STANDARD_NAME	GSE43955_1H_VS_10H_ACT_CD4_TCELL_WITH_TGFB_IL6_DN
SYSTEMATIC_NAME	M9675
COLLECTION	C7:IMMUNESIGDB
MSIGDB_URL	https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/GSE43955_1H_VS_10H_ACT_CD4_TCELL_WITH_TGFB_IL6_DN
NAMESPACE	HUMAN_GENE_SYMBOL
DESCRIPTION_BRIEF	Genes down-regulated in CD4 [GeneID=920] T helper cells Th17 treated with TGFB1 and IL6 [GeneID=7040;3569]: 1h versus 10h.
DESCRIPTION_FULL	Despite their enormous importance, the molecular circuits that control the differentiation of Th17 cells remain largely unknown. Recent studies have reconstructed regulatory networks in mammalian cells, but have focused on short-term responses and relied on perturbation approaches that cannot be applied to primary T cells. Here, we develop a systematic strategy – combining transcriptional profiling at high temporal resolution, novel computational algorithms, and innovative nanowire-based tools for performing gene perturbations in primary T cells – to derive and experimentally validate a temporal model of the dynamic regulatory network that controls Th17 differentiation. The network is arranged into two self-reinforcing and mutually antagonistic modules that either suppress or promote Th17 differentiation. The two modules contain 12 novel regulators with no previous implication in Th17 differentiation, which may be essential to maintain the appropriate balance of Th17 and other CD4+ T cell subsets. Overall, our study identifies and validates 39 regulatory factors that are embedded within a comprehensive temporal network and identifies novel drug targets and organizational principles for the differentiation of Th17 cells.
PMID	23467089
GEOID	GSE43955
AUTHORS	Yosef N,Shalek AK,Gaublomme JT,Jin H,Lee Y,Awasthi A,Wu C,Karwacz K,Xiao S,Jorgolli M,Gennert D,Satija R,Shakya A,Lu DY,Trombetta JJ,Pillai MR,Ratcliffe PJ,Coleman ML,Bix M,Tantin D,Park H,Kuchroo VK,Regev A
CONTRIBUTOR	Jernej Godec
CONTRIBUTOR_ORG	Dana-Farber Cancer Institute
EXACT_SOURCE	GSE43955_2432_200_DN
FILTERED_BY_SIMILARITY	
EXTERNAL_NAMES_FOR_SIMILAR_TERMS	
EXTERNAL_DETAILS_URL	
