STANDARD_NAME HARALAMBIEVA_PBMC_M_M_R_II_AGE_13_16YO_STIMULATED_VS_UNSTIMULATED_3_TO_7YR_POST_TOP_DEG_UP SYSTEMATIC_NAME M41076 COLLECTION C7:VAX MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/HARALAMBIEVA_PBMC_M_M_R_II_AGE_13_16YO_STIMULATED_VS_UNSTIMULATED_3_TO_7YR_POST_TOP_DEG_UP NAMESPACE HUMAN_GENE_SYMBOL DESCRIPTION_BRIEF Genes up-regulated in peripheral blood mononuclear cell stimulated vs unstimulated in children (13-16) after exposure to M-M-R II , time point 3 to 7Y. Comment: top differentially expressed genes, full set of 1080 avail in Suppl Materials DESCRIPTION_FULL Immune responses to current rubella vaccines demonstrate significant inter-individual variability. We performed mRNA-Seq profiling on PBMCs from high and low antibody responders to rubella vaccination to delineate transcriptional differences upon viral stimulation. Generalized linear models were used to assess the per gene fold change (FC) for stimulated versus unstimulated samples or the interaction between outcome and stimulation. Model results were evaluated by both FC and p-value. Pathway analysis and self-contained gene set tests were performed for assessment of gene group effects. Of 17,566 detected genes, we identified 1,080 highly significant differentially expressed genes upon viral stimulation (p < 1.00E(-15), FDR < 1.00E(-14)), including various immune function and inflammation-related genes, genes involved in cell signaling, cell regulation and transcription, and genes with unknown function. Analysis by immune outcome and stimulation status identified 27 genes (p <= 0.0006 and FDR <= 0.30) that responded differently to viral stimulation in high vs. low antibody responders, including major histocompatibility complex (MHC) class I genes (HLA-A, HLA-B and B2M with p = 0.0001, p = 0.0005 and p = 0.0002, respectively), and two genes related to innate immunity and inflammation (EMR3 and MEFV with p = 1.46E(-08) and p = 0.0004, respectively). Pathway and gene set analysis also revealed transcriptional differences in antigen presentation and innate/inflammatory gene sets and pathways between high and low responders. Using mRNA-Seq genome-wide transcriptional profiling, we identified antigen presentation and innate/inflammatory genes that may assist in explaining rubella vaccine-induced immune response variations. Such information may provide new scientific insights into vaccine-induced immunity useful in rational vaccine development and immune response monitoring. PMID 23658707 GEOID AUTHORS Haralambieva IH,Oberg AL,Ovsyannikova IG,Kennedy RB,Grill DE,Middha S,Bot BM,Wang VW,Smith DI,Jacobson RM,Poland GA CONTRIBUTOR HIPC SIGNATURES CONTRIBUTOR_ORG NIAID/HIPC SIGNATURES EXACT_SOURCE Table 2 FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3641062/table/pone-0062149-t002/ SOURCE_MEMBERS AQP9,ARHGEF28,ARRDC4,B3GNT5,CCL18,CCL23,CLEC1A,CXCL6,DLC1,ENPP2,FAM135B,FAM20A,FFAR4,FGD5,FN1,GNG12,IL1B,LYVE1,MLXIPL,NRP1,OLR1,PCOLCE2,PDPN,PPIC,PPP1R14C,PROCR,S1PR3,SERPINA1,SERPINE1,SGMS2,SHROOM4,SIGLEC11,SLC39A8,SPINT1,SPRED1,TIMP4,TMEM52B,TMTC1,TNFAIP8L3,TNFRSF12A,TREM1,VSIG4 GENE_SYMBOLS AQP9,ARHGEF28,ARRDC4,B3GNT5,CCL18,CCL23,CLEC1A,CXCL6,DLC1,ENPP2,FAM135B,FAM20A,FFAR4,FGD5,FN1,GNG12,IL1B,LYVE1,MLXIPL,NRP1,OLR1,PCOLCE2,PDPN,PPIC,PPP1R14C,PROCR,S1PR3,SERPINA1,SERPINE1,SGMS2,SHROOM4,SIGLEC11,SLC39A8,SPINT1,SPRED1,TIMP4,TMEM52B,TMTC1,TNFAIP8L3,TNFRSF12A,TREM1,VSIG4 FOUNDER_NAMES