STANDARD_NAME GSE6092_IFNG_VS_IFNG_AND_B_BURGDORFERI_INF_ENDOTHELIAL_CELL_UP SYSTEMATIC_NAME M6718 COLLECTION C7:IMMUNESIGDB MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/GSE6092_IFNG_VS_IFNG_AND_B_BURGDORFERI_INF_ENDOTHELIAL_CELL_UP NAMESPACE HUMAN_GENE_SYMBOL DESCRIPTION_BRIEF Genes up-regulated in endothelial cells: IFNG [GeneID=3459] versus IFNG [GeneID=3459] and B. burgdoferi. DESCRIPTION_FULL Borrelia burgdorferi, the agent of Lyme disease, promotes pro-inflammatory changes in endothelium that lead to the recruitment of leukocytes. The host immune response to infection results in increased levels of IFN-gamma in the serum and lesions of Lyme disease patients that correlate with greater severity of disease. Therefore, the effect of IFN-gamma on the gene expression profile of primary human endothelial cells exposed to B. burgdorferi was determined. B. burgdorferi and IFN-gamma synergistically augmented the expression of 34 genes, seven of which encode chemokines. Six of these (CCL7, CCL8, CX3CL1, CXCL9, CXCL10, and CXCL11) attract T lymphocytes, and one (CXCL2) is specific for neutrophils. Synergistic production of the attractants for T cells was confirmed at the protein level. IL-1beta, TNF-alpha, and LPS also cooperated with IFN-gamma to induce synergistic production of CXCL10 by endothelium, indicating that IFN-gamma potentiates inflammation in concert with a variety of mediators. An in vitro model of the blood vessel wall revealed that an increased number of human T lymphocytes traversed endothelium exposed to B. burgdorferi and IFN-gamma, as compared to unstimulated endothelial monolayers. In contrast, addition of IFN-gamma diminished the migration of neutrophils across B. burgdorferi-activated endothelium. IFN-gamma thus alters gene expression by endothelium exposed to B. burgdorferi in a manner that promotes recruitment of T cells and suppresses that of neutrophils. This modulation may facilitate the development of chronic inflammatory lesions in Lyme disease. PMID 17202382 GEOID GSE6092 AUTHORS Dame TM,Orenzoff BL,Palmer LE,Furie MB CONTRIBUTOR Jernej Godec CONTRIBUTOR_ORG Dana-Farber Cancer Institute EXACT_SOURCE GSE6092_3469_200_UP FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS 