STANDARD_NAME HARALAMBIEVA_PBMC_FLUARIX_AGE_50_74YO_CORR_WITH_28D_MEM_B_CELL_RESPONSE_AT_28DY_LEUK_MIGR_MAPK_ACT_CYTOK_SIG_DIAB_OF_THE_YNG_POSITIVE SYSTEMATIC_NAME M41177 COLLECTION C7:VAX MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/HARALAMBIEVA_PBMC_FLUARIX_AGE_50_74YO_CORR_WITH_28D_MEM_B_CELL_RESPONSE_AT_28DY_LEUK_MIGR_MAPK_ACT_CYTOK_SIG_DIAB_OF_THE_YNG_POSITIVE NAMESPACE HUMAN_GENE_SYMBOL DESCRIPTION_BRIEF Genes positively correlated with memory B cell response at 28d in peripheral blood mononuclear cell in seniors (50-74) after exposure to Fluarix , time point 28D. Comment: selected pathways: leukocyte migration, MAP kinase activity, cytokine signaling, diabetes of the young DESCRIPTION_FULL BACKGROUND: Studies suggest that the recall-based humoral immune responses to influenza A/H1N1 originates from activated memory B cells. The aim of this study was to identify baseline, early and late blood transcriptional signatures (in peripheral blood mononuclear cells/PBMCs) associated with memory B cell response following influenza vaccination. METHODS: We used pre- and post-vaccination mRNA-Seq transcriptional profiling on samples from 159 subjects (50-74years old) following receipt of seasonal trivalent influenza vaccine containing the A/California/7/2009/H1N1-like virus, and penalized regression modeling to identify associations with influenza A/H1N1-specific memory B cell ELISPOT response after vaccination. RESULTS: Genesets and genes (p-value range 7.92E(-08) to 0.00018, q-value range 0.00019-0.039) demonstrating significant associations (of gene expression levels) with memory B cell response suggest the importance of metabolic (cholesterol and lipid metabolism-related), cell migration/adhesion, MAP kinase, NF-kB cell signaling (chemokine/cytokine signaling) and transcriptional regulation gene signatures in the development of memory B cell response after influenza vaccination. CONCLUSION: Through an unbiased transcriptome-wide profiling approach, our study identified signatures of memory B cell response following influenza vaccination, highlighting the underappreciated role of metabolic changes (among the other immune function-related events) in the regulation of influenza vaccine-induced immune memory. PMID 27317456 GEOID AUTHORS Haralambieva IH,Ovsyannikova IG,Kennedy RB,Zimmermann MT,Grill DE,Oberg AL,Poland GA CONTRIBUTOR HIPC SIGNATURES CONTRIBUTOR_ORG NIAID/HIPC SIGNATURES EXACT_SOURCE Fig 2C-F; Table 2 FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5520794/table/T2/ SOURCE_MEMBERS BHLHA15,CD34,DOCK2,EREG,HES1,IL10,IL8,MAPK12,MAPK14,MAPK7,PF4,SFTPD,SOCS5,TGFB2 GENE_SYMBOLS BHLHA15,CD34,DOCK2,EREG,HES1,IL10,CXCL8,MAPK12,MAPK14,MAPK7,PF4,SFTPD,SOCS5,TGFB2 FOUNDER_NAMES