,TBC1D30,TET2,TGFA,THBS1,TM9SF4,TMEM115,TMEM168,TNFRSF1B,TNIP3,TOP1P2,TRAF3IP2,TRIM13,TRIP10,TRNAU1AP,ULK2,USP3,USP32,VAV1,VDR,VEGFA,VNN2,VNN3,WDR17,WDR64,ZBTB1,ZC3H12A
GENE_SYMBOLS	AKAP9,ANKRD13D,ANO10,APH1B,ARHGAP19,ASCL1,ASPHD2,ATP7B,BEST1,BST1,BTBD10,LINC01465,C1orf115,CCM2L,C2CD3,C3orf70,ARMT1,,CA13,CAB39,CASP5,CCL19,CD14,CD209,CD47,CDC42EP5,CEP135,CHCHD7,CISH,CLEC4G,CLIC1,CORO1A,CPD,CRLF3,CYP3A5,DDIT4,MIR4453HG,DMRT1,EDEM2,ELL,EMILIN2,ADGRE3,ESRRG,ETV3,HYCC1,CYRIA,MIGA2,LRATD1,FCGR2A,FCGR2B,FCGR2C,FCRLB,FIGNL1,ATP13A3-DT,FPR1,FPR2,FSD1L,GK5,GLCE,GLDC,GNG2,GPR83,GRB2,GSTA4,GUSBP3,HAS1,HCK,HRH1,HS3ST3B1,HTR7,IER3,IER5,IFT27,IFT43,IL1B,IL6ST,INSIG2,IQUB,ISX,ITGB8,JAM2,CRACD,KSR1,LAT2,LHFPL1,LILRA1,LILRA5,LILRA6,LILRB1,LILRB3,LIMK2,,,,AATBC,MIR3142HG,,LINC02209,STEAP1B,IL6-AS1,ANXA2R-OT1,STXBP5-AS1,LRRC25,LRRC4,LYN,MAF,MAGEA1,MAP3K4,MAP3K5,MB21D2,MBOAT2,MED30,MEGF9,AFDN,MS4A7,MXD1,MYO1G,N4BP1,NAGS,NAMPT,SPACA6,NEFH,NEUROD1,NFE2L1,NFKBIZ,NOD2,OLIG2,P2RY2,P2RY6,PCGF3,PFKFB3,PIK3AP1,PLAC8,PLAGL2,PLK2,PLSCR1,PMP22,PREX1,PRKCD,PRKD3,PTGES,PTPRF,RAB27A,RAB31,RARA,RPGR,RYBP,S100A12,S100A8,S100A9,SAMD4B,SAMSN1,SASH1,SEMA4C,SEPTIN10,SEPTIN6,SERPINB9,SESN2,SH3PXD2B,SHOC2,SLC16A10,SLC2A10,SLC41A2,SLC4A8,SLC7A7,SMPDL3A,SOCS1,SOCS2,SOCS3,SOD2,SOS1,SPTLC2,SUSD1,SYT17,TBC1D30,TET2,TGFA,THBS1,TM9SF4,TMEM115,TMEM168,TNFRSF1B,TNIP3,,TRAF3IP2,TRIM13,TRIP10,TRNAU1AP,ULK2,USP3,USP32,VAV1,VDR,VEGFA,VNN2,VNN3P,WDR17,WDR64,ZBTB1,ZC3H12A
FOUNDER_NAMES