STANDARD_NAME KALMA_E2F1_TARGETS SYSTEMATIC_NAME M10142 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/KALMA_E2F1_TARGETS NAMESPACE HUMAN_GENE_SYMBOL DESCRIPTION_BRIEF DNA replication genes up-regulated in a Rat-1a cell line (fibroblast) by expression of E2F1 [GeneID=1869]. DESCRIPTION_FULL The transcription factor E2F-1 plays a pivotal role in the regulation of G1/S transition in higher eukaryotes cell cycle. We used a cell line containing an inducible E2F-1 and oligonucleotide microarray analysis to identify novel E2F target genes. We show that E2F-1 up-regulates the expression of a number of genes coding for components of the DNA replication machinery. Among them is the gene coding for the 32 Kd subunit of replication protein A (RPA2). Replication protein A is the most abundant single strand DNA binding complex and it is essential for DNA replication. We demonstrate that RPA2 is a novel E2F target gene whose expression can be directly regulated by E2F-1 via E2F binding sites in its promoter. In addition, expression of Topoisomerase IIalpha and subunit IV of DNA polymerase alpha is also up-regulated upon E2F-1 induction. Taken together, these results provide novel links between components of the DNA replication machinery and the cell growth regulatory pathway involving the Rb tumor suppressor and E2F. PMID 11313881 GEOID AUTHORS Kalma Y,Marash L,Lamed Y,Ginsberg D CONTRIBUTOR John Newman CONTRIBUTOR_ORG University of Washington EXACT_SOURCE Table 1: full names changed to symbols FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS CCNC,FEN1,MCM2,MCM6,PCNA,POLA2,POLD1,RFC4,RPA2,TOP2A,TYMS GENE_SYMBOLS CCNC,FEN1,MCM2,MCM6,PCNA,POLA2,POLD1,RFC4,RPA2,TOP2A,TYMS FOUNDER_NAMES