STANDARD_NAME	KIM_WT1_TARGETS_8HR_DN
SYSTEMATIC_NAME	M9948
COLLECTION	C2:CGP
MSIGDB_URL	https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/KIM_WT1_TARGETS_8HR_DN
NAMESPACE	AFFY_HG_U133
DESCRIPTION_BRIEF	Genes down-regulated in UB27 cells (osteosarcoma) at 8 hr after inducing the expression of a mutant form of WT1 [GeneID=7490].
DESCRIPTION_FULL	The Wilms' tumor suppressor gene (WT1) encodes a zinc finger transcription factor that is vital during development of several organs including metanephric kidneys. Despite the critical regulatory role of WT1, the pathways and mechanisms by which WT1 orchestrates development remain elusive. To identify WT1 target genes, we performed a genome-wide expression profiling analysis in cells expressing inducible WT1. We identified a number of direct WT1 target genes, including the epidermal growth factor (EGF)-family ligands epiregulin and HB-EGF, the chemokine CX3CL1, and the transcription factors SLUG and JUNB. The target genes were validated using quantitative reverse transcriptase-polymerase chain reaction, small interfering RNA knockdowns, chromatin immunoprecipitation, and luciferase reporter analyses. Immunohistochemistry of fetal kidneys confirmed that a number of the WT1 target genes had overlapping expression patterns with the highly restricted spatiotemporal expression of WT1. Finally, using an in vitro embryonic kidney culture assay, we found that the addition of recombinant epiregulin, amphiregulin, CX3CL1, and interleukin-11 significantly enhanced ureteric bud branching morphogenesis. Our genome-wide screen implicates WT1 in the transcriptional regulation of the EGF-family of growth factors as well as the CX3CL1 chemokine during nephrogenesis.
PMID	17430890
GEOID	GSE5117
AUTHORS	Kim HS,Kim MS,Hancock AL,Harper JC,Park JY,Poy G,Perantoni AO,Cam M,Malik K,Lee SB
CONTRIBUTOR	Arthur Liberzon
CONTRIBUTOR_ORG	MSigDB Team
EXACT_SOURCE	Table 1S
FILTERED_BY_SIMILARITY	
EXTERNAL_NAMES_FOR_SIMILAR_TERMS	
EXTERNAL_DETAILS_URL	
SOURCE_MEMBERS	1861_at,201043_s_at,201679_at,201939_at,202237_at,202238_s_at,202298_at,202323_s_at,202326_at,202524_s_at,202527_s_at,202655_at,202693_s_at,202736_s_at,202976_s_at,203080_s_at,203108_at,203112_s_at,203123_s_at,203489_at,203606_at,203637_s_at,203643_at,203789_s_at,203820_s_at,203860_at,204298_s_at,204948_s_at,205048_s_at,205195_at,205401_at,205807_s_at,205822_s_at,205994_at,206163_at,206237_s_at,206239_s_at,206290_s_at,206295_at,206343_s_at,206442_at,206493_at,206560_s_at,206787_at,207250_at,207262_at,207416_s_at,207612_at,207734_at,207828_s_at,207857_at,207980_s_at,207993_s_at,208045_at,208473_s_at,208650_s_at,208765_s_at,208937_s_at,209059_s_at,209101_at,209135_at,209202_s_at,209328_x_at,209464_at,209595_at,209754_s_at,209836_x_at,209908_s_at,209909_s_at,209964_s_at,210197_at,210220_at,210288_at,210410_s_at,210500_at,210650_s_at,210663_s_at,210667_s_at,210694_s_at,210717_at,210792_x_at,211366_x_at,211421_s_at,211513_s_at,211798_x_at,212977_at,213048_s_at,213104_at,213284_at,213506_at,214006_s_at,214032_at,214282_at,214371_at,214498_at,214657_s_at,214770_at,215154_at,215199_at,215210_s_at,215252_at,215280_s_at,215377_at,215560_x_at,215697_at,215720_s_at,215778_x_at,215943_at,216194_s_at,216202_s_at,216541_x_at,216666_at,216669_at,216884_at,217278_x_at,217293_at,217325_at,217552_x_at,217618_x_at,217747_s_at,217836_s_at,218194_at,218279_s_at,218490_s_at,218700_s_at,219014_at,219179_at,219522_at,219534_x_at,219653_at,219786_at,219927_at,220222_at,220376_at,220407_s_at,220576_at,220768_s_at,220979_s_at,221452_s_at,221477_s_at,221591_s_at,221646_s_at,221989_at,222035_s_at,222108_at,222131_x_at,40148_at,54037_at,AFFX-HUMRGE/M10098_3_at,AFFX-HUMRGE/M10098_5_at,AFFX-M27830_5_at,AFFX-r2-Hs18SrRNA-3_s_at,AFFX-r2-Hs28SrRNA-3_at,AFFX-r2-Hs28SrRNA-5_at,AFFX-r2-Hs28SrRNA-M_at
GENE_SYMBOLS	BAD,ANP32A,SRRT,PLK2,NNMT,NNMT,NDUFA1,ACBD3,EHMT2,SPOCK2,SMAD4,MANF,STK17A,LSM4,RHOBTB3,BAZ2B,GPRC5A,NELFA,SLC11A2,SIVA1,NDUFS6,MID1,ERF,SEMA3C,IGF2BP3,PCCA,LOX,FST,PSPH,AP1S1,AGPS,TUFT1,HMGCS1,ELK4,MAB21L1,NRG1,SPINK1,RGS7,IL18,NRG1,SEMG1,ITGA2B,MIA,BRDT,SIX6,APOF,NFATC3,WNT8B,LAX1,CENPF,LILRA2,CITED2,CHP1,,GP2,CD24P2,HNRNPR,ID1,EDF1,CCN2,ASPH,EXTL3,HIGD2A,AURKB,GTF2F2,TMPO,BOLA2,TGFB2,TGFB2,ATXN7,ITPK1,FZD2,KLRG1,MSH5,,PCLO,KYNU,GATD3,ZNF514,,SIVA1,CASP1,RET,OGFR,,ACKR3,,TSR3,,F2RL1,GGCX,ZAP70,,TSSK2,ASIP,NEAT1,MSR1,ULK2,CALD1,DLST,,PPFIA3,,RGS17,,NFYA,,,TBCB,SPTLC2,,MGAM2,,,,,KRT3,CR1,HUS1,RPS9,YY1AP1,REXO2,,ZNF302,RAB29,PLAC8,DACT1,FJX1,CDKN1C,LSM14B,TESMIN,FCF1,RBM12B-AS1,LRRC19,TGFB2,PGAP1,CSNK1G3,ST6GALNAC5,TMEM14B,SOD2,PIMREG,ZDHHC11,RPL10,PAPOLA,AMIGO2,RHOT2,APBB2,HPS4,,,,,,,
FOUNDER_NAMES