STANDARD_NAME KANG_DOXORUBICIN_RESISTANCE_UP SYSTEMATIC_NAME M1037 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/KANG_DOXORUBICIN_RESISTANCE_UP NAMESPACE AFFY_HG_U133 DESCRIPTION_BRIEF Genes up-regulated in gastric cancer cell lines: doxorubicin [PubChem=31703] resistant vs sensitive. DESCRIPTION_FULL PURPOSE: A major obstacle in chemotherapy is treatment failure due to anticancer drug resistance. The emergence of acquired resistance results from host factors and genetic or epigenetic changes in the cancer cells. The purpose of this study was to identify differentially expressed genes associated with acquisition of resistance in human gastric cancer cells. EXPERIMENTAL DESIGN: We performed global gene expression analysis in the acquired drug-resistant gastric cancer cell lines to the commonly used drugs 5-fluorouracil, doxorubicin, and cisplatin using Affymetrix HG-U133A microarray. The gene expression patterns of 10 chemoresistant gastric cancer cell lines were compared with those of four parent cell lines using fold-change and Wilcoxon's test for data analysis. RESULTS: We identified over 250 genes differentially expressed in 5-fluorouracil-, cisplatin-, or doxorubicin-resistant gastric cancer cell lines. Our expression analysis also identified eight multidrug resistance candidate genes that were associated with resistance to two or more of the tested chemotherapeutic agents. Among these, midkine (MDK), a heparin-binding growth factor, was overexpressed in all drug-resistant cell lines, strongly suggesting that MDK might contribute to multidrug resistance in gastric cancer cells. CONCLUSIONS: Our investigation provides comprehensive gene information associated with acquired resistance to anticancer drugs in gastric cancer cells and a basis for additional functional studies. PMID 14734480 GEOID AUTHORS Kang HC,Kim IJ,Park JH,Shin Y,Ku JL,Jung MS,Yoo BC,Kim HK,Park JG CONTRIBUTOR John Newman CONTRIBUTOR_ORG University of Washington EXACT_SOURCE Table 3, 3A FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS 201292_at,201467_s_at,201477_s_at,202095_s_at,202240_at,202338_at,202503_s_at,202580_x_at,202589_at,202768_at,202805_s_at,202870_s_at,203214_x_at,203270_at,203276_at,203362_s_at,203755_at,203967_at,204023_at,204026_s_at,204146_at,204441_s_at,204538_x_at,204886_at,204962_s_at,205345_at,206102_at,207165_at,208079_s_at,208808_s_at,209053_s_at,209642_at,209714_s_at,209773_s_at,210052_s_at,212021_s_at,212621_at,213007_at,213599_at,218009_s_at,218039_at,218115_at,218355_at,218663_at,218726_at,218741_at,218782_s_at,219000_s_at,219148_at,219510_at,219588_s_at,219918_s_at,221520_s_at,222037_at GENE_SYMBOLS TOP2A,NQO1,RRM1,BIRC5,PLK1,TK1,PCLAF,FOXM1,TYMS,FOSB,ABCC1,CDC20,CDK1,DTYMK,LMNB1,MAD2L1,BUB1B,CDC6,RFC4,ZWINT,RAD51AP1,POLA2,NPIPA1,PLK4,CENPA,BARD1,GINS1,HMMR,AURKA,HMGB2,NSD2,BUB1,CDKN3,RRM2,TPX2,MKI67,NEMP1,FANCI,OIP5,PRC1,NUSAP1,ASF1B,KIF4A,NCAPG,HJURP,CENPM,ATAD2,DSCC1,PBK,POLQ,NCAPG2,ASPM,CDCA8,MCM4 FOUNDER_NAMES