STANDARD_NAME	GUENTHER_GROWTH_SPHERICAL_VS_ADHERENT_UP
SYSTEMATIC_NAME	M7184
COLLECTION	C2:CGP
MSIGDB_URL	https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/GUENTHER_GROWTH_SPHERICAL_VS_ADHERENT_UP
NAMESPACE	AFFY_HG_U133
DESCRIPTION_BRIEF	Genes up-regulated in glioblastoma cell lines displaying spherical growth (cluster-1) compared to those displaying semiadherent or adherent growth phenotype (cluster-2).
DESCRIPTION_FULL	Tumor cells with stem cell-like properties can be cultured from human glioblastomas by using conditions that select for the expansion of neural stem cells. We generated cell lines from glioblastoma specimens with the goal to obtain model systems for glioma stem cell biology. Unsupervised analysis of the expression profiles of nine cell lines established under neural stem cell conditions yielded two distinct clusters. Four cell lines were characterized by the expression of neurodevelopmental genes. They showed a multipotent differentiation profile along neuronal, astroglial and oligodendroglial lineages, grew spherically in vitro, expressed CD133 and formed highly invasive tumors in vivo. The other five cell lines shared expression signatures enriched for extracellular matrix-related genes, had a more restricted differentiation capacity, contained no or fewer CD133+ cells, grew semiadherent or adherent in vitro and displayed reduced tumorigenicity and invasion in vivo. Our findings show that stable, multipotent glioblastoma cell lines with a full stem-like phenotype express neurodevelopmental genes as a distinctive feature, which may offer therapeutic targeting opportunities. The generation of another distinct cluster of cell lines showing similarly homogeneous profiling but restricted stem cell properties suggests that different phenotypes exist, each of which may lead to the typical appearance of glioblastoma.
PMID	18037961
GEOID	GSE8049
AUTHORS	Günther HS,Schmidt NO,Phillips HS,Kemming D,Kharbanda S,Soriano R,Modrusan Z,Meissner H,Westphal M,Lamszus K
CONTRIBUTOR	Jessica Robertson
CONTRIBUTOR_ORG	MSigDB Team
EXACT_SOURCE	Table 2S, 3S: up in cluster-1, down in cluster-2
FILTERED_BY_SIMILARITY	
EXTERNAL_NAMES_FOR_SIMILAR_TERMS	
EXTERNAL_DETAILS_URL	
SOURCE_MEMBERS	200951_s_at,200952_s_at,200953_s_at,202517_at,204029_at,204469_at,204966_at,205344_at,205358_at,206083_at,206330_s_at,206582_s_at,209866_s_at,210015_s_at,213824_at,213825_at,218952_at,219051_x_at,219537_x_at,221261_x_at,223313_s_at,226029_at,226446_at,228170_at,229346_at,236538_at,239230_at
GENE_SYMBOLS	CCND2,CCND2,CCND2,CRMP1,CELSR2,PTPRZ1,ADGRB2,CSPG5,GRIA2,ADGRB1,SHC3,ADGRG1,ADGRL3,MAP2,OLIG2,OLIG2,PCSK1N,METRN,DLL3,MAGED4B,MAGED4B,VANGL2,HES6,OLIG1,NES,GRIA2,HES5
FOUNDER_NAMES