SOURCE_MEMBERS	AAAS,ACTN4,ACVR2B,AGFG1,AGL,AHI1,AIF1,ALG2,ANP32A,ANXA1,APBB2,APOC2,APOE,AZGP1,B4GALT6,BGN,BMPR1B,BSN,C17orf79,C19orf46,C19orf73,CCR5,CCRN4L,CD47,CD52,CD5L,CD72,CDKN1C,CHST2,CLIC4,CMIP,CMTM8,CNN3,COL18A1,COL1A2,COL4A2,COL5A2,CP,CR2,CRABP2,CSDA,CSF3R,CSTF2,CTGF,CTSS,CXCL13,CXCL14,CYFIP1,CYTIP,DBNL,DCK,DCN,DKK3,DPM1,ELAVL4,ENG,FBLN2,FGL2,FJX1,FKBP10,FLNB,FN1,FOLR2,FOXN2,FRMD6,FRRS1,FXYD5,GATM,GJA1,GLRX,GP9,GPD2,GPN1,GTPBP1,GTPBP4,H19,H1F0,HELLS,HGSNAT,HLA-E,HLX,IFI35,IFIT3,IGF2BP2,IGFBP2,IL10,IL1B,IL1RL1,IMPA1,IRF7,ITGA8,ITGAE,KAT2A,KIF5C,KLF10,KLRG1,LGALS3BP,LTBP2,LY6E,LY86,LY9,LYN,MED27,MLH1,MLL,MORC4,MYF6,MYO1C,MYO1E,NADK,NAPSA,NCF4,NDUFS6,NECAP1,NEU1,NHP2,NMB,NNMT,NR4A1,OLR1,OR2C1,PDCD1,PDGFB,PDK3,PGK2,PJA1,PLAC8,PLAC9,PLK4,PLS3,PLTP,POU1F1,PREB,PRKAR1A,PRSS8,PSMA7,PTGES2,PTPN5,PXK,RARS2,RBM15,RCAN1,RDH5,RELB,RGL2,RHD,RING1,RNMTL1,RPIA,RRM2,RTN4,S100A11,SBF2,SEMA4D,SERPINE2,SF3B5,SIX1,SLC23A3,SLC35D1,SLC39A6,SLC3A1,SLC6A18,SLC7A8,SNHG6,SOD2,SRPRB,ST8SIA3,STXBP3,SULT4A1,TAGLN2,TAL1,TAP1,TARS2,TBP,TBX6,TFAP2A,TIMP3,TMED10,TMEM176A,TMEM183A,TMEM66,TOP3A,TSPAN13,TSSK1B,TUBB2A,TUBB6,TXNRD1,UBE2L3,UNC5C,USP18,VNN2,VPS26A,WDR20,YES1,ZC3H12C,ZFAND5,ZFPM1,ZNF146,ZNF532,ZRANB1
GENE_SYMBOLS	AAAS,ACTN4,ACVR2B,AGFG1,AGL,AHI1,AIF1,ALG2,ANP32A,ANXA1,APBB2,APOC2,APOE,AZGP1,B4GALT6,BGN,BMPR1B,BSN,COPRS,SYNE4,C19orf73,CCR5,NOCT,CD47,CD52,CD5L,CD72,CDKN1C,CHST2,CLIC4,CMIP,CMTM8,CNN3,COL18A1,COL1A2,COL4A2,COL5A2,CP,CR2,CRABP2,YBX3,CSF3R,CSTF2,CCN2,CTSS,CXCL13,CXCL14,CYFIP1,CYTIP,DBNL,DCK,DCN,DKK3,DPM1,ELAVL4,ENG,FBLN2,FGL2,FJX1,FKBP10,FLNB,FN1,FOLR2,FOXN2,FRMD6,FRRS1,FXYD5,GATM,GJA1,GLRX,GP9,GPD2,GPN1,GTPBP1,GTPBP4,H19,H1-0,HELLS,HGSNAT,HLA-E,HLX,IFI35,IFIT3,IGF2BP2,IGFBP2,IL10,IL1B,IL1RL1,IMPA1,IRF7,ITGA8,ITGAE,KAT2A,KIF5C,KLF10,KLRG1,LGALS3BP,LTBP2,LY6E,LY86,LY9,LYN,MED27,MLH1,KMT2A,MORC4,MYF6,MYO1C,MYO1E,NADK,NAPSA,NCF4,NDUFS6,NECAP1,NEU1,NHP2,NMB,NNMT,NR4A1,OLR1,OR2C1,PDCD1,PDGFB,PDK3,PGK2,PJA1,PLAC8,PLAC9,PLK4,PLS3,PLTP,POU1F1,PREB,PRKAR1A,PRSS8,PSMA7,PTGES2,PTPN5,PXK,RARS2,RBM15,RCAN1,RDH5,RELB,RGL2,RHD,RING1,MRM3,RPIA,RRM2,RTN4,S100A11,SBF2,SEMA4D,SERPINE2,SF3B5,SIX1,SLC23A3,SLC35D1,SLC39A6,SLC3A1,SLC6A18,SLC7A8,SNHG6,SOD2,SRPRB,ST8SIA3,STXBP3,SULT4A1,TAGLN2,TAL1,TAP1,TARS2,TBP,TBX6,TFAP2A,TIMP3,TMED10,TMEM176A,TMEM183A,SARAF,TOP3A,TSPAN13,TSSK1B,TUBB2A,TUBB6,TXNRD1,UBE2L3,UNC5C,USP18,VNN2,VPS26A,WDR20,YES1,ZC3H12C,ZFAND5,ZFPM1,ZNF146,ZNF532,ZRANB1
FOUNDER_NAMES