STANDARD_NAME KOHOUTEK_CCNT1_TARGETS SYSTEMATIC_NAME M2401 COLLECTION C2:CGP MSIGDB_URL https://www.gsea-msigdb.org/gsea/msigdb/human/geneset/KOHOUTEK_CCNT1_TARGETS NAMESPACE MOUSE_GENE_SYMBOL DESCRIPTION_BRIEF Genes down-regulated in E14 ES (embryonic stem) cells upon knockdown of CYCT1 [GeneID=904] by RNAi. DESCRIPTION_FULL The positive transcription elongation factor b (P-TEFb) is essential for the elongation of transcription and cotranscriptional processing by RNA polymerase II. In mammals, it contains predominantly the C-type cyclin cyclin T1 (CycT1) or CycT2 and cyclin-dependent kinase 9 (Cdk9). To determine if these cyclins have redundant functions or affect distinct sets of genes, we genetically inactivated the CycT2 gene (Ccnt2) using the beta-galactosidase-neomycin gene (beta-geo) gene trap technology in the mouse. Visualizing beta-galactosidase during mouse embryogenesis revealed that CycT2 is expressed abundantly during embryogenesis and throughout the organism in the adult. This finding was reflected in the expression of CycT2 in all adult tissues and organs. However, despite numerous matings of heterozygous mice, we observed no CycT2(-/-) embryos, pups, or adult mice. This early lethality could have resulted from decreased expression of critical genes, which were revealed by short interfering RNAs against CycT2 in embryonic stem cells. Thus, CycT1 and CycT2 are not redundant, and these different P-TEFb complexes regulate subsets of distinct genes that are important for embryonic development. PMID 19364821 GEOID AUTHORS Kohoutek J,Li Q,Blazek D,Luo Z,Jiang H,Peterlin BM CONTRIBUTOR Arthur Liberzon CONTRIBUTOR_ORG MSigDB Team EXACT_SOURCE Table 1S: CycT1 siRNA FILTERED_BY_SIMILARITY EXTERNAL_NAMES_FOR_SIMILAR_TERMS EXTERNAL_DETAILS_URL SOURCE_MEMBERS 1300014I06Rik,4930424G05Rik,4933402E13Rik,6030495B01Rik,A930034L06Rik,AA467197,Abcb1b,Abhd14b,Acadvl,Ahnak2,Anxa5,Aqp3,Arl4c,Bbs2,Bbs7,Bmp1,C230093N12Rik,Capns1,Ccnt1,Cd68,Chrnb1,Cldn4,Cln3,Cltb,Col7a1,Cryab,Cxcl16,D330001F17Rik,Dner,EG638695,Enpp5,Fhl2,Galm,Gpx2,Hsd3b7,Htra1,Itgb4,Krt14,Krt18,LOC100046120,LOC381140,LOC381844,LOC386021,Mvp,Mylpf,Pgc,Pros1,Pter,S100a1,S100a13,Sfn,Slc39a4,Sqstm1,Stmn3,Tacstd2,Tbx3,Tex19,Tinagl,Wdr45 GENE_SYMBOLS PXDC1,PATL2,MAGEC2,,SNHG11,C15orf48,ABCB1,ABHD14B,ACADVL,AHNAK2,ANXA5,AQP3,ARL4C,BBS2,BBS7,BMP1,EEIG1,CAPNS1,CCNT1,CD68,CHRNB1,CLDN4,CLN3,CLTB,COL7A1,CRYAB,CXCL16,MROH1,DNER,,ENPP5,FHL2,GALM,GPX2,HSD3B7,HTRA1,ITGB4,KRT14,KRT18,,,,,MVP,MYL11,PGC,PROS1,PTER,S100A1,S100A13,SFN,SLC39A4,SQSTM1,STMN3,TACSTD2,TBX3,TEX19,,WDR45 FOUNDER_NAMES