STANDARD_NAME	HWANG_PROSTATE_CANCER_MARKERS
SYSTEMATIC_NAME	M6195
COLLECTION	C2:CGP
MSIGDB_URL	https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/HWANG_PROSTATE_CANCER_MARKERS
NAMESPACE	Human_NCBI_Gene_ID
DESCRIPTION_BRIEF	Proteins implicated in prostate carcinogenesis.
DESCRIPTION_FULL	Successful treatment of multiple cancer types requires early detection and identification of reliable biomarkers present in specific cancer tissues. To test the feasibility of identifying proteins from archival cancer tissues, we have developed a methodology, termed direct tissue proteomics (DTP), which can be used to identify proteins directly from formalin-fixed paraffin-embedded prostate cancer tissue samples. Using minute prostate biopsy sections, we demonstrate the identification of 428 prostate-expressed proteins using the shotgun method. Because the DTP method is not quantitative, we employed the absolute quantification method and demonstrate picogram level quantification of prostate-specific antigen. In depth bioinformatics analysis of these expressed proteins affords the categorization of metabolic pathways that may be important for distinct stages of prostate carcinogenesis. Furthermore, we validate Wnt-3 as an upregulated protein in cancerous prostate cells by immunohistochemistry. We propose that this general strategy provides a roadmap for successful identification of critical molecular targets of multiple cancer types.
PMID	16799640
GEOID	
AUTHORS	Hwang SI,Thumar J,Lundgren DH,Rezaul K,Mayya V,Wu L,Eng J,Wright ME,Han DK
CONTRIBUTOR	Arthur Liberzon
CONTRIBUTOR_ORG	MSigDB Team
EXACT_SOURCE	Table 3S
FILTERED_BY_SIMILARITY	
EXTERNAL_NAMES_FOR_SIMILAR_TERMS	
EXTERNAL_DETAILS_URL	
SOURCE_MEMBERS	10397,10516,10551,11315,1191,1499,2289,2316,3310,3611,3837,3956,3958,4282,4582,5230,5245,5292,55522,563,5728,5901,7295,7430,7473,811,85004,89780,8991
GENE_SYMBOLS	NDRG1,FBLN5,AGR2,PARK7,CLU,CTNNB1,FKBP5,FLNA,HSPA6,ILK,KPNB1,LGALS1,LGALS3,MIF,MUC1,PGK1,PHB1,PIM1,ILK,AZGP1,PTEN,RAN,TXN,EZR,WNT3,CALR,RERG,WNT3A,SELENBP1
FOUNDER_NAMES