1110032A03Rik,1700025G04Rik,2010002N04Rik,2210404O07Rik,2310022A10Rik,4922501C03Rik,5031439G07Rik,5430435G22Rik,6430548M08Rik,6720401G13Rik,A930001N09Rik,AAAS,ABCG3,ABL1,ACOT2,ACSS1,ADAM22,ADAM8,ADAMTS10,ADI1,AF251705,AI480653,ALDH9A1,ALOX5AP,ANGPTL4,APH1C,APPL2,ARL11,ATP13A2,ATP6V0A1,BBS9,BC031353,BHLHE41,BIN1,BTBD2,C230081A13Rik,CAPRIN2,CBR3,CCDC52,CCPG1,CD180,CD200R1,CD81,CD84,CELF2,CERK,CNR2,CSRNP2,CTDSP2,CUX1,CYB5R1,D6WSU116E,DUSP22,DUSP3,DUSP7,EIF4B,EIF4G3,ENGASE,EXOC6,EZH1,F630110N24Rik,FAM160A2,FAM3C,FAM84B,FBLIM1,FBXO21,FLI1,FMNL1,FNIP2,FRAT1,FRY,GATSL2,GLUL,GNA12,GOT2,GPRC5C,GSN,GTF2I,GYG,HIP1,HIPK1,HP1BP3,HS2ST1,IFT74,IL6ST,IL7R,IRF8,ITFG3,ITGA6,JHDM1D,KANK2,KCNJ10,KHK,KIF13B,KIF21B,KITL,LBH,LFNG,LIMK2,LPAR5,LPXN,LRMP,LTA4H,MAN1C1,MAP3K1,MAVS,MBLAC2,MBP,MCCC2,MCM7,MCOLN1,MFSD11,MIB2,MMP19,MOCOS,MOSPD1,MPEG1,MTVR2,NAGLU,NCOA3,NEK6,NME3,NQO2,NR2C2,PAQR7,PCMTD1,PCYOX1,PDXK,PER3,PHKA2,PINK1,PIP4K2A,PLAG1,PLEKHG3,PLEKHM1,PLEKHM2,PLEKHO1,PLOD1,PLXNB2,PNPO,POLG2,PPARD,PQLC1,PRKACB,PTGS1,PTPN22,PXMP4,RAB3A,RAB3IL1,RAD51L3,RGS10,RGS2,RHOJ,RUNDC2A,S1PR1,SEC22C,SEPX1,SESN1,SGPP1,SKI,SLC16A6,SLC27A1,SLC45A4,SMPD2,SNX24,SNX30,SNX33,SNX8,SOCS6,SORD,STAMBPL1,STRBP,STX3,SYNGR1,TBXAS1,TEC,TIAM1,TLR4,TMC6,TMEM65,TMEM8,TNFAIP8L2,TNRC6B,TPCN1,TPCN2,TPRA1,TRP53INP1,TSC22D3,TSPAN4,TTLL5,TTYH2,VGLL4,WDR45,WWP1,XPR1,ZC4H2,ZDHHC14,ZFP36L2,ZFP608,ZFYVE26 GENE_SYMBOLS ,,,,,,,,,,,AAAS,,ABL1,ACOT2,ACSS1,ADAM22,ADAM8,ADAMTS10,ADI1,CD300C,XXYLT1,ALDH9A1,ALOX5AP,ANGPTL4,,APPL2,ARL11,ATP13A2,ATP6V0A1,BBS9,ATOSA,BHLHE41,BIN1,BTBD2,,CAPRIN2,CBR3,SPICE1,CCPG1,CD180,CD200R1,CD81,CD84,CELF2,CERK,CNR2,CSRNP2,CTDSP2,CUX1,CYB5R1,,DUSP22,DUSP3,DUSP7,EIF4B,EIF4G3,ENGASE,EXOC6,EZH1,,FHIP1B,FAM3C,LRATD2,FBLIM1,FBXO21,FLI1,FMNL1,FNIP2,FRAT1,FRY,CASTOR2,GLUL,GNA12,GOT2,GPRC5C,GSN,GTF2I,GYG1,HIP1,HIPK1,HP1BP3,HS2ST1,IFT74,IL6ST,IL7R,IRF8,FAM234A,ITGA6,KDM7A,KANK2,KCNJ10,KHK,KIF13B,KIF21B,,LBH,LFNG,LIMK2,LPAR5,LPXN,IRAG2,LTA4H,MAN1C1,MAP3K1,MAVS,MBLAC2,MBP,MCCC2,MCM7,MCOLN1,MFSD11,MIB2,MMP19,MOCOS,MOSPD1,MPEG1,,NAGLU,NCOA3,NEK6,NME3,NQO2,NR2C2,PAQR7,PCMTD1,PCYOX1,PDXK,PER3,PHKA2,PINK1,PIP4K2A,PLAG1,PLEKHG3,PLEKHM1,PLEKHM2,PLEKHO1,PLOD1,PLXNB2,PNPO,POLG2,PPARD,SLC66A2,PRKACB,PTGS1,PTPN22,PXMP4,RAB3A,RAB3IL1,RAD51D,RGS10,RGS2,RHOJ,SNX29,S1PR1,SEC22C,MSRB1,SESN1,SGPP1,SKI,SLC16A6,SLC27A1,SLC45A4,SMPD2,SNX24,SNX30,SNX33,SNX8,SOCS6,SORD,STAMBPL1,STRBP,STX3,SYNGR1,TBXAS1,TEC,TIAM1,TLR4,TMC6,TMEM65,PGAP6,TNFAIP8L2,TNRC6B,TPCN1,TPCN2,TPRA1,,TSC22D3,TSPAN4,TTLL5,TTYH2,VGLL4,WDR45,WWP1,XPR1,ZC4H2,ZDHHC14,ZFP36L2,,ZFYVE26 FOUNDER_NAMES