STANDARD_NAME	JAEGER_METASTASIS_UP
SYSTEMATIC_NAME	M5740
COLLECTION	C2:CGP
MSIGDB_URL	https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/JAEGER_METASTASIS_UP
NAMESPACE	AFFY_HG_U133
DESCRIPTION_BRIEF	Genes up-regulated in metastases from malignant melanoma compared to the primary tumors.
DESCRIPTION_FULL	PURPOSE: To better understand the molecular mechanisms of malignant melanoma progression and metastasis, gene expression profiling was done of primary melanomas and melanoma metastases. EXPERIMENTAL DESIGN: Tumor cell-specific gene expression in 19 primary melanomas and 22 melanoma metastases was analyzed using oligonucleotide microarrays after laser-capture microdissection of melanoma cells. Statistical analysis was done by random permutation analysis and support vector machines. Microarray data were further validated by immunohistochemistry and immunoblotting. RESULTS: Overall, 308 genes were identified that showed significant differential expression between primary melanomas and melanoma metastases (false discovery rate<or=0.05). Significantly overrepresented gene ontology categories in the list of 308 genes were cell cycle regulation, mitosis, cell communication, and cell adhesion. Overall, 47 genes showed up-regulation in metastases. These included Cdc6, Cdk1, septin 6, mitosin, kinesin family member 2C, osteopontin, and fibronectin. Down-regulated genes included E-cadherin, fibroblast growth factor binding protein, and desmocollin 1 and desmocollin 3, stratifin/14-3-3sigma, and the chemokine CCL27. Using support vector machine analysis of gene expression data, a performance of >85% correct classifications for primary melanomas and metastases was reached. Further analysis showed that subtypes of primary melanomas displayed characteristic gene expression patterns, as do thin tumors (<or=1.0 mm Breslow thickness) compared with intermediate and thick tumors (>2.0 mm Breslow thickness). CONCLUSIONS: Taken together, this large-scale gene expression study of malignant melanoma identified molecular signatures related to metastasis, melanoma subtypes, and tumor thickness. These findings not only provide deeper insights into the pathogenesis of melanoma progression but may also guide future research on innovative treatments.
PMID	17289871
GEOID	
AUTHORS	Jaeger J,Koczan D,Thiesen HJ,Ibrahim SM,Gross G,Spang R,Kunz M
CONTRIBUTOR	Leona Saunders
CONTRIBUTOR_ORG	MSigDB Team
EXACT_SOURCE	Table 2S
FILTERED_BY_SIMILARITY	
EXTERNAL_NAMES_FOR_SIMILAR_TERMS	
EXTERNAL_DETAILS_URL	
SOURCE_MEMBERS	201261_x_at,201387_s_at,201521_s_at,201663_s_at,201664_at,202487_s_at,202508_s_at,202609_at,202620_s_at,202783_at,202784_s_at,202856_s_at,202952_s_at,202983_at,203060_s_at,203434_s_at,203435_s_at,203510_at,203968_s_at,204030_s_at,204170_s_at,204597_x_at,204776_at,205066_s_at,206028_s_at,207828_s_at,208079_s_at,208308_s_at,209094_at,209374_s_at,209408_at,209773_s_at,209875_s_at,209942_x_at,210467_x_at,210495_x_at,211719_x_at,211767_at,212350_at,212353_at,212354_at,212414_s_at,212464_s_at,212843_at,213790_at,213905_x_at,214023_x_at,214511_x_at,214603_at,214612_x_at,216442_x_at,218308_at,218375_at,218585_s_at,218662_s_at,218663_at,220445_s_at
GENE_SYMBOLS	BGN,UCHL1,NCBP2,SMC4,SMC4,H2AZ2,SNAP25,EPS8,PLOD2,NNT,NNT,SLC16A3,ADAM12,HLTF,PAPSS2,MME,MME,MET,CDC6,SCHIP1,CKS2,STC1,THBS4,ENPP1,MERTK,CENPF,AURKA,GPI,DDAH1,IGHM,KIF2C,RRM2,SPP1,MAGEA3,MAGEA12,FN1,FN1,GINS4,TBC1D1,SULF1,SULF1,SEPTIN6,FN1,NCAM1,ADAM12,BGN,TUBB2B,FCGR1A,MAGEA2,MAGEA3,FN1,TACC3,NUDT9,DTL,NCAPG,NCAPG,CSAG3
FOUNDER_NAMES