STANDARD_NAME HOEK_MYELOID_DENDRITIC_CELL_2011_2012_TIV_ADULT_7DY_UP SYSTEMATIC_NAME M41009 COLLECTION C7:VAX MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/HOEK_MYELOID_DENDRITIC_CELL_2011_2012_TIV_ADULT_7DY_UP NAMESPACE HUMAN_GENE_SYMBOL DESCRIPTION_BRIEF Genes up-regulated in myeloid dendritic cell 7d vs 0d in adults after exposure to 2011-2012 trivalent inactivated vaccine (A/California/7/09 (H1N1), A/Perth /16/2009 (H3N2), B/Brisbane/60/2008) , time point 7D. Comment: Up-regulated DE RNA transcripts (up >= 1.5x) shared between both TIV-vaccinated donors DESCRIPTION_FULL Systems biology is an approach to comprehensively study complex interactions within a biological system. Most published systems vaccinology studies have utilized whole blood or peripheral blood mononuclear cells (PBMC) to monitor the immune response after vaccination. Because human blood is comprised of multiple hematopoietic cell types, the potential for masking responses of under-represented cell populations is increased when analyzing whole blood or PBMC. To investigate the contribution of individual cell types to the immune response after vaccination, we established a rapid and efficient method to purify human T and B cells, natural killer (NK) cells, myeloid dendritic cells (mDC), monocytes, and neutrophils from fresh venous blood. Purified cells were fractionated and processed in a single day. RNA-Seq and quantitative shotgun proteomics were performed to determine expression profiles for each cell type prior to and after inactivated seasonal influenza vaccination. Our results show that transcriptomic and proteomic profiles generated from purified immune cells differ significantly from PBMC. Differential expression analysis for each immune cell type also shows unique transcriptomic and proteomic expression profiles as well as changing biological networks at early time points after vaccination. This cell type-specific information provides a more comprehensive approach to monitor vaccine responses. PMID 25706537 GEOID AUTHORS Hoek KL,Samir P,Howard LM,Niu X,Prasad N,Galassie A,Liu Q,Allos TM,Floyd KA,Guo Y,Shyr Y,Levy SE,Joyce S,Edwards KM,Link AJ CONTRIBUTOR HIPC SIGNATURES CONTRIBUTOR_ORG NIAID/HIPC SIGNATURES EXACT_SOURCE S6 Dataset FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4338067/bin/pone.0118528.s006.xlsx SOURCE_MEMBERS ADAMTS10,ADORA1,B3GNT7,BOLA2B,BTLA,CARD11,CCDC65,CCR5AS,CD2,CLEC9A,DPP4,GPX7,IDO1,KIT,KLRB1,LPL,MACORIS,MATK,MSLN,PRKCH,PTX3,S100A12,SEPTIN1,SEPTIN3,SIGLEC6,TLE1,TRBV7-3 GENE_SYMBOLS ADAMTS10,ADORA1,B3GNT7,BOLA2B,BTLA,CARD11,CCDC65,CCR5AS,CD2,CLEC9A,DPP4,GPX7,IDO1,KIT,KLRB1,LPL,MACORIS,MATK,MSLN,PRKCH,PTX3,S100A12,SEPTIN1,SEPTIN3,SIGLEC6,TLE1,TRBV7-3 FOUNDER_NAMES