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<MSIGDB BUILD_DATE="Jan 29, 2026" VERSION="2026.1.Hs" NAME="export">
    <GENESET STANDARD_NAME="HARALAMBIEVA_PBMC_DRYVAX_AGE_18_40YO_STIMULATED_VS_UNSTIMULATED_9_TO_34MO_UP" SYSTEMATIC_NAME="M41189" HISTORICAL_NAMES="" PMID="22949304" AUTHORS="Haralambieva IH,Oberg AL,Dhiman N,Ovsyannikova IG,Kennedy RB,Grill DE,Jacobson RM,Poland GA" GEOID="" EXACT_SOURCE="Table 2" GENESET_LISTING_URL="" EXTERNAL_DETAILS_URL="https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3475634/table/JIS546TB2/" CHIP="HUMAN_GENE_SYMBOL" CONTRIBUTOR="HIPC SIGNATURES" CONTRIBUTOR_ORG="NIAID/HIPC SIGNATURES" DESCRIPTION_BRIEF="Genes up-regulated in peripheral blood mononuclear cell stimulated vs unstimulated in adults (18-40) after exposure to Dryvax , time point 9 to 34M. Comment: Original exposure within previous 4 years" DESCRIPTION_FULL="BACKGROUND: The mechanisms underlying smallpox vaccine-induced variations in immune responses are not well understood, but are of considerable interest to a deeper understanding of poxvirus immunity and correlates of protection. METHODS: We assessed transcriptional messenger RNA expression changes in 197 recipients of primary smallpox vaccination representing the extremes of humoral and cellular immune responses. RESULTS: The 20 most significant differentially expressed genes include a tumor necrosis factor-receptor superfamily member, an interferon (IFN) gene, a chemokine gene, zinc finger protein genes, nuclear factors, and histones (P &lt;= 1.06E(-20), q &lt;= 2.64E(-17)). A pathway analysis identified 4 enriched pathways with cytokine production by the T-helper 17 subset of CD4+ T cells being the most significant pathway (P = 3.42E(-05)). Two pathways (antiviral actions of IFNs, P = 8.95E(-05); and IFN-alpha/beta signaling pathway, P = 2.92E(-04)), integral to innate immunity, were enriched when comparing high with low antibody responders (false discovery rate, &lt; 0.05). Genes related to immune function and transcription (TLR8, P =.0002; DAPP1, P =.0003; LAMP3, P = 9.96E(-05); NR4A2, P &lt;= .0002; EGR3, P = 4.52E(-05)), and other genes with a possible impact on immunity (LNPEP, P = 3.72E(-05); CAPRIN1, P =.0001; XRN1, P =.0001), were found to be expressed differentially in high versus low antibody responders. CONCLUSION: We identified novel and known immunity-related genes and pathways that may account for differences in immune response to smallpox vaccination." TAGS="" MEMBERS="CCAR2,CD160,FAM71B,H4C5,HERC5,IFNA1,IKZF1,IKZF2,NEXN,NFE2L3,NR4A2,POLR2C,RSRC1,SOBP,TNFRSF10D,TNK2,XCL1" MEMBERS_SYMBOLIZED="CCAR2,CD160,GARIN3,H4C5,HERC5,IFNA1,IKZF1,IKZF2,NEXN,NFE2L3,NR4A2,POLR2C,RSRC1,SOBP,TNFRSF10D,TNK2,XCL1" MEMBERS_EZID="10188,10320,11126,153745,22807,3439,4929,51191,51319,5432,55084,57805,6375,8367,8793,91624,9603" MEMBERS_MAPPING="CCAR2,CCAR2,57805|CD160,CD160,11126|FAM71B,GARIN3,153745|H4C5,H4C5,8367|HERC5,HERC5,51191|IFNA1,IFNA1,3439|IKZF1,IKZF1,10320|IKZF2,IKZF2,22807|NEXN,NEXN,91624|NFE2L3,NFE2L3,9603|NR4A2,NR4A2,4929|POLR2C,POLR2C,5432|RSRC1,RSRC1,51319|SOBP,SOBP,55084|TNFRSF10D,TNFRSF10D,8793|TNK2,TNK2,10188|XCL1,XCL1,6375" FILTERED_BY_SIMILARITY="" FOUNDER_NAMES="" REFINEMENT_DATASETS="" VALIDATION_DATASETS="" CATEGORY_CODE="C7" ORGANISM="Homo sapiens" SUB_CATEGORY_CODE="VAX"/>
</MSIGDB